LOCUS       sequence-30824-e         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Yang Q, Manicone A, Coursen JD, Linke SP, Nagashima M, Forgues M, 
            Wang XW
  TITLE     Identification of a functional domain in a GADD45-mediated G2/M 
            checkpoint.
  JOURNAL   J Biol Chem. 2000 Nov 24. 275(47):36892-8.
  PUBMED    10973963
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Nov 24. 275(47):36892-8."
COMMENT     Sequence Label: pcDNA3-HA+WT GADD45
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..46)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(560..579)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(561..579)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(624..723)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(653..673)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctatagaata gggccctcag gcataatctg gcacatcata agggtatcta gaccgttcag
       61 ggagattaat cactggaacc cattgatcca tgtagcgact ttcccggcaa aaacaaataa
      121 gttgacttaa ggcaggatcc ttccattgag atgaatgtgg attcgtcacc agcacgcagt
      181 gcaggtccgg gggctgctcg gcgccctcgc tcgccgcggg gccagcgtcg gtctccaaga
      241 gcaggagctc cgccagccgg cccgggttgc tgacgcgcag gatgttgatg tcgttctcgc
      301 agcaaaacgc ctggatcagg gtgaagtgga tctgcagagc cacatctctg tcgtcgtcct
      361 cgtccgccgc cagcaggcac aacaccacgt tatcggggtc gacgttgagc agcttggccg
      421 cttcgtacac cccgacagtg atcgtgcgct gactcagggc tttgctgagc acttcctcca
      481 gggcatcccc caccttatcc atcctttcgg tcttctgctc tccagccgag aattcctcca
      541 aagtcataag cttgggtctc cctatagtga gtcgtattaa tttcgataag ccagtaagca
      601 gtgggttctc tagttagcca gagagctctg cttatataga cctcccaccg tacacgccta
      661 ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt
      721 tggtgccaaa a
//
