LOCUS       sequence-308264-       12042 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Temperature-sensitive plasmid carries tnsABCD genes that encode 
            transposase biochemical machinery under an arabinose-inducible pBAD 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12042)
  AUTHORS   Jasinska W, Manhart M, Lerner J, Gauthier L, Serohijos AWR, 
            Bershtein S
  TITLE     Chromosomal barcoding of E. coli populations reveals lineage 
            diversity dynamics at high resolution.
  JOURNAL   Nat Ecol Evol. 2020 Feb 24. pii: 10.1038/s41559-020-1103-z. doi: 
            10.1038/s41559-020-1103-z.
  PUBMED    32094541
REFERENCE   2  (bases 1 to 12042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Ecol 
            Evol. 2020 Feb 24. pii: 10.1038/s41559-020-1103-z. doi: 
            10.1038/s41559-020-1103-z."
COMMENT     Sequence Label: Tn7 helper plasmid
FEATURES             Location/Qualifiers
     source          1..12042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(6976..6993)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      7146..7232
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7324..7351
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        7370..7461
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7462..7530,7531..8322)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7680..7699)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(8451..8470)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     oriT            8493..8602
                     /note="incP origin of transfer"
     primer_bind     complement(8640..8659)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      9342..9564
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             9612..10562
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     CDS             complement(11138..12016)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg
      301 ctagcgaatt cgagctcggt acccaagtct taaactagac agaatagttg taaactgaaa
      361 tcagtccagt tatgctgtga aaaagcatac tggacttttg ttatggctaa agcaaactct
      421 tcattttctg aagtgcaaat tgcccgtcgt attaaagagg ggcgtggcca agggcatggt
      481 aaagactata ttccatggct aacagtacaa gaagttcctt cttcaggtcg ttcccaccgt
      541 atttattctc ataagacggg acgagtccat catttgctat ctgacttaga gcttgctgtt
      601 tttctcagtc ttgagtggga gagcagcgtg ctagatatac gcgagcagtt ccccttatta
      661 cctagtgata ccaggcagat tgcaatagat agtggtatta agcatcctgt tattcgtggt
      721 gtagatcagg ttatgtctac tgatttttta gtggactgca aagatggtcc ttttgagcag
      781 tttgctattc aagtcaaacc tgcagcagcc ttacaagacg agcgtacctt agaaaaacta
      841 gaactagagc gtcgctattg gcagcaaaag caaattcctt ggttcatttt tactgataaa
      901 gaaataaatc ccgtagtaaa agaaaatatt gaatggcttt attcagtgaa aacagaagaa
      961 gtttctgcgg agcttttagc acaactatcc ccattggccc atatcctgca agaaaaagga
     1021 gatgaaaaca ttatcaatgt ctgtaagcag gttgatattg cttatgattt ggagttaggc
     1081 aaaacattga gtgagatacg agccttaacc gcaaatggtt ttattaagtt caatatttat
     1141 aagtctttca gggcaaataa gtgtgcagat ctctgtatta gccaagtagt gaatatggag
     1201 gagttgcgct atgtggcaaa ttaatgaggt tgtgctattt gataatgatc cgtatcgcat
     1261 tttggctata gaggatggcc aagttgtctg gatgcaaata agcgctgata aaggagttcc
     1321 acaagctagg gctgagttgt tgctaatgca gtatttagat gaaggccgct tagttagaac
     1381 tgatgaccct tatgtacatc ttgatttaga agagccgtct gtagattctg tcagcttcca
     1441 gaagcgcgag gaggattatc gaaaaattct tcctattatt aatagtaagg atcgtttcga
     1501 ccctaaagtc agaagcgaac tcgttgagca tgtggtccaa gaacataagg ttactaaggc
     1561 tacagtttat aagttgttac gccgttactg gcagcgtggt caaacgccta atgcattaat
     1621 tcctgactac aaaaacagcg gtgcaccagg ggaaagacgt tcagcgacag gaacagcaaa
     1681 gattggccga gccagagaat atggtaaggg tgaaggaacc aaggtaacgc ccgagattga
     1741 acgccttttt aggttgacca tagaaaagca cctgttaaat caaaaaggta caaagaccac
     1801 cgttgcctat agacgatttg tggacttgtt tgctcagtat tttcctcgca ttccccaaga
     1861 ggattaccca acactacgtc agtttcgtta tttttatgat cgagaatacc ctaaagctca
     1921 gcgcttaaag tctagagtta aagcaggggt atataaaaaa gacgtacgac ccttaagtag
     1981 tacagccact tctcaggcgt taggccctgg gagtcgttat gagattgatg ccacgattgc
     2041 tgatatttat ttagtggatc atcatgatcg ccaaaaaatc ataggaagac caacgcttta
     2101 cattgtgatt gatgtgttta gtcggatgat cacgggcttt tatatcggct ttgaaaatcc
     2161 gtcttatgtg gtggcgatgc aggcttttgt aaatgcttgc tctgacaaaa cggccatttg
     2221 tgcccagcat gatattgaga ttagtagctc agactggccg tgtgtaggtt tgccagatgt
     2281 gttgctagcg gaccgtggcg aattaatgag tcatcaggtc gaagccttag tttctagttt
     2341 taatgtgcga gtggaaagtg ctccacctag acgtggcgat gctaaaggca tagtggaaag
     2401 cacttttaga acactacaag ccgagtttaa gtcctttgca cctggcattg tagagggcag
     2461 tcggatcaaa agccatggtg aaacagacta taggttagat gcatctctgt cggtatttga
     2521 gttcacacaa attattttgc gtacgatctt attcagaaat aaccatctgg tgatggataa
     2581 atacgatcga gatgctgatt ttcctacaga tttaccgtct attcctgtcc agctatggca
     2641 atggggtatg cagcatcgta caggtagttt aagggctgtg gagcaagagc agttgcgagt
     2701 agcgttactg cctcgccgaa aggtctctat ttcttcattt ggcgttaatt tgtggggttt
     2761 gtattactcg gggtcagaga ttctgcgtga gggttggttg cagcggagca ctgatatagc
     2821 tagacctcaa catttagaag cggcttatga cccagtgctg gttgatacga tttatttgtt
     2881 tccgcaagtt ggcagccgtg tattttggcg ctgtaatctg acggaacgta gtcggcagtt
     2941 taaaggtctc tcattttggg aggtttggga tatacaagca caagaaaaac acaataaagc
     3001 caatgcgaag caggatgagt taactaaacg cagggagctt gaggcgttta ttcagcaaac
     3061 cattcagaaa gcgaataagt taacgcccag tactactgag cccaaatcaa cacgcattaa
     3121 gcagattaaa actaataaaa aagaagccgt gacctcggag cgtaaaaaac gtgcggagca
     3181 tttgaagcca agctcttcag gtgatgaggc taaagttatt cctttcaacg cagtggaagc
     3241 ggatgatcaa gaagattaca gcctacccac atacgtgcct gaattatttc aggatccacc
     3301 agaaaaggat gagtcatgag tgctacccgg attcaagcag tttatcgtga tacgggggta
     3361 gaggcttatc gtgataatcc ttttatcgag gccttaccac cattacaaga gtcagtgaat
     3421 agtgctgcat cactgaaatc ctctttacag cttacttcct ctgacttgca aaagtcccgt
     3481 gttatcagag ctcataccat ttgtcgtatt ccagatgact attttcagcc attaggtacg
     3541 catttgctac taagtgagcg tatttcggtc atgattcgag gtggctacgt aggcagaaat
     3601 cctaaaacag gagatttaca aaagcattta caaaatggtt atgagcgtgt tcaaacggga
     3661 gagttggaga catttcgctt tgaggaggca cgatctacgg cacaaagctt attgttaatt
     3721 ggttgttctg gtagtgggaa gacgacctct cttcatcgta ttctagccac gtatcctcag
     3781 gtgatttacc atcgtgaact caatgtagag caggtggtgt atttgaaaat agactgctcg
     3841 cataatggtt cgctaaaaga aatctgcttg aattttttca gagcgttgga tcgagccttg
     3901 ggctcgaact atgagcgtcg ttatggctta aaacgtcatg gtatagaaac catgttggct
     3961 ttgatgtcgc aaatagccaa tgcacatgct ttagggttgt tggttattga tgaaattcag
     4021 catttaagcc gctctcgttc gggtggatct caagagatgc tgaacttttt tgtgacgatg
     4081 gtgaatatta ttggcgtacc agtgatgttg attggtaccc ctaaagcacg agagattttt
     4141 gaggctgatt tgcggtctgc acgtagaggg gcagggtttg gagctatatt ctgggatcct
     4201 atacaacaaa cgcaacgtgg aaagcccaat caagagtgga tcgcttttac ggataatctt
     4261 tggcaattac agcttttaca acgcaaagat gcgctgttat cggatgaggt ccgtgatgtg
     4321 tggtatgagc taagccaagg agtgatggac attgtagtaa aactttttgt actcgctcag
     4381 ctccgtgcgc tagctttagg caatgagcgt attaccgctg gtttattgcg gcaagtgtat
     4441 caagatgagt taaagcctgt gcaccccatg ctagaggcat tacgctcggg tatcccagaa
     4501 cgcattgctc gttattctga tctagtcgtt cccgagattg ataaacggtt aatccaactt
     4561 cagctagata tcgcagcgat acaagaacaa acaccagaag aaaaagccct tcaagagtta
     4621 gataccgaag atcagcgtca tttatatctg atgctgaaag aggattacga ttcaagcctg
     4681 ttaattccca ctattaaaaa agcgtttagc cagaatccaa cgatgacaag acaaaagtta
     4741 ctgcctcttg ttttgcagtg gttgatggaa ggcgaaacgg tagtgtcaga actagaaaag
     4801 ccctccaaga gtaaaaaggt ttcggctata aaggtagtca agcccagcga ctgggatagc
     4861 ttgcctgata cggatttacg ttatatctat tcacaacgcc aacctgaaaa aaccatgcat
     4921 gaacggttaa aagggaaagg ggtaatagtg gatatggcga gcttatttaa acaagcaggt
     4981 tagccatgag aaactttcct gttccgtact cgaatgagct gatttatagc actattgcac
     5041 gggcaggcgt ttatcaaggg attgttagtc ctaagcagct gttggatgag gtgtatggca
     5101 accgcaaggt ggtcgctacc ttaggtctgc cctcgcattt aggtgtgata gcaagacatc
     5161 tacatcaaac aggacgttac gctgttcagc agcttattta tgagcatacc ttattccctt
     5221 tatatgctcc gtttgtaggc aaggagcgcc gagacgaagc tattcggtta atggagtacc
     5281 aagcgcaagg tgcggtgcat ttaatgctag gagtcgctgc ttctagagtt aagagcgata
     5341 accgctttag atactgccct gattgcgttg ctcttcagct aaataggtat ggggaagcct
     5401 tttggcaacg agattggtat ttgcccgctt tgccatattg tccaaaacac ggtgctttag
     5461 tcttctttga tagagctgta gatgatcacc gacatcaatt ttgggctttg ggtcatactg
     5521 agctgctttc agactacccc aaagactccc tatctcaatt aacagcacta gctgcttata
     5581 tagcccctct gttagatgct ccacgagcgc aagagctttc cccaagcctt gagcagtgga
     5641 cgctgtttta tcagcgctta gcgcaggatc tagggctaac caaaagcaag cacattcgtc
     5701 atgacttggt ggcggagaga gtgaggcaga cttttagtga tgaggcacta gagaaactgg
     5761 atttaaagtt ggcagagaac aaggacacgt gttggctgaa aagtatattc cgtaagcata
     5821 gaaaagcctt tagttattta cagcatagta ttgtgtggca agccttattg ccaaaactaa
     5881 cggttataga agcgctacag caggcaagtg ctcttactga gcactctata acgacaagac
     5941 ctgttagcca gtctgtgcaa cctaactctg aagatttatc tgttaagcat aaagactggc
     6001 agcaactagt gcataaatac caaggaatta aggcggcaag acagtcttta gagggtgggg
     6061 tgctatacgc ttggctttac cgacatgaca gggattggct agttcactgg aatcaacagc
     6121 atcaacaaga gcgtctggca cccgccccta gagttgattg gaaccaaaga gatcgaattg
     6181 ctgtacgaca actattaaga atcataaagc gtctagatag tagccttgat cacccaagag
     6241 cgacatcgag ctggctgtta aagcaaactc ctaacggaac ctctcttgca aaaaatctac
     6301 agaaactgcc tttggtagcg ctttgcttaa agcgttactc agagagtgtg gaagattatc
     6361 aaattagacg gattagccaa gcttttatta agcttaaaca ggaagatgtt gagcttaggc
     6421 gctggcgatt attaagaagt gcaacgttat ctaaagagcg gataactgag gaagcacaaa
     6481 gattcttgga aatggtttat ggggaagagt gagtggttag gctagctaca tttaatgaca
     6541 atgtgcaggt tgtacatatt ggtcatttat tccgtaactc gggtcataag gagtggcgta
     6601 tttttgtttg gtttaatcca atgcaagaac ggaaatggac tcgatttact catttgcctt
     6661 tattaagtcg agctaaggtg gttaacagta caacaaagca aataaataag gcggatcgtg
     6721 tgattgagtt tgaagcatcg gatcttcaac gagccaaaat aatcgatttt cctaatctct
     6781 cgtcctttgc ttccgtacgc aacaaggatg gagcgcagag ttcatttatt tacgaagctg
     6841 aaacaccata tagcaagact cgttatcaca tcccacagtt agagctagct cggtcattat
     6901 ttttagggga tcctctagag tcgacctgca ggcatgcaag cttggctgtt ttggcggatg
     6961 agagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca
     7021 gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt
     7081 gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca
     7141 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     7201 tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga
     7261 agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaatta
     7321 agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt ttgtttattt
     7381 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     7441 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     7501 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     7561 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     7621 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     7681 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     7741 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     7801 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     7861 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     7921 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     7981 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     8041 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     8101 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     8161 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     8221 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     8281 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     8341 tcatatatac tttagattga tttacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     8401 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     8461 tttcttccct tcctttctcg ccacgttcgc cgccggccag cctcgcagag caggattccc
     8521 gttgagcacc gccaggtgcg aataagggac agtgaagaag gaacacccgc tcgcgggtgg
     8581 gcctacttca cctatcctgc ccggcggcat caccggcgcc acaggtgcgg ttgctggcgc
     8641 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
     8701 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
     8761 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
     8821 cttcctaatg caggagtcgc ataagggaga gcgtcgatcc ccgacagtaa gacgggtaag
     8881 cctgttgatg ataccgctgc cttactgggt gcattagcca gtctgaatga cctgtcacgg
     8941 gataatccga agtggtcaga ctggaaaatc agagggcagg aactgctgaa cagcaaaaag
     9001 tcagatagca ccacatagca gacccgccat aaaacgccct gagaagcccg tgacgggctt
     9061 ttcttgtatt atgggtagtt tccttgcatg aatccataaa aggcgcctgt agtgccattt
     9121 acccccattc actgccagag ccgtgagcgc agcgaactga atgtcacgaa aaagacagcg
     9181 actcaggtgc ctgatggtcg gagacaaaag gaatattcag cgatttgccc gagcttgcga
     9241 gggtgctact taagccttta gggttttaag gtctgttttg tagaggagca aacagcgttt
     9301 gcgacatcct tttgtaatac tgcggaactg actaaagtag tgagttatac acagggctgg
     9361 gatctattct ttttatcttt ttttattctt tctttattct ataaattata accacttgaa
     9421 tataaacaaa aaaaacacac aaaggtctag cggaatttac agagggtcta gcagaattta
     9481 caagttttcc agcaaaggtc tagcagaatt tacagatacc cacaactcaa aggaaaagga
     9541 ctagtaatta tcattgacta gcccatctca attggtatag tgattaaaat cacctagacc
     9601 aattgagatg tatgtctgaa ttagttgttt tcaaagcaaa tgaactagcg attagtcgct
     9661 atgacttaac ggagcatgaa accaagctaa ttttatgctg tgtggcacta ctcaacccca
     9721 cgattgaaaa ccctacaagg aaagaacgga cggtatcgtt cacttataac caatacgttc
     9781 agatgatgaa catcagtagg gaaaatgctt atggtgtatt agctaaagca accagagagc
     9841 tgatgacgag aactgtggaa atcaggaatc ctttggttaa aggctttgag attttccagt
     9901 ggacaaacta tgccaagttc tcaagcgaaa aattagaatt agtttttagt gaagagatat
     9961 tgccttatct tttccagtta aaaaaattca taaaatataa tctggaacat gttaagtctt
    10021 ttgaaaacaa atactctatg aggatttatg agtggttatt aaaagaacta acacaaaaga
    10081 aaactcacaa ggcaaatata gagattagcc ttgatgaatt taagttcatg ttaatgcttg
    10141 aaaataacta ccatgagttt aaaaggctta accaatgggt tttgaaacca ataagtaaag
    10201 atttaaacac ttacagcaat atgaaattgg tggttgataa gcgaggccgc ccgactgata
    10261 cgttgatttt ccaagttgaa ctagatagac aaatggatct cgtaaccgaa cttgagaaca
    10321 accagataaa aatgaatggt gacaaaatac caacaaccat tacatcagat tcctacctac
    10381 ataacggact aagaaaaaca ctacacgatg ctttaactgc aaaaattcag ctcaccagtt
    10441 ttgaggcaaa atttttgagt gacatgcaaa gtaagtatga tctcaatggt tcgttctcat
    10501 ggctcacgca aaaacaacga accacactag agaacatact ggctaaatac ggaaggatct
    10561 gaggttctta tggctcttgt atctatcagt gaagcatcaa gactaacaaa caaaagtaga
    10621 acaactgttc accgttacat atcaaaggga aaactgtcca tgcacagatg aaaacggtgt
    10681 aaaaaagata gatacatcag agcttttacg agtttttggt gcatttaaag ctgttcacca
    10741 tgaacagatc gacaatgtaa cagatgaaca gcatgtaaca cctaatagaa caggtgaaac
    10801 cagtaaaaca aagcaactag aacatgaaat tgaacacctg agacaacttg ttacagctca
    10861 acagtcacac atagacagcc tgaaacaggc gatgctgctt atcgaatcaa agctgccgac
    10921 aacacgggag ccagtgacgc ctcccgtggg gaaaaaatca tggcaattct ggaagaaata
    10981 gcgctttcag ccggccacga tgcgtccggc gtagaggatc tgctcatgtt tgacagctta
    11041 tcatcgatgc ataatgtgcc tgtcaaatgg acgaagcagg gattctgcaa accctatgct
    11101 actccgtcaa gccgtcaatt gtctgattcg ttaccaatta tgacaacttg acggctacat
    11161 cattcacttt ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt
    11221 taaatacccg cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga
    11281 taggcatccg ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc
    11341 agcttaagac gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca
    11401 agcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga
    11461 tgtactgaca agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg
    11521 cttccatgcg ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc
    11581 gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt
    11641 gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat tgacggccag ttaagccatt
    11701 catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg
    11761 gatgacgacc gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc
    11821 aaacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa
    11881 tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa
    11941 tcggcgttaa acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat
    12001 tttgcgcttc agccatactt ttcatactcc cgccattcag ag
//
