LOCUS       sequence-308422-       10116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing human BHLHE40.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10116)
  AUTHORS   Emming S, Bianchi N, Polletti S, Balestrieri C, Leoni C, Montagner 
            S, Chirichella M, Delaleu N, Natoli G, Monticelli S
  TITLE     A molecular network regulating the proinflammatory phenotype of 
            human memory T lymphocytes.
  JOURNAL   Nat Immunol. 2020 Apr;21(4):388-399. doi: 10.1038/s41590-020-0622-8.
            Epub 2020 Mar 16.
  PUBMED    32205878
REFERENCE   2  (bases 1 to 10116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41590-020-0622-8"; journalName: "Nat Immunol"; date: 
            "2020-04"; volume: "21"; issue: "4"; pages: "388-399"
COMMENT     Sequence Label: pLVX-EF1-alpha-BHLHE40-IRES-ZsGreen1
FEATURES             Location/Qualifiers
     source          1..10116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          231..1173
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1130..1150
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2465..3037
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2488..3039
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2632..2649)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             3039..3734
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     misc_feature    3748..4336
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3801..3821)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4219..4230)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4543..5176
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5304..5320)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5304..5320)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5317..5339)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5328..5344
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5352..5358,5359..5376,5377..5382))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5397..5418
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5535..5552)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5706..6294)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5786..5805)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6465..7256,7257..7325))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7088..7107
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7326..7430)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7478..7612
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7515..7534)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7569..7588
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7780..8413
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8460..8585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9082..9315
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9500..9544
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9807..9922
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        join(10116,1..1182)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
ORIGIN
        1 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
       61 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
      121 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
      181 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
      241 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
      301 cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
      361 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
      421 tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
      481 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
      541 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
      601 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
      661 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
      721 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
      781 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
      841 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
      901 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
      961 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     1021 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     1081 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     1141 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggatctat ttccggtgaa
     1201 ttcatggagc ggatccccag cgcgcaacca ccccccgcct gcctgcccaa agcaccggga
     1261 ctggagcacg gagacctacc agggatgtac cctgcccaca tgtaccaagt gtacaagtca
     1321 agacggggaa taaagcggag cgaggacagc aaggagacct acaaattgcc gcaccggctc
     1381 atcgagaaaa agagacgtga ccggattaac gagtgcatcg cccagctgaa ggatctccta
     1441 cccgaacatc tcaaacttac aactttgggt cacttggaaa aagcagtggt tcttgaactt
     1501 accttgaagc atgtgaaagc actaacaaac ctaattgatc agcagcagca gaaaatcatt
     1561 gccctgcaga gtggtttaca agctggtgag ctgtcaggga gaaatgtcga aacaggtcaa
     1621 gagatgttct gctcaggttt ccagacatgt gcccgggagg tgcttcagta tctggccaag
     1681 cacgagaaca ctcgggacct gaagtcttcg cagcttgtca cccacctcca ccgggtggtc
     1741 tcggagctgc tgcagggtgg tacctccagg aagccatcag acccagctcc caaagtgatg
     1801 gacttcaagg aaaaacccag ctctccggcc aaaggttcgg aaggtcctgg gaaaaactgc
     1861 gtgccagtca tccagcggac tttcgctcac tcgagtgggg agcagagcgg cagcgacacg
     1921 gacacagaca gtggctatgg aggagaatcg gagaagggcg acttgcgcag tgagcagccg
     1981 tgcttcaaaa gtgaccacgg acgcaggttc acgatgggag aaaggatcgg cgcaattaag
     2041 caagagtccg aagaaccccc cacaaaaaag aaccggatgc agctttcgga tgatgaaggc
     2101 catttcacta gcagtgacct gatcagctcc ccgttcctgg gcccacaccc acaccagcct
     2161 cctttctgcc tgcccttcta cctgatccca ccttcagcga ctgcctacct gcccatgctg
     2221 gagaagtgct ggtatcccac ctcagtgcca gtgctatacc caggcctcaa cgcctctgcc
     2281 gcagccctct ctagcttcat gaacccagac aagatctcgg ctcccttgct catgccccag
     2341 agactccctt ctcccttgcc agctcatccg tccgtcgact cttctgtctt gctccaagct
     2401 ctgaagccaa tccccccttt aaacttagaa accaaagact aatctagagc ggccgcggat
     2461 cccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     2521 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     2581 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     2641 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     2701 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     2761 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     2821 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctcacctca agcgtattca
     2881 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     2941 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca
     3001 cggggacgtg gttttccttt gaaaaacacg atgataatat ggcccagtcc aagcacggcc
     3061 tgaccaagga gatgaccatg aagtaccgca tggagggctg cgtggacggc cacaagttcg
     3121 tgatcaccgg cgagggcatc ggctacccct tcaagggcaa gcaggccatc aacctgtgcg
     3181 tggtggaggg cggccccttg cccttcgccg aggacatctt gtccgccgcc ttcatgtacg
     3241 gcaaccgcgt gttcaccgag tacccccagg acatcgtcga ctacttcaag aactcctgcc
     3301 ccgccggcta cacctgggac cgctccttcc tgttcgagga cggcgccgtg tgcatctgca
     3361 acgccgacat caccgtgagc gtggaggaga actgcatgta ccacgagtcc aagttctacg
     3421 gcgtgaactt ccccgccgac ggccccgtga tgaagaagat gaccgacaac tgggagccct
     3481 cctgcgagaa gatcatcccc gtgcccaagc agggcatctt gaagggcgac gtgagcatgt
     3541 acctgctgct gaaggacggt ggccgcttgc gctgccagtt cgacaccgtg tacaaggcca
     3601 agtccgtgcc ccgcaagatg cccgactggc acttcatcca gcacaagctg acccgcgagg
     3661 accgcagcga cgccaagaac cagaagtggc acctgaccga gcacgccatc gcctccggct
     3721 ccgccttgcc ctgaacgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga
     3781 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     3841 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     3901 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     3961 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     4021 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     4081 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     4141 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     4201 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     4261 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     4321 tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa
     4381 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     4441 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     4501 cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact
     4561 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     4621 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
     4681 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
     4741 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
     4801 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
     4861 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
     4921 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     4981 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     5041 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     5101 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     5161 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     5221 aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgtttacc gtcgacctct
     5281 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     5341 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     5401 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     5461 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     5521 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     5581 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     5641 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     5701 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     5761 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     5821 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     5881 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     5941 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6001 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6061 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6121 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     6181 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6241 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6301 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6361 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     6421 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     6481 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     6541 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     6601 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     6661 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     6721 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     6781 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     6841 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     6901 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     6961 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     7021 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7081 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7141 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7201 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7261 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7321 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7381 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     7441 cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa
     7501 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     7561 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
     7621 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
     7681 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
     7741 aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc
     7801 aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     7861 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     7921 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     7981 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     8041 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     8101 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     8161 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     8221 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8281 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8341 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8401 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     8461 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     8521 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     8581 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     8641 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     8701 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     8761 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     8821 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     8881 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt
     8941 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     9001 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     9061 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     9121 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     9181 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     9241 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     9301 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     9361 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     9421 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     9481 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     9541 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     9601 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     9661 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     9721 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     9781 agtgaacgga tctcgacggt atcgccttta aaagaaaagg ggggattggg gggtacagtg
     9841 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     9901 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttatc
     9961 gatgagtaat tcatacaaaa ggactcgccc ctgccttggg gaatcccagg gaccgtcgtt
    10021 aaactcccac taacgtagaa cccagagatc gctgcgttcc cgccccctca cccgcccgct
    10081 ctcgtcatca ctgaggtgga gaagagcatg cgtgag
//
