LOCUS       sequence-308222-        8379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-RAM, the Npas4-dependent reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Sun X, Bernstein MJ, Meng M, Rao S, Sorensen AT, Yao L, Zhang X, 
            Anikeeva PO, Lin Y
  TITLE     Functionally Distinct Neuronal Ensembles within the Memory Engram.
  JOURNAL   Cell. 2020 Apr 16;181(2):410-423.e17. doi: 
            10.1016/j.cell.2020.02.055. Epub 2020 Mar 17.
  PUBMED    32187527
REFERENCE   2  (bases 1 to 8379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.02.055"; journalName: "Cell"; date: 
            "2020-04-16- 16"; volume: "181"; issue: "2"; pages: "410-423.e17"
COMMENT     Sequence Label: pAAV-N-RAM-d2tTA-TRE-mKate2
FEATURES             Location/Qualifiers
     source          1..8379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..455
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     297..318
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        481..585
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(586..654,655..1446)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(804..823)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1617..2205
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2106..2125
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  2510..2734
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2801..3334)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3504..3509,3510..3526,3527..3532))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     repeat_region   3872..4001
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        4021..4335
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    4029..4047
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4065..4083
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4100..4118
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4136..4154
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4172..4190
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4207..4225
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4243..4261
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4308..4332
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4362..4371
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4368..5066
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     misc_feature    5119..5707
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5172..5192)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5590..5601)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5740..5757)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5746..5857
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    6005..6053
                     /note="synthetic polyadenylation signal"
     misc_feature    6067..6158
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     6107..6126
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     CDS             join(6553..7170,7171..7176,7177..7212,7213..7215,7216..7251,
                     7252..7254,7255..7290,7291..7299)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(7312..7328)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7353..7372)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7354..7372)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    7398..7532
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   7561..7701
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   7561..7690
                     /label=AAV2 ITR
     polyA_signal    7868..8002
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  8031..8196
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc ttaatagtgg
      301 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
      361 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
      421 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
      481 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
      541 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      601 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      661 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      721 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      781 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      841 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      901 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      961 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     1021 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     1081 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     1141 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     1201 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     1261 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     1321 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     1381 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     1441 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1501 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1561 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1621 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1681 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1741 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1801 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1861 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1921 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1981 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2041 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2101 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2161 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2221 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2281 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2341 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2401 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tagaccagcc gcgtaacctg
     2461 gcaaaatcgg ttacggttga gtaataaatg gatgccctgc gtaagcgggt gtgggcggac
     2521 aataaagtct taaactgaac aaaatagatc taaactatga caataaagtc ttaaactaga
     2581 cagaatagtt gtaaactgaa atcagtccag ttatgctgtg aaaaagcata ctggactttt
     2641 gttatggcta aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg tattaaagag
     2701 gggcgtggcc aagggcatgg taaagactat attcgcggcg ttgtgacaat ttaccgaaca
     2761 actccgcggc cgggaagccg atctcggctt gaacgaattg ttaggtggcg gtacttgggt
     2821 cgatatcaaa gtgcatcact tcttcccgta tgcccaactt tgtatagaga gccactgcgg
     2881 gatcgtcacc gtaatctgct tgcacgtaga tcacataagc accaagcgcg ttggcctcat
     2941 gcttgaggag attgatgagc gcggtggcaa tgccctgcct ccggtgctcg ccggagactg
     3001 cgagatcata gatatagatc tcactacgcg gctgctcaaa cttgggcaga acgtaagccg
     3061 cgagagcgcc aacaaccgct tcttggtcga aggcagcaag cgcgatgaat gtcttactac
     3121 ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg gctacgtctc
     3181 cgaactcacg accgaaaaga tcaagagcag cccgcatgga tttgacttgg tcagggccga
     3241 gcctacatgt gcgaatgatg cccatacttg agccacctaa ctttgtttta gggcgactgc
     3301 cctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3361 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3421 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3481 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3541 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3601 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3661 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc
     3721 aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3781 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3841 atagttctag tggttggcta cagcttgcat gcctgcaggc agctgcgcgc tcgctcgctc
     3901 actgaggccg cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc
     3961 gcgcagagag ggagtggcca actccatcac taggggttcc tgcggccgca cgcgcgtctc
     4021 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
     4081 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
     4141 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
     4201 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
     4261 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     4321 aaccgtcaga tcgccaccgg tgtttaaacg ctagcgcggc cgccaccatg gtgagcgagc
     4381 tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac aaccaccact
     4441 tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca
     4501 aggcggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt
     4561 acggcagcaa aaccttcatc aaccacaccc agggcatccc cgacttcttt aagcagtcct
     4621 tccccgaggg cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg
     4681 ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg
     4741 tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggcctcca
     4801 ccgagaccct gtaccccgct gacggcggcc tggaaggcag agccgacatg gccctgaagc
     4861 tcgtgggcgg gggccacctg atctgcaact tgaagaccac atacagatcc aagaaacccg
     4921 ctaagaacct caagatgccc ggcgtctact atgtggacag aagactggaa agaatcaagg
     4981 aggccgacaa agagacctac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc
     5041 tccctagcaa actggggcac agatgagtcg acggcgcgcc agcgatccac cggatctaga
     5101 taactgatcc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     5161 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     5221 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     5281 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     5341 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     5401 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     5461 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     5521 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gcccttctgc
     5581 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     5641 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     5701 tccccgcatc gataccgtcg actagagctc gctgatcagc ctcgactgtg ccttctagtt
     5761 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     5821 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     5881 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     5941 gactagtacg tctctcaagg ataagtaagt aatattaagg tacgggaggt attggacagg
     6001 ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat
     6061 agtactaaca tacgctctcc atcaaaacaa aacgaaacaa aacaaactag caaaataggc
     6121 tgtccccagt gcaagtgcag gtgccagaac atttctctgg cctaactggc cgacgcgtcc
     6181 taactggccg gtaccctaga agtttgttcg tgactgtgac tagaagtttg ttcgtgactg
     6241 tgactagaag tttgttcgtg actgtgacta gaagtttgtt cgtgactgtg acatgcatca
     6301 ttcataaaac gcttgttata aaagcagtgg ctgcggcgcc tcgtactcca accgcatctg
     6361 cagcgagcaa ctgagaagcc aagactgagc cggcggccga gctcgctagg gatccagctc
     6421 atgagccatg gcttcccgcc ggaggtggag gagcaggatg atggcacgct gcccatgtct
     6481 tgtgcccagg agagcgggat ggaccgtcac cctgcagcct gtgcttctgc taggatcaat
     6541 gtggaattca ccatgtctag actggacaag agcaaagtca taaactctgc tctggaatta
     6601 ctcaatgaag tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt
     6661 gagcagccta ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctggca
     6721 atcgagatgc tggacaggca tcatacccac ttctgccccc tggaaggcga gtcatggcaa
     6781 gactttctgc ggaacaacgc caagtcattc cgctgtgctc tcctctcaca tcgcgacggg
     6841 gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag
     6901 ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc
     6961 gtgggccact ttacactggg ctgcgtattg gaggatcagg agcatcaagt agcaaaagag
     7021 gaaagagaga cacctaccac cgattctatg cccccacttc tgagacaagc aattgagctg
     7081 ttcgaccatc agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc
     7141 ctggagaaac agctaaagtg cgaaagcggc gggccggccg acgcccttga cgattttgac
     7201 ttagacatgc tcccagccga tgcccttgac gactttgacc ttgatatgct gcctgctgac
     7261 gctcttgacg attttgacct tgacatgctc cccgggtaac taagtaagca tcgataccgt
     7321 cgacctcgag ggggggcccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgca
     7381 gcggccgacc atggcccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7441 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7501 aactcatcaa tgtatcttat catgtctgga tctccggaca cgtgcggacc gagcggccgc
     7561 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
     7621 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
     7681 gagcgcgcag ctgcctgcag gtctgagaca ataaccctga taaatgcttc aataatgtaa
     7741 gcttgtcgag aagtactaga ggatcataat cagccatacc acatttgtag aggttttact
     7801 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     7861 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7921 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7981 tgtatcttat catgtctgga tctgatcact gatatcgcct aggagatccg aaccagataa
     8041 gtgaaatcta gttccaaact attttgtcat ttttaatttt cgtattagct tacgacgcta
     8101 cacccagttc ccatctattt tgtcactctt ccctaaataa tccttaaaaa ctccatttcc
     8161 acccctccca gttcccaact attttgtccg cccacagcgg ggcatttttc ttcctgttat
     8221 gtttttaatc aaacatcctg ccaactccat gtgacaaacc gtcatcttcg gctacttttt
     8281 ctctgtcaca gaatgaaaat ttttctgtca tctcttcgtt attaatgttt gtaattgact
     8341 gaatatcaac gcttatttgc agcctgaatg gcgaatggg
//
