LOCUS       sequence-308250-        3552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Cloning compatible entry vector for the human CTSL gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Chavez Lab
  TITLE     Coronavirus host receptors and proteases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDONR221-CTSL
FEATURES             Location/Qualifiers
     source          1..3552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1677..1776)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1793..1812)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1794..1812)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1935..1954)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1946..2755
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2013..2032)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2902..3490
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3391..3410
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctac aaaatgaatc ctacactcat ccttgctgcc ttttgcctgg gaattgcctc
      721 agctactcta acatttgatc acagtttaga ggcacagtgg accaagtgga aggcgatgca
      781 caacagatta tacggcatga atgaagaagg atggaggaga gcagtgtggg agaagaacgt
      841 gaagatgatt gaactgcaca atcaggaata cagggaaggg aaacacagct tcacaatggc
      901 catgaacgcc tttggagaca tgaccagtga agaattcagg caggtgatga atggctttca
      961 aaaccgtaag cccaggaagg ggaaagtgtt ccaggaacct ctgttttatg aggcccccag
     1021 atctgtggat tggagagaga aaggctacgt gactcctgtg aagaatcagg gtcagtgtgg
     1081 ttcttgttgg gcttttagtg ctactggtgc tcttgaagga cagatgttcc ggaaaactgg
     1141 gaggcttatc tcactgagtg agcagaatct ggtagactgc tctgggcctc aaggcaatga
     1201 aggctgcaat ggtggcctaa tggattatgc tttccagtat gttcaggata atggaggcct
     1261 ggactctgag gaatcctatc catatgaggc aacagaagaa tcctgtaagt acaatcccaa
     1321 gtattctgtt gctaatgaca ccggctttgt ggacatccct aagcaggaga aggccctgat
     1381 gaaggcagtt gcaactgtgg ggcccatttc tgttgctatt gatgcaggtc atgagtcctt
     1441 cctgttctat aaagaaggca tttattttga gccagactgt agcagtgaag acatggatca
     1501 tggtgtgctg gtggttggct acggatttga aagcacagaa tcagataaca ataaatattg
     1561 gctggtgaag aacagctggg gtgaagaatg gggcatgggt ggctacgtaa agatggccaa
     1621 agaccggaga aaccattgtg gaattgcctc agcagccagc taccccactg tgtagaaccc
     1681 agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     1741 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     1801 tgagtcgtat tacatggtca tagctgtttc ttggcagctc tggcccgtgt ctcaaaatct
     1861 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
     1921 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg
     1981 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     2041 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
     2101 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     2161 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     2221 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc
     2281 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     2341 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
     2401 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     2461 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
     2521 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     2581 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     2641 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     2701 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     2761 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag
     2821 ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     2881 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2941 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3001 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3061 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3121 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3181 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3241 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3301 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3361 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3421 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3481 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3541 tgctcacatg tt
//
