LOCUS       sequence-30822-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Sheng Y, Laister RC, Lemak A, Wu B, Tai E, Duan S, Lukin J, 
            Sunnerhagen M, Srisailam S, Karra M, Benchimol S, Arrowsmith CH
  TITLE     Molecular basis of Pirh2-mediated p53 ubiquitylation.
  JOURNAL   Nat Struct Mol Biol. 2008 Dec . 15(12):1334-42.
  PUBMED    19043414
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2008 Dec . 15(12):1334-42."
COMMENT     Sequence Label: human pirh2-(187-261)
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..31
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(334..356)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(501..519)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        587..691
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(911..930)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 catctcaatc ggactggttc cgcgtggatc tcatatgatg cactctgctt tagatatgac
       61 caggtattgg agacagctgg atgatgaagt agcacagact cctatgccat cagaatatca
      121 gaacatgact gtggatattc tctgcaatga ctgtaatgga cgatccactg ttcagtttca
      181 tatattaggc atgaaatgta agatttgtga atcctataat actgctcaag ctggaggacg
      241 tagaatttca ctggatcagc aatgaggatc cccgggaatt catcgtgact gactgacgat
      301 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
      361 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
      421 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
      481 taattcctga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
      541 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      601 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      661 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      721 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      781 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      841 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      901 ttaaagttct gctatgtggc gcggtattat cccgtgt
//
