LOCUS       sequence-308157-        9629 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive lentiviral expression of human CTSB..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9629)
  AUTHORS   Chavez Lab
  TITLE     Coronavirus host receptors and proteases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLEX307-CTSB-G418
FEATURES             Location/Qualifiers
     source          1..9629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2345..2369)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2377..2418
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2469..3057
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2522..2542)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2940..2951)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3129..3362
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3440..3561
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3477..3496)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3531..3550
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3601..3736
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3756..3775)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3757..3775)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3785..3802)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3785..3801)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3794..3816)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3943..4398
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4030..4049)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4240..4261
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4424..4528
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4529..4597,4598..5389)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4747..4766)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5560..6148
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6049..6068
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6302..6319
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6436..6457
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6472..6477,6478..6495,6496..6502)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6510..6526
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6515..6537
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6534..6550
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6534..6550
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6569..6589
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6571..6589
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6617..6843
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6844..7024
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7071..7196
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7689..7922
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8107..8151
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8382..8592
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8404..8600
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8451..8586
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8628..9422
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8628..9422
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8682..8701)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9292..9311
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    9470..9587
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctac
     1321 aaaatgtggc agctctgggc ctccctctgc tgcctgctgg tgttggccaa tgcccggagc
     1381 aggccctctt tccatccctt aagcgatgag ctggtcaact atgtcaacaa acggaatacc
     1441 acgtggcagg ccgggcacaa cttctacaac gtggacatga gctacttgaa gaggctatgt
     1501 ggtaccttcc tgggtgggcc caagccaccc cagagagtta tgtttaccga ggacctgaag
     1561 ctgcctgcaa gcttcgatgc acgggaacaa tggccacagt gtcccaccat caaagagatc
     1621 agagaccagg gctcctgtgg cagctgctgg gccttcgggg ctgtggaagc catctctgac
     1681 cggatctgca tccacaccaa tgcgcacgtc agcgtggagg tgtcggcgga ggacctgctc
     1741 acctgctgtg gcagcatgtg tggggacggc tgtaatggtg gctatcctgc tgaagcttgg
     1801 aacttctgga caagaaaagg cctggtttct ggtggcctct atgaatccca tgtagggtgc
     1861 agaccgtact ccatccctcc ctgtgagcac cacgtcaacg gctcccggcc cccatgcacg
     1921 ggggagggag atacccccaa gtgtagcaag atctgtgagc ctggctacag cccgacctac
     1981 aaacaggaca agcactacgg atacaattcc tacagcgtct ccaatagcga gaaggacatc
     2041 atggccgaga tctacaaaaa cggccccgtg gagggagctt tctctgtgta ttcggacttc
     2101 ctgctctaca agtcaggagt gtaccaacac gtcaccggag agatgatggg tggccatgcc
     2161 atccgcatcc tgggctgggg agtggagaat ggcacaccct actggctggt tgccaactcc
     2221 tggaacactg actggggtga caatggcttc tttaaaatac tcagaggaca ggatcactgt
     2281 ggaatcgaat cagaagtggt ggctggaatt ccacgcaccg atcagtactg ggaaaagatc
     2341 taaaacccag ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
     2401 ctcggtctcg attctacgta gtaatgaact agtaccggtt aagtcgacaa tcaacgcgtt
     2461 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     2521 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     2581 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     2641 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     2701 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     2761 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     2821 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     2881 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     2941 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     3001 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc
     3061 gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     3121 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     3181 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     3241 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     3301 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     3361 cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     3421 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     3481 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     3541 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     3601 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     3661 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     3721 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     3781 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     3841 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     3901 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     3961 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     4021 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4081 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4141 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4201 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4261 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     4321 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     4381 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4441 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4501 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     4561 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     4621 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     4681 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     4741 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     4801 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     4861 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     4921 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     4981 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     5041 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     5101 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     5161 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     5221 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     5281 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     5341 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     5401 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     5461 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     5521 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5581 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5641 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5701 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5761 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5821 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5881 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5941 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6001 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     6061 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     6121 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     6181 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     6241 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     6301 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     6361 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     6421 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     6481 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     6541 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     6601 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
     6661 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
     6721 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
     6781 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata
     6841 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     7081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     7141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     7201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     7261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     7321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     7381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     7441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     7501 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     7561 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     7621 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     7681 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     7741 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     7801 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     7861 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     7921 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     7981 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     8041 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     8101 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     8161 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     8221 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     8281 tcaccattat cgtttcagac ccacctccca accccgaggg gacccatgca ttgcatctca
     8341 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     8401 gcatgcgtct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     8461 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     8521 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     8581 gaggcctagg cttttgcaaa aagctttcta gaggtaccgt ggccaccatg attgaacaag
     8641 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     8701 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     8761 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
     8821 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     8881 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     8941 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     9001 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     9061 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     9121 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
     9181 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     9241 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     9301 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     9361 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     9421 gagttaacga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
     9481 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     9541 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     9601 gggacagcag agatccactt tggcgccgg
//
