LOCUS       sequence-308425-       11316 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one inducible LV expression construct for Ngn2:2A:Ascl1 (aka 
            UNA; for direct neuronal conversion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11316)
  AUTHORS   Herdy J, Schafer S, Kim Y, Ansari Z, Zangwill D, Ku M, Paquola A, 
            Lee H, Mertens J, Gage FH
  TITLE     Chemical modulation of transcriptionally enriched signaling pathways
            to optimize the conversion of fibroblasts into neurons.
  JOURNAL   Elife. 2019 May 17;8. pii: 41356. doi: 10.7554/eLife.41356.
  PUBMED    31099332
REFERENCE   2  (bases 1 to 11316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-05-17"; volume: "8. pii: 41356. doi"; pages: " 
            10.7554/eLife.41356"
COMMENT     Sequence Label: pLVX-UbC-rtTA-Ngn2:2A:Ascl1
FEATURES             Location/Qualifiers
     source          1..11316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    2..20
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    38..56
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    73..91
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    109..127
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    145..163
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    180..198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    216..234
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     281..305
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1161..1217
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        1951..2450
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1986..2009)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2383..2402
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2471..3070
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2471..2490)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2967..2987
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3087..3675
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3140..3160)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3558..3569)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3882..4515
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4643..4659)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4643..4659)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4656..4678)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4667..4683
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4691..4697,4698..4715,4716..4721))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4736..4757
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4874..4891)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5045..5633)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5125..5144)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5804..6595,6596..6664))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6427..6446
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6665..6769)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6817..6951
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6854..6873)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6908..6927
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7119..7752
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7799..7924
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8421..8654
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8839..8883
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9146..9261
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(join(9302..9313,9314..9349,9350..9352,9353..9388,
                     9389..9391,9392..9427,9428..9433,9434..10051))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPGN"
     promoter        complement(10061..11272)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(10205..10224)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     promoter        join(11310..11316,1..308)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
ORIGIN
        1 ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacga
       61 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
      121 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttat
      181 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat
      241 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
      301 agatcgcctg gagaaggatc cgcggccgcg ccgccaccat gttcgtcaaa tccgagacct
      361 tggagttgaa ggaggaagag gacgtgttag tgctgctcgg atcggcctcc cccgccttgg
      421 cggccctgac cccgctgtca tccagcgccg acgaagaaga ggaggaggag ccgggcgcgt
      481 caggcggggc gcgtcggcag cgcggggctg aggccgggca gggggcgcgg ggcggcgtgg
      541 ctgcgggtgc ggagggctgc cggcccgcac ggctgctggg tctggtacac gattgcaaac
      601 ggcgcccttc ccgggcgcgg gccgtctccc gaggcgccaa gacggccgag acggtgcagc
      661 gcatcaagaa gacccgtaga ctgaaggcca acaaccgcga gcgaaaccgc atgcacaacc
      721 tcaacgcggc actggacgcg ctgcgcgagg tgctccccac gttccccgag gacgccaagc
      781 tcaccaagat cgagaccctg cgcttcgccc acaactacat ctgggcactc accgagaccc
      841 tgcgcctggc ggatcactgc gggggcggcg gcgggggcct gccgggggcg ctcttctccg
      901 aggcagtgtt gctgagcccg ggaggcgcca gcgccgccct gagcagcagc ggagacagcc
      961 cctcgcccgc ctccacgtgg agttgcacca acagccccgc gccgtcctcc tccgtgtcct
     1021 ccaattccac ctccccctac agctgcactt tatcgcccgc cagcccggcc gggtcagaca
     1081 tggactattg gcagccccca cctcccgaca agcaccgcta tgcacctcac ctccccatag
     1141 ccagggattg tatccatatg gctaccaact tctcacttct caagcaggct ggtgacgtcg
     1201 aagagaatcc tggcccaatg gaaagctctg ccaagatgga gagcggcggc gccggccagc
     1261 agccccagcc gcagccccag cagcccttcc tgccgcccgc agcctgtttc tttgccacgg
     1321 ccgcagccgc ggcggccgca gccgccgcag cggcagcgca gagcgcgcag cagcagcagc
     1381 agcagcagca gcagcagcag cagcaggcgc cgcagctgag accggcggcc gacggccagc
     1441 cctcaggggg cggtcacaag tcagcgccca agcaagtcaa gcgacagcgc tcgtcttcgc
     1501 ccgaactgat gcgctgcaaa cgccggctca acttcagcgg ctttggctac agcctgccgc
     1561 agcagcagcc ggccgccgtg gcgcgccgca acgagcgcga gcgcaaccgc gtcaagttgg
     1621 tcaacctggg ctttgccacc cttcgggagc acgtccccaa cggcgcggcc aacaagaaga
     1681 tgagtaaggt ggagacactg cgctcggcgg tcgagtacat ccgcgcgctg cagcagctgc
     1741 tggacgagca tgacgcggtg agcgccgcct tccaggcagg cgtcctgtcg cccaccatct
     1801 cccccaacta ctccaacgac ttgaactcca tggccggctc gccggtctca tcctactcgt
     1861 cggacgaggg ctcttacgac ccgctcagcc ccgaggagca ggagcttctc gacttcacca
     1921 actggttctg atagacgcgt gaattctacc gggtagggga ggcgcttttc ccaaggcagt
     1981 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     2041 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     2101 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     2161 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     2221 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     2281 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
     2341 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
     2401 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
     2461 caagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     2521 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     2581 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     2641 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     2701 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     2761 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     2821 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     2881 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     2941 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     3001 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     3061 cggtgcctga cgggcgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat
     3121 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     3181 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     3241 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     3301 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     3361 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     3421 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     3481 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     3541 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     3601 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     3661 gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat
     3721 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     3781 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     3841 agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc
     3901 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     3961 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     4021 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
     4081 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
     4141 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
     4201 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
     4261 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
     4321 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     4381 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     4441 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     4501 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     4561 agaaatgaat atcagagagt gagaggcctt gacattgcta gcgtttaccg tcgacctcta
     4621 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     4681 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     4741 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     4801 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     4861 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     4921 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     4981 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     5041 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5101 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5161 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5221 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     5281 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5341 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5401 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5461 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     5521 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5581 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     5641 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     5701 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     5761 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     5821 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     5881 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     5941 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     6001 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     6061 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     6121 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     6181 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     6241 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     6301 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     6361 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     6421 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     6481 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     6541 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     6601 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     6661 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     6721 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     6781 gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc agcttataat
     6841 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6901 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
     6961 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
     7021 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
     7081 aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca
     7141 aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta
     7201 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa
     7261 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt
     7321 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag
     7381 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa
     7441 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg
     7501 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt
     7561 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7621 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7681 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7741 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     7801 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     7861 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     7921 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     7981 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     8041 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     8101 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     8161 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     8221 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc
     8281 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     8341 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     8401 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     8461 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     8521 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     8581 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     8641 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     8701 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     8761 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     8821 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     8881 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     8941 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     9001 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     9061 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     9121 gtgaacggat ctcgacggta tcgcctttaa aagaaaaggg gggattgggg ggtacagtgc
     9181 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     9241 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttatcg
     9301 attagttacc cgggagcatg tcaaggtcaa aatcgtcaag agcgtcagca ggcagcatat
     9361 caaggtcaaa gtcgtcaagg gcatcggctg ggagcatgtc taagtcaaaa tcgtcaaggg
     9421 cgtcggccgg cccgccgctt tcgcacttta gctgtttctc caggccacat atgattagtt
     9481 ccaggccgaa aaggaaggca ggttcggctc cctgccggtc gaacagctca attgcttgtc
     9541 tcagaagtgg gggcatagaa tcggtggtag gtgtctctct ttcctctttt gctacttgat
     9601 gctcctgttc ctccaatacg cagcccagtg taaagtggcc cacggcggac agagcgtaca
     9661 gtgcgttctc cagggagaag ccttgctgac acaggaacgc gagctgattt tccagggttt
     9721 cgtactgttt ctctgttggg cgggtgccga gatgcacttt agccccgtcg cgatgtgaga
     9781 ggagagcaca gcggaatgac ttggcgttgt tccgcagaaa gtcttgccat gactcgcctt
     9841 ccagggggca gaagtgggta tgatgcctgt ccagcatctc gattggcagg gcatcgagca
     9901 gggcccgctt gttcttcacg tgccagtaca gggtaggctg ctcaactccc agcttttgag
     9961 cgagtttcct tgtcgtcagg ccttcgatac cgactccatt gagtaattcc agagcgccgt
    10021 ttatgacttt gctcttgtcc agtctagaca tggtggatcc gtctaacaaa aaagccaaaa
    10081 acggccagaa tttagcggac aatttactag tctaacactg aaaattacat attgacccaa
    10141 atgattacat ttcaaaaggt gcctaaaaaa cttcacaaaa cacactcgcc aaccccgagc
    10201 gcatagttca aaaccggagc ttcagctact taagaagata ggtacataaa accgaccaaa
    10261 gaaactgacg cctcacttat ccctcccctc accagaggtc cggcgcctgt cgattcagga
    10321 gagcctaccc taggcccgaa ccctgcgtcc tgcgacggag aaaagcctac cgcacaccta
    10381 ccggcaggtg gccccaccct gcattataag ccaacagaac gggtgacgtc acgacacgac
    10441 gagggcgcgc gctcccaaag gtacgggtgc actgcccaac ggcaccgcca taactgccgc
    10501 ccccgcaaca gacgacaaac cgagttctcc agtcagtgac aaacttcacg tcagggtccc
    10561 cagatggtgc cccagcccat ctcacccgaa taagagcttt cccgcattag cgaaggcctc
    10621 aagaccttgg gttcttgccg cccaccatgc cccccacctt gtttcaacga cctcacagcc
    10681 cgcctcacaa gcgtcttcca ttcaagactc gggaacagcc gccattttgc tgcgctcccc
    10741 ccaaccccca gttcagggca accttgctcg cggacccaga ctacagccct tggcggtctc
    10801 tccacacgct tccgtcccac cgagcggccc ggcggccacg aaagccccgg ccagcccagc
    10861 agcccgctac tcaccaagtg acgatcacag cgatccacaa acaagaaccg cgacccaaat
    10921 cccggctgcg acggaactag ctgtgccaca cccggcgcgt ccttatataa tcatcggcgt
    10981 tcaccgcccc acggagatcc ctccgcagaa tcgccgagaa gggactactt ttcctcgcct
    11041 gttccgctct ctggaaagaa aaccagtgcc ctagagtcac ccaagtcccg tcctaaaatg
    11101 tccttctgct gatactgggg ttctaaggcc gagtcttatg agcagcgggc cgctgtcctg
    11161 agcgtccggg cggaaggatc aggacgctcg ctgcgccctt cgtctgacgt ggcagcgctc
    11221 gccgtgagga ggggggcgcc cgcgggaggc gccaaaaccc ggcgcggagg ccagatcggc
    11281 gcatcgatga ggccctttcg tcttcactcg agttta
//
