LOCUS       sequence-30816-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Ayed A, Mulder FA, Yi GS, Lu Y, Kay LE, Arrowsmith CH
  TITLE     Latent and active p53 are identical in conformation.
  JOURNAL   Nat Struct Biol. 2001 Sep . 8(9):756-60.
  PUBMED    11524676
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Biol. 2001 Sep . 8(9):756-60."
COMMENT     Sequence Label: human p53-(310-360)
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(303..321)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      317..364
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(539..544,545..561,562..567))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(594..612)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        680..785
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aatattttgt ttactttaag aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcctggtgcc gcgcggcagc catatgaaca acaccagctc ctctccccag
      121 ccaaagaaga aaccactgga tggagaatat ttcacccttc agatccgtgg gcgtgagcgc
      181 ttcgagatgt tccgagagct gaatgaggcc ttggaactca aggatgccca ggctgggaag
      241 gagccagggt gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
      301 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
      361 tttgctgaaa ggaggaacta tatccggata tcccgcaaga ggcccggcag taccggcata
      421 accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg
      481 ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat
      541 taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt
      601 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
      661 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      721 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaagga
      781 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      841 ttcctgtttt tgctcaccca gaaacgctgg tgaaaagtaa aagatgctga agatcagttg
      901 ggtgcacgag tgggttacat cgaaactgga tctcaacagc ggtaagatcc ttga
//
