LOCUS       sequence-308414-        9996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of N-term BirA tagged HDLBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9996)
  AUTHORS   Zinnall U, Milek M, Minia I, Vieira-Vieira CH, Muller S, Mastrobuoni
            G, Hazapis OG, Del Giudice S, Schwefel D, Bley N, Voigt F, Chao JA, 
            Kempa S, Huttelmaier S, Selbach M, Landthaler M
  TITLE     HDLBP binds ER-targeted mRNAs by multivalent interactions to promote
            protein synthesis of transmembrane and secreted proteins.
  JOURNAL   Nat Commun. 2022 May 18;13(1):2727. doi: 10.1038/s41467-022-30322-7.
  PUBMED    35585045
REFERENCE   2  (bases 1 to 9996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-05-18"; volume: "13(1):2727. doi"; pages: " 
            10.1038/s41467-022-30322-7"
COMMENT     Sequence Label: pcDNA5-BirA-FLAG-N-term-HDLBP
FEATURES             Location/Qualifiers
     source          1..9996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     16..36
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    67..85
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    88..106
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     116..140
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(262..612,613..615,616..1224)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             1255..1278
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1291..1315
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5129..5153)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5195..5212)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5201..5425
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5558..5577)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5709..5756
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5764..6786
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5764..6786
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6916..7037
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6953..6972)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7007..7026
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7086..7102)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7086..7102)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7099..7121)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7110..7126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7134..7140,7141..7158,7159..7164))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7179..7200
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7317..7334)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7488..8076)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7568..7587)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8247..9038,9039..9107))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8870..8889
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9108..9212)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9287..9306)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9478..9857
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(9858..9996,1..65)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
       61 gagctctccc tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc
      121 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
      181 gacaccggga ccgatccagc ctccggactc tagcgtttaa acttaagctt ggtaccgagc
      241 tcggatccag ctagcgccac catgaaggac aacaccgtgc ccctgaagct gatcgccctg
      301 ctggccaacg gcgagttcca cagcggcgag cagctgggcg agaccctggg catgagccgg
      361 gccgccatca acaagcacat ccagaccctg cgggactggg gcgtggacgt gttcaccgtg
      421 cccggcaagg gctacagcct gcccgagccc atccagctgc tgaacgccaa gcagatcctg
      481 ggccagctgg acggcggcag cgtggccgtg ctgcccgtga tcgacagcac caaccagtac
      541 ctgctggacc ggatcggcga gctgaagagc ggcgacgcct gcatcgccga gtaccagcag
      601 gccggccggg gcggccgggg ccggaagtgg ttcagcccct tcggcgccaa cctgtacctg
      661 agcatgttct ggcggctgga gcagggcccc gccgccgcca tcggcctgag cctggtgatc
      721 ggcatcgtga tggccgaggt gctgcggaag ctgggcgccg acaaggtgcg ggtgaagtgg
      781 cccaacgacc tgtacctgca ggaccggaag ctggccggca tcctggtgga gctgaccggc
      841 aagaccggcg acgccgccca gatcgtgatc ggcgccggca tcaacatggc catgcggcgg
      901 gtggaggaga gcgtggtgaa ccagggctgg atcaccctgc aggaggccgg catcaacctg
      961 gaccggaaca ccctggccgc catgctgatc cgggagctgc gggccgccct ggagctgttc
     1021 gagcaggagg gcctggcccc ctacctgagc cggtgggaga agctggacaa cttcatcaac
     1081 cggcccgtga agctgatcat cggcgacaag gagatcttcg gcatcagccg gggcatcgac
     1141 aagcagggcg ccctgctgct ggagcaggac ggcatcatca agccctggat gggcggcgag
     1201 atcagcctgc ggagcgccga gaagggggga tctggccccg gcggcggcgc gccagactac
     1261 aaagacgatg acgataaact aggcgcgccc acaagtttgt acaaaaaagc aggctccacc
     1321 atgagttccg ttgcagtttt gacccaagag agttttgctg aacaccgaag tgggctggtt
     1381 ccgcaacaaa tcaaagttgc cactctaaat tcagaagagg agagcgaccc tccaacctac
     1441 aaggatgcct tccctccact tcctgagaaa gctgcttgcc tggaaagtgc ccaggaaccc
     1501 gctggagcct gggggaacaa gatccgaccc atcaaggctt ctgtcatcac tcaggtgttc
     1561 catgtacccc tggaggagag aaaatacaag gatatgaacc agtttggaga aggtgaacaa
     1621 gcaaaaatct gccttgagat catgcagaga actggtgctc acttggagct gtctttggcc
     1681 aaagaccaag gcctctccat catggtgtca ggaaagctgg atgctgtcat gaaagctcgg
     1741 aaggacattg ttgctagact gcagactcag gcctcagcaa ctgttgccat tcccaaagaa
     1801 caccatcgct ttgttattgg caaaaatgga gagaaactgc aagacttgga gctaaaaact
     1861 gcaaccaaaa tccagatccc acgcccagat gaccccagca atcagatcaa gatcactggc
     1921 accaaagagg gcatcgagaa agctcgccat gaagtcttac tcatctctgc cgagcaggac
     1981 aaacgtgctg tggagaggct agaagtagaa aaggcattcc accccttcat cgctgggccg
     2041 tataatagac tggttggcga gatcatgcag gagacaggca cgcgcatcaa catcccccca
     2101 cccagcgtga accggacaga gattgtcttc actggagaga aggaacagtt ggctcaggct
     2161 gtggctcgca tcaagaagat ttatgaggag aagaaaaaga agactacaac cattgcagtg
     2221 gaagtgaaga aatcccaaca caagtatgtc attgggccca agggcaattc attgcaggag
     2281 atccttgaga gaactggagt ttccgttgag atcccaccct cagacagcat ctctgagact
     2341 gtaatacttc gaggcgaacc tgaaaagtta ggtcaggcgt tgactgaagt ctatgccaag
     2401 gccaatagct tcaccgtctc ctctgtcgcc gccccttcct ggcttcaccg tttcatcatt
     2461 ggcaagaaag ggcagaacct ggccaaaatc actcagcaga tgccaaaggt tcacatcgag
     2521 ttcacagagg gcgaagacaa gatcaccctg gagggcccta cagaggatgt caatgtggcc
     2581 caggaacaga tagaaggcat ggtcaaagat ttgattaacc ggatggacta tgtggagatc
     2641 aacatcgacc acaagttcca caggcacctc attgggaaga gcggtgccaa cataaacaga
     2701 atcaaagacc agtacaaggt gtccgtgcgc atccctcctg acagtgagaa gagcaatttg
     2761 atccgcatcg agggggaccc acagggcgtg cagcaggcca agcgagagct gctggagctt
     2821 gcatctcgca tggaaaatga gcgtaccaag gatctaatca ttgagcaaag atttcatcgc
     2881 acaatcattg ggcagaaggg tgaacggatc cgtgaaattc gtgacaaatt cccagaggtc
     2941 atcattaact ttccagaccc agcacaaaaa agtgacattg tccagctcag aggacctaag
     3001 aatgaggtgg aaaaatgcac aaaatacatg cagaagatgg tggcagatct ggtggaaaat
     3061 agctattcaa tttctgttcc gatcttcaaa cagtttcaca agaatatcat tgggaaagga
     3121 ggcgcaaaca ttaaaaagat tcgtgaagaa agcaacacca aaatcgacct tccagcagag
     3181 aatagcaatt cagagaccat tatcatcaca ggcaagcgag ccaactgcga agctgcccgg
     3241 agcaggattc tgtctattca gaaagacctg gccaacatag ccgaggtaga ggtctccatc
     3301 cctgccaagc tgcacaactc cctcattggc accaagggcc gtctgatccg ctccatcatg
     3361 gaggagtgcg gcggggtcca cattcacttt cccgtggaag gttcaggaag cgacaccgtt
     3421 gttatcaggg gcccttcctc ggatgtggag aaggccaaga agcagctcct gcatctggcg
     3481 gaggagaagc aaaccaagag tttcactgtt gacatccgcg ccaagccaga ataccacaaa
     3541 ttcctcatcg gcaagggggg cggcaaaatt cgcaaggtgc gcgacagcac tggagcacgt
     3601 gtcatcttcc ctgcggctga ggacaaggac caggacctga tcaccatcat tggaaaggag
     3661 gacgccgtcc gagaggcaca gaaggagctg gaggccttga tccaaaacct ggataatgtg
     3721 gtggaagact ccatgctggt ggaccccaag caccaccgcc acttcgtcat ccgcagaggc
     3781 caggtcttgc gggagattgc tgaagagtat ggcggggtga tggtcagctt cccacgctct
     3841 ggcacacaga gcgacaaagt caccctcaag ggcgccaagg actgtgtgga ggcagccaag
     3901 aaacgcattc aggagatcat tgaggacctg gaagctcagg tgacattaga atgtgctata
     3961 ccccagaaat tccatcgatc tgtcatgggc cccaaaggtt ccagaatcca gcagattact
     4021 cgggatttca gtgttcaaat taaattccca gacagagagg agaacgcagt tcacagtaca
     4081 gagccagttg tccaggagaa tggggacgaa gctggggagg ggagagaggc taaagattgt
     4141 gaccccggct ctccaaggag gtgtgacatc atcatcatct ctggccggaa agaaaagtgt
     4201 gaggctgcca aggaagctct ggaggcattg gttcctgtca ccattgaagt agaggtgccc
     4261 tttgaccttc accgttacgt tattgggcag aaaggaagtg ggatccgcaa gatgatggat
     4321 gagtttgagg tgaacataca tgtcccggca cctgagctgc agtctgacat catcgccatc
     4381 acgggcctcg ctgcaaattt ggaccgggcc aaggctggac tgctggagcg tgtgaaggag
     4441 ctacaggccg agcaggagga ccgggcttta aggagtttta agctgagtgt cactgtagac
     4501 cccaaatacc atcccaagat tatcgggaga aagggggcag taattaccca aatccggttg
     4561 gagcatgacg tgaacatcca gtttcctgat aaggacgatg ggaaccagcc ccaggaccaa
     4621 attaccatca cagggtacga aaagaacaca gaagctgcca gggatgctat actgagaatt
     4681 gtgggtgaac ttgagcagat ggtttctgag gacgtcccgc tggaccaccg cgttcacgcc
     4741 cgcatcattg gtgcccgcgg caaagccatt cgcaaaatca tggacgaatt caaggtggac
     4801 attcgcttcc cacagagcgg agccccagac cccaactgcg tcactgtgac ggggctccca
     4861 gagaatgtgg aggaagccat cgaccacatc ctcaatctgg aggaggaata cctagctgac
     4921 gtggtggaca gtgaggcgct gcaggtatac atgaaacccc cagcacacga agaggccaag
     4981 gcaccttcca gaggctttgt ggtgcgggac gcaccctgga ccgccagcag cagtgagaag
     5041 gctcctgaca tgagcagctc tgaggaattt cccagctttg gggctcaggt ggctcccaag
     5101 accctccctt ggggccccaa acgataggac ccagctttct tgtacaaagt ggtgtgactc
     5161 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     5221 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     5281 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     5341 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     5401 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     5461 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5521 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5581 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5641 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     5701 cgtacctaga agttcctatt ccgaagttcc tattctctag aaagtatagg aacttccttg
     5761 gccaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     5821 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     5881 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     5941 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     6001 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     6061 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     6121 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     6181 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     6241 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     6301 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     6361 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     6421 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     6481 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     6541 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     6601 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     6661 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     6721 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     6781 gaatagcacg tactacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     6841 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     6901 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6961 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7021 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     7081 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7141 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7201 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7261 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7321 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7381 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7441 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7501 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7561 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7621 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7681 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7741 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7801 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7861 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7921 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7981 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8041 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8101 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8161 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8221 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8281 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8341 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8401 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8461 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8521 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8581 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8641 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8701 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8761 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8821 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     8881 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8941 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9001 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9061 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9121 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9181 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     9241 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     9301 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     9361 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     9421 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
     9481 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     9541 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     9601 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     9661 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     9721 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     9781 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     9841 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     9901 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     9961 accaaaatca acgggacttt ccaaaatgtc gtaaca
//
