LOCUS       sequence-308252-        3807 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Cloning compatible entry vector for the human TMPRSS11D 
            gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Chavez Lab
  TITLE     Coronavirus host receptors and proteases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDONR221-TMPRSS11D
FEATURES             Location/Qualifiers
     source          1..3807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1932..2031)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2048..2067)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2049..2067)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2072..2088)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2072..2088)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2190..2209)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2201..3010
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2268..2287)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3157..3745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3646..3665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctac aaaatgtata ggccagcacg tgtaacttcg acttcaagat ttctgaatcc
      721 atatgtagta tgtttcattg tcgtcgcagg ggtagtgatc ctggcagtca ccatagctct
      781 acttgtttac tttttagctt ttgatcaaaa atcttacttt tataggagca gttttcaact
      841 cctaaatgtt gaatataata gtcagttaaa ttcaccagct acacaggaat acaggacttt
      901 gagtggaaga attgaatctc tgattactaa aacattcaaa gaatcaaatt taagaaatca
      961 gttcatcaga gctcatgttg ccaaactgag gcaagatggt agtggtgtga gagcggatgt
     1021 tgtcatgaaa tttcaattca ctagaaataa caatggagca tcaatgaaaa gcagaattga
     1081 gtctgtttta cgacaaatgc tgaataactc tggaaacctg gaaataaacc cttcaactga
     1141 gataacatca cttactgacc aggctgcagc aaattggctt attaatgaat gtggggccgg
     1201 tccagaccta ataacattgt ctgagcagag aatccttgga ggcactgagg ctgaggaggg
     1261 aagctggccg tggcaagtca gtctgcggct caataatgcc caccactgtg gaggcagcct
     1321 gatcaataac atgtggatcc tgacagcagc tcactgcttc agaagcaact ctaatcctcg
     1381 tgactggatt gccacgtctg gtatttccac aacatttcct aaactaagaa tgagagtaag
     1441 aaatatttta attcataaca attataaatc tgcaactcat gaaaatgaca ttgcacttgt
     1501 gagacttgag aacagtgtca cctttaccaa agatatccat agtgtgtgtc tcccagctgc
     1561 tacccagaat attccacctg gctctactgc ttatgtaaca ggatggggcg ctcaagaata
     1621 tgctggccac acagttccag agctaaggca aggacaggtc agaataataa gtaatgatgt
     1681 atgtaatgca ccacatagtt ataatggagc catcttgtct ggaatgctgt gtgctggagt
     1741 acctcaaggt ggagtggacg catgtcaggg tgactctggt ggcccactag tacaagaaga
     1801 ctcacggcgg ctttggttta ttgtggggat agtaagctgg ggagatcagt gtggcctgcc
     1861 ggataagcca ggagtgtata ctcgagtgac agcctacctt gactggatta ggcaacaaac
     1921 tgggatctag aacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc
     1981 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
     2041 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     2101 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
     2161 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     2221 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     2281 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     2341 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     2401 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     2461 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     2521 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     2581 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     2641 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     2701 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     2761 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     2821 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     2881 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     2941 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     3001 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg
     3061 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     3121 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3181 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3241 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3301 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3361 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3421 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3481 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3541 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3601 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3661 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3721 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3781 cttttgctgg ccttttgctc acatgtt
//
