LOCUS       sequence-30823-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Irwin M, Marin MC, Phillips AC, Seelan RS, Smith DI, Liu W, Flores 
            ER, Tsai KY, Jacks T, Vousden KH, Kaelin WG
  TITLE     Role for the p53 homologue p73 in E2F-1-induced apoptosis.
  JOURNAL   Nature. 2000 Oct 5. 407(6804):645-8.
  PUBMED    11034215
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2000 Oct 5. 407(6804):645-8."
COMMENT     Sequence Label: T7-p53DD-pcDNA3
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      79..88
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(538..556)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(539..556)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(576..593)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    582..806
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgc caccatggct agcatgactg gtggacagca aatgggatcc
      121 cccccaggga gcactaagcg agcactgccc aacaacacca gctcctctcc ccagccaaag
      181 aagaaaccac tggatggaga atatttcacc cttcagatcc gtgggcgtga gcgcttcgag
      241 atgttccgag agctgaatga ggccttggaa ctcaaggatg cccaggctgg gaaggagcca
      301 ggggggagca gggctcactc cagccacctg aagtccaaaa agggtcagtc tacctcccgc
      361 cataaaaaac acatgttcaa gacagaaggg cctgactcag actgagaatt cccaattgca
      421 cctgaaattg cactggaact gctgatggct gcgaacttct tagattgtta aggtctccaa
      481 ttctgcagat atccatcaca ctggcggccg ctcgagcatg catctagagg gccctattct
      541 atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
      601 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
      661 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
      721 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
      781 tgctggggat gcggtgggct ctatggcttc tga
//
