LOCUS       sequence-308203-        9021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insert: PlexAtet-citrine-tCyc1. The fluorescent protein Citrine is 
            inducible by LexA-ER-B112 upon addition of estradiol, and 
            repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9021)
  AUTHORS   Lormeau C, Rudolf F, Stelling J
  TITLE     A rationally engineered decoder of transient intracellular signals.
  JOURNAL   Nat Commun. 2021 Mar 25;12(1):1886. doi: 10.1038/s41467-021-22190-4.
  PUBMED    33767179
REFERENCE   2  (bases 1 to 9021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-25"; volume: "12(1):1886. doi"; pages: " 
            10.1038/s41467-021-22190-4"
COMMENT     Sequence Label: pCL12-decoder
FEATURES             Location/Qualifiers
     source          1..9021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(16..604)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(96..115)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(783..1574,1575..1643))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1406..1425
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1644..1748)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(2145..6323)
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     promoter        complement(6324..6563)
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     primer_bind     6665..6687
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6679..6696
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6680..6696
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6703..6722
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6703..6721
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7244..7960
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MRSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     terminator      8001..8250
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(8017..8035)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(8413..8431)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8452..8468)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8452..8468)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8465..8487)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8476..8492
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(8866..8883)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
       61 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      121 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      181 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      241 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      301 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      361 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      421 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      481 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      541 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      601 tcaagaagat cctttgatct tttgtttaaa cctacggggt ctgacgctca gtggaacgaa
      661 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
      721 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
      781 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
      841 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
      901 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
      961 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1021 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1081 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     1141 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     1201 gcggttagct ccttcggtcc tccgatggtt gtcagaagta agttggccgc agtgttatca
     1261 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     1321 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     1381 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     1441 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     1501 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     1561 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     1621 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     1681 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     1741 gttccgcgca catttccccg aaaagtgcca cctgacgtcg aaagaacgcg gcattagcaa
     1801 catttgcgaa gcgaacgatt ttgcgaactg ctattaatta attggcgcgc ctgtgaagat
     1861 cacaaggttt tgcattgagg atagtataga agcaagaatc attgaattac aggaaaaaaa
     1921 ggcaaatatg attcatgcta caataaacca agatgaagct gccattagca gactaacgcc
     1981 agccgattta cagttcttat tcaataacta atattttatt ctcttattat atattattct
     2041 cggagttttt aagtgacatc acccgaaaag aagctaagtc tttctcctaa ttcatattta
     2101 attattgtac atggacatat gcatatcata cgtaatgctc aaccttaagc tgctgcggag
     2161 cttccacgag caccagcacc tgaagcaact agacttattt gcgcttgagt tagttctata
     2221 cttggcagtg gaagtttgtc attatgagtt ggtggaggta aaaatccaac cttgtttaaa
     2281 aatgcaatat atataccaac ctcttctgga gtaacaccta ttccattaga ccaatcaaca
     2341 cctgtccatg cggtgtcttt ctttaaagat gccacggcgt tcctatcgtc tagttccgga
     2401 gctttggtac tttcaggtaa gttgtctaag accatgtgta gcaaaggata caacgcattt
     2461 tcttcattcc tgtcaataac agacgcctcc aatgatttct tccatttaga atagctttcg
     2521 atttcgacat cgtaaccata atcgtgtaaa gtatacaagt agtctttgaa taatattctt
     2581 gggtgacccg ttacttgagc aacggccaat tcattttctt tgggaggatt caaagacgta
     2641 gcaacaacaa cacgagcaac atgatctact ggaaccatat tcacggaatt ttcgatgtct
     2701 ggaatcttac ctaattggac tgaacctttc aaaaatctca ataagaaatc atctgtgttt
     2761 gaagaaccat tggcagaggc acctgttacg taacctggtc tgacaataca cccacgtagg
     2821 cccctttcac ctgcacgtct aatgatgtac tcagcagccc atttggactg accatatcca
     2881 ccagtgagcc cgcttgcaga gttcattaaa tcgtctgatt ctaaaatgcc tggcttccct
     2941 tcgctaacaa gtttatctga caaattaaag tagtattcag tgtcaagagt agaagtggag
     3001 gaaacaaagt caaagaactt tggcttgccg acggcggcta agctcataac attgatagtt
     3061 gaaataacat ttggatctct caatttggcg tatggataaa cccagtgaac taacgcacca
     3121 ttatggataa ttatatcaac tgtgtttgcc aaatccatcc acttctcatc tgaaagacca
     3181 aattggcttt tagataaatc gcctaataca actttaatat ttgaggcaaa tttttcgttc
     3241 caagtaccat aggtgatacc tgccttttgt aatcttgcaa atgcagcttc ttcatccttg
     3301 gccctgacgt gggcaaacac tttgaaactg tagttctttg gagaacgtcc taacaaatct
     3361 gcaaggatgt aggagcccag aaatcctgtg acaccagtaa caaacacatt aattgttgtt
     3421 tttccttcgg cactattagg ttcaacaaaa tattcacgag aggggtacga acttggtagc
     3481 gtctcaacca atttcttggc gtcttccgca taatttgcag tgacattttc gacgacctca
     3541 ccttgagatg atccacccga tgatttaatt ctgtcaattt ccgcggcaaa ggcctttatc
     3601 gttggatact tgaaaattgt gcccaatggt aaatcaactt gcagcttttt ctttaaggta
     3661 aaaatcattt tggtagccaa gatagaatga ccacctaaat cgaaaaacga atcatctggt
     3721 gatacagatg ctggcttggt aggtaatata cttaaccata agtctctaac ctcgcgctca
     3781 acattggtaa actgagagtc gtcagtttca gaaactgtat tttcagctac caaatttaat
     3841 tgcttgggag ttgggaattg aagtttaggc ttatcaactt taccatttgg attcaatggt
     3901 agtttatcca taaccacaat caaggaaggc atagcatagc tagccaatct tttctttaag
     3961 aaagtcctga tgtccttgga taaaagatgg taaccgatta agcccttaac tatagggtca
     4021 gtttcaacct cctttggaac atcactttgg aacttagaca agtcatctgg cttgtcaaat
     4081 cttgggacca taaatgtgat caatgttggc tcattgtcgg catttttgcg aactaaagta
     4141 atgttttctc ttaccaatgg atgttgggaa atgtgcgtat ctatttctcc taattcgatt
     4201 ctgaacccac gaattttaac ttgatcatca gccctaccgc aacattcaca gtcaccgttt
     4261 ggtagataac gacctaaatc acccgttctg tacaatctat ctcttggacc taaccagaat
     4321 tgtctccaag gttcaccatt atccttatcc aaataattcc agtgatcttt ttcaacaaac
     4381 cagttgttca caaatttttc tttattcaat tctggtaatc ctctataacc ttcggccaaa
     4441 ccacctgcac gaacataaat ctcacctatt tcgccaatac cacatatttg agtacggtcg
     4501 ttcctgttaa caactaatag ctgaacgttc aacatacctt taccagcagg catgacatct
     4561 ttcaattttt tcaaaaagtt tggatcgtca ttttttgatt taacttcgaa ataagaaact
     4621 gcacgctgtg tttcagtagt accgtacata ttaacaatac ggcaattttc tgccaaggtt
     4681 tgtaacctca gacaatcacg ttttgttaaa atgtcaccca caaagaacgc atgatgtaac
     4741 ttagggaatg gtgtagtagc ttgggcagta agtaattgac ccatggcagg tgttaaatgg
     4801 gtaactgtgc aaccatactt actcatccat tccgctaaac ggcccggtgt accaatatca
     4861 tcttgagtag ggacatacaa ttgggcacct aaaaataatg gtgtaaacat atctctttga
     4921 attggatcat gtgcaatacc gctcagcatt gtgaatttat cattttctgt taagttgaac
     4981 cttttggaca tccaattgaa ataataagcc aaggaaaaat gtctaccaag aacaccctta
     5041 ggaatacctt cggaaccaga tgtgaaagat agggttgggt tggaatctgg tccaactaca
     5101 acacctgttc tggtgtcttt gtagtgatcg tatggagcca aaacatcctc gccattgtcc
     5161 aatttgccac cttcaatagt accattttct tgaatagcga tggaattgat tcttgaaaca
     5221 atctccaatt catcattgat gtaatcttct actaattgat ccaattgtcc agcagctcta
     5281 ataacaatca acccacgtgg tttagcaaca cctaagtaaa tggtttgtct ggctggggga
     5341 tatgcagggt cgataactga aaaggttgcg ccagctttca agacacccat cacacatacc
     5401 atcaaatcca cacccctaga agaatagatc atcactacat cacctctttt gatacctgtt
     5461 ttaatcaaat aatgggcaac tatgttagaa gtgcggttga tgtcgcgata agtgaaagaa
     5521 cgggacttgt cggaatttag tgttggagtc tccacaacac aggttctctc tgggaaggct
     5581 tcagcattgt cctggaaaat gtcgtgaata caccccacga aatcgcacca gcccaagttc
     5641 ttagttggat caggtaaact atccttggat gatgcggtga tcagagagat tttagttatg
     5701 caattggatg gatcgcttag cgcagcagtc aaatattgag taaattggtc cgcaacaatg
     5761 gttactcttt cattcgaata cagtaagctg ttataaatta agttcaaaac atgcgcatta
     5821 ttactggtat ccaaattcaa agcaaagtcc actaaatgat gcttgaactc gtctaatttg
     5881 aaatcttggt tttccaaaaa ggccaaacgg aacaactgag gggtcctttc caagtcttgg
     5941 caactttgaa ttttttcagc tagctcgtca aaggaaaaat tggcctcaat agaattgagc
     6001 ttgttcaact cattgttaat tgtagaatac agctcattaa atgaccacgt tggttgaata
     6061 ttgaatctta agattttgtt attcgcaata taaagaacaa tatcatcgtc accagttact
     6121 ctatatatca atgcagccca tacactcaaa gcgacagcgt atttgttaga aaaactatca
     6181 tgaggcacat cgagctgagg tagctgtaac gaatatgtag cttgtttcgt ataaggttct
     6241 tgttgtgggc gtaaaaagtc atgtggtaac actgaaagag ttggattatc caacttctct
     6301 atccagacct tttcgttagt cattagtgag taactctgtg atatgtctct ataattagca
     6361 gtttttcact gaaattccag gaaaggtaat aaactcagat ttttttttat actattggct
     6421 gcttgttact tatatatctt gaacttctcc cagcgggtct tcaaatacat ttgggcgatg
     6481 ttcatgttca ttaggcaggt atttcgacat tgagtcacac gcgaaaaacc gccggaattt
     6541 tttatgtaat tgcaagtgca attccgctgg caaaactatt gaagagtttt cctcgctaaa
     6601 actgtgcgat gcctctcgat cggatgtgct gcaaggcgat taagttgggt aacgccaggg
     6661 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactatag
     6721 ggcgaattgg agaaacagaa taatatataa acctgtataa tataaccttg aagactatat
     6781 ttcttttcgc ggccgcctat agggcgaatt ggagctccct aggtgctgta tatactcaca
     6841 gcataactgt atatacaccc agggtctagg tgctgtatat actcacagca taactgtata
     6901 tacacccagg gtctaggtgc tgtatatact cacagcataa ctgtatatac acccagggtc
     6961 taggtgctgt atatactcac agcataactg tatatacacc cagggtctag aaaaggttga
     7021 aaccagttcc ctgaaattat tcccctatct atcattgata gatataaaag tctatcattg
     7081 atagagtaat tctgtaaatc tatttcttaa acttcttaaa ttctactttt atagttagtc
     7141 ttttttttag ttttaaaaca ccaagaactt agtttcgaat aaacacacat aaacaaacaa
     7201 aactagtgga tcccccgggc tgcaggaatt cgatatcaag cttatgagat ctaaaggtga
     7261 agaattattc actggtgttg tcccaatttt ggttgaatta gatggtgatg ttaatggtca
     7321 caaattttct gtctccggtg aaggtgaagg tgatgctact tacggtaaat tgaccttaaa
     7381 atttatttgt actactggta aattgccagt tccatggcca accttagtca ctactttagg
     7441 ttatggtttg atgtgttttg ctagataccc agatcatatg aaacaacatg actttttcaa
     7501 gtctgccatg ccagaaggtt atgttcaaga aagaactatt tttttcaaag atgacggtaa
     7561 ctacaagacc agagctgaag tcaagtttga aggtgatacc ttagttaata gaatcgaatt
     7621 aaaaggtatt gattttaaag aagatggtaa cattttaggt cacaaattgg aatacaacta
     7681 taactctcac aatgtttaca tcatggctga caaacaaaag aatggtatca aagttaactt
     7741 caaaattaga cacaacattg aagatggttc tgttcaatta gctgaccatt atcaacaaaa
     7801 tactccaatt ggtgatggtc cagtcttgtt accagacaac cattacttat cctatcaatc
     7861 taagttatcc aaagatccaa acgaaaagag agaccacatg gtcttgttag aatttgttac
     7921 tgctgctggt attacccatg gtatggatga attgtacaaa ggatcctaac tcgagacagg
     7981 ccccttttcc tttgtctata tcatgtaatt agttatgtca cgcttacatt cacgccctcc
     8041 tcccacatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat
     8101 ttattttttt taatagttat gttagtatta agaacgttat ttatatttca aatttttctt
     8161 ttttttctgt acaaacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag
     8221 gttttgggac gctcgaaggc tttaatttgc aaggttcgca gtttacactc tcatcgtcgc
     8281 tctcatcatc gcttccgttg ttgttttcct tagtagcgtc tgcttggtac ccagcttcgg
     8341 ccgtatttac agataacgat gtcgttatta tatatatata tatatattgt cacttgtacc
     8401 cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat ggtcatagct
     8461 gtttcctgtg tgaaattgtt atccgctcac aacagctgag aagcggtcag caagaagaaa
     8521 atcgaataat cagtttcaat gaatatagac gtaacaaagg cacaaaaaag aaaacaagta
     8581 gatatgtcat acatatatat atatttcagg cagcaagtga ccatcaacca ggaagtgtga
     8641 aatagcaaag aactgtttac ttcttcagac ttagaaagct cttcccatag gagaaagcaa
     8701 ctgctttcct ttgtagtaat tgattgtctt ttcagccctt gacgcaacac cgtccgctgt
     8761 gaaatcgaac aatttgtaaa tttcaggcgc gccatcctgc aggcattaat gaatcggcca
     8821 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8881 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8941 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9001 ggccaggaac cgtaaaaagg c
//
