LOCUS       sequence-308163-       10834 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive lentiviral expression of human ACE2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10834)
  AUTHORS   Chavez Lab
  TITLE     Coronavirus host receptors and proteases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLEX307-ACE2-puro
FEATURES             Location/Qualifiers
     source          1..10834
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3743..3767)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3775..3816
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3867..4455
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3920..3940)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4338..4349)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4527..4760
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4838..4959
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4875..4894)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4929..4948
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4999..5134
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5154..5173)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5155..5173)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5183..5200)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5183..5199)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5192..5214)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5341..5796
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5428..5447)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5638..5659
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5822..5926
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5927..5995,5996..6787)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6145..6164)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6958..7546
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7447..7466
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7700..7717
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7834..7855
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7870..7875,7876..7893,7894..7900)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7908..7924
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7913..7935
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7932..7948
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7932..7948
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7967..7987
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7969..7987
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8015..8241
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8242..8422
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8469..8594
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9087..9320
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9505..9549
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9780..9990
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9802..9998
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9849..9984
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10030..10632
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10030..10049)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10526..10546
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10675..10792
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctac
     1321 aaaatgtcaa gctcttcctg gctccttctc agccttgttg ctgtaactgc tgctcagtcc
     1381 accattgagg aacaggccaa gacatttttg gacaagttta accacgaagc cgaagacctg
     1441 ttctatcaaa gttcacttgc ttcttggaat tataacacca atattactga agagaatgtc
     1501 caaaacatga ataatgctgg ggacaaatgg tctgcctttt taaaggaaca gtccacactt
     1561 gcccaaatgt atccactaca agaaattcag aatctcacag tcaagcttca gctgcaggct
     1621 cttcagcaaa atgggtcttc agtgctctca gaagacaaga gcaaacggtt gaacacaatt
     1681 ctaaatacaa tgagcaccat ctacagtact ggaaaagttt gtaacccaga taatccacaa
     1741 gaatgcttat tacttgaacc aggtttgaat gaaataatgg caaacagttt agactacaat
     1801 gagaggctct gggcttggga aagctggaga tctgaggtcg gcaagcagct gaggccatta
     1861 tatgaagagt atgtggtctt gaaaaatgag atggcaagag caaatcatta tgaggactat
     1921 ggggattatt ggagaggaga ctatgaagta aatggggtag atggctatga ctacagccgc
     1981 ggccagttga ttgaagatgt ggaacatacc tttgaagaga ttaaaccatt atatgaacat
     2041 cttcatgcct atgtgagggc aaagttgatg aatgcctatc cttcctatat cagtccaatt
     2101 ggatgcctcc ctgctcattt gcttggtgat atgtggggta gattttggac aaatctgtac
     2161 tctttgacag ttccctttgg acagaaacca aacatagatg ttactgatgc aatggtggac
     2221 caggcctggg atgcacagag aatattcaag gaggccgaga agttctttgt atctgttggt
     2281 cttcctaata tgactcaagg attctgggaa aattccatgc taacggaccc aggaaatgtt
     2341 cagaaagcag tctgccatcc cacagcttgg gacctgggga agggcgactt caggatcctt
     2401 atgtgcacaa aggtgacaat ggacgacttc ctgacagctc atcatgagat ggggcatatc
     2461 cagtatgata tggcatatgc tgcacaacct tttctgctaa gaaatggagc taatgaagga
     2521 ttccatgaag ctgttgggga aatcatgtca ctttctgcag ccacacctaa gcatttaaaa
     2581 tccattggtc ttctgtcacc cgattttcaa gaagacaatg aaacagaaat aaacttcctg
     2641 ctcaaacaag cactcacgat tgttgggact ctgccattta cttacatgtt agagaagtgg
     2701 aggtggatgg tctttaaagg ggaaattccc aaagaccagt ggatgaaaaa gtggtgggag
     2761 atgaagcgag agatagttgg ggtggtggaa cctgtgcccc atgatgaaac atactgtgac
     2821 cccgcatctc tgttccatgt ttctaatgat tactcattca ttcgatatta cacaaggacc
     2881 ctttaccaat tccagtttca agaagcactt tgtcaagcag ctaaacatga aggccctctg
     2941 cacaaatgtg acatctcaaa ctctacagaa gctggacaga aactgttcaa tatgctgagg
     3001 cttggaaaat cagaaccctg gaccctagca ttggaaaatg ttgtaggagc aaagaacatg
     3061 aatgtaaggc cactgctcaa ctactttgag cccttattta cctggctgaa agaccagaac
     3121 aagaattctt ttgtgggatg gagtaccgac tggagtccat atgcagacca aagcatcaaa
     3181 gtgaggataa gcctaaaatc agctcttgga gataaagcat atgaatggaa cgacaatgaa
     3241 atgtacctgt tccgatcatc tgttgcatat gctatgaggc agtacttttt aaaagtaaaa
     3301 aatcagatga ttctttttgg ggaggaggat gtgcgagtgg ctaatttgaa accaagaatc
     3361 tcctttaatt tctttgtcac tgcacctaaa aatgtgtctg atatcattcc tagaactgaa
     3421 gttgaaaagg ccatcaggat gtcccggagc cgtatcaatg atgctttccg tctgaatgac
     3481 aacagcctag agtttctggg gatacagcca acacttggac ctcctaacca gccccctgtt
     3541 tccatatggc tgattgtttt tggagttgtg atgggagtga tagtggttgg cattgtcatc
     3601 ctgatcttca ctgggatcag agatcggaag aagaaaaata aagcaagaag tggagaaaat
     3661 ccttatgcct ccatcgatat tagcaaagga gaaaataatc caggattcca aaacactgat
     3721 gatgttcaga cctcctttta gaacccagct ttcttgtaca aagtggttga tatcggtaag
     3781 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag taccggttaa
     3841 gtcgacaatc aacgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     3901 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     3961 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     4021 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     4081 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     4141 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     4201 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     4261 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     4321 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     4381 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     4441 gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag
     4501 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     4561 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     4621 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     4681 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     4741 agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt
     4801 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     4861 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     4921 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     4981 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5041 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     5101 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     5161 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     5221 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     5281 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     5341 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     5401 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     5461 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     5521 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     5581 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     5641 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     5701 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     5761 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     5821 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5881 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5941 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6001 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6061 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6121 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     6181 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     6241 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     6301 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     6361 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6421 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6481 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6541 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6601 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6661 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6721 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6781 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6841 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6901 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6961 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7021 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7081 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     7141 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7201 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7261 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7321 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7381 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7441 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7501 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7561 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7621 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     7681 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     7741 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     7801 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     7861 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     7921 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     7981 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
     8041 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     8101 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
     8161 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
     8221 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc
     8281 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     8341 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     8401 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     8461 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     8521 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     8581 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     8641 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     8701 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     8761 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     8821 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     8881 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     8941 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     9001 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     9061 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     9121 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     9181 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     9241 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     9301 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     9361 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     9421 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     9481 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     9541 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     9601 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     9661 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     9721 cccatgcatt gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     9781 caggcagaag tatgcaaagc atgcgtctca attagtcagc aaccatagtc ccgcccctaa
     9841 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     9901 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     9961 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctttctaga ggtaccaccg
    10021 gagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
    10081 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
    10141 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
    10201 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc
    10261 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
    10321 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
    10381 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
    10441 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
    10501 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
    10561 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
    10621 ggtgccagtt aacgaattct agatcttgag acaaatggca gtattcatcc acaattttaa
    10681 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
    10741 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
    10801 ttacagggac agcagagatc cactttggcg ccgg
//
