LOCUS       sequence-308436-        9368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral backbone for cloning and expressing U6 driven hybrid 
            guide (hg)RNAs with BfuAI cloning sites and puromycin selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9368)
  AUTHORS   Gonatopoulos-Pournatzis T, Aregger M, Brown KR, Farhangmehr S, 
            Braunschweig U, Ward HN, Ha KCH, Weiss A, Billmann M, Durbic T, 
            Myers CL, Blencowe BJ, Moffat J
  TITLE     Genetic interaction mapping and exon-resolution functional genomics 
            with a hybrid Cas9-Cas12a platform.
  JOURNAL   Nat Biotechnol. 2020 May;38(5):638-648. doi: 
            10.1038/s41587-020-0437-z. Epub 2020 Mar 16.
  PUBMED    32249828
REFERENCE   2  (bases 1 to 9368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0437-z"; journalName: "Nat Biotechnol"; date: 
            "2020-05"; volume: "38"; issue: "5"; pages: "638-648"
COMMENT     Sequence Label: pLCHKO
FEATURES             Location/Qualifiers
     source          1..9368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        complement(3618..3866)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(3676..3695)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(3846..3866)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    3908..4025
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4074..4584
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4503..4521
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4606..5205
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4606..4625)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5102..5122
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5221..5809
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5274..5294)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5692..5703)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5881..6114
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6192..6313
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6229..6248)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6283..6302
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6353..6488
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6508..6527)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6509..6527)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6537..6554)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6537..6553)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6546..6568)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6695..7150
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6782..6801)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6992..7013
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7176..7280
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7281..7349,7350..8141)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7499..7518)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8312..8900
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8801..8820
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9054..9071
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9188..9209
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9224..9229,9230..9247,9248..9254)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9262..9278
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9267..9289
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9286..9302
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9286..9302
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9321..9341
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9323..9341
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccca gagaattcaa aaaaaactct gcaggtctac
     1741 aaaaaagagc aagaagctaa aaaagattta aaaattattt ttagcgcagt taatggaaca
     1801 ggaactaaat ttaccccaaa aatattacgt gaatcaggat ataacgttat tgaggttgaa
     1861 gagcatgcat ttgaagatga aacatttaaa aatgttgtaa atccaaatcc agaatttgat
     1921 cctgcatgaa aaataccgct tgaatatggt attaaacatg atgcagatat tattattatg
     1981 aatgacccag atgctgacag atttggaatg gcaataaaac atgatggtca ttttgtaaga
     2041 ttagatggaa atcaaacagg accaatttta attgattgaa aattatcaaa tctaaaacgc
     2101 ttaaatagca ttccaaaaaa tccggctcta tattcaagtt ttgtaacaag tgatttgggt
     2161 gatagaatcg ctcatgaaaa atatggagtt aatattgtaa aaactttaac tggatttaaa
     2221 tgaatgggta gagaaattgc taaagaagaa gataacggat taaattttgt ttttgcttat
     2281 gaagaaagtt atggatatgt aattgatgac tcagctagag ataaagatgg aatacaagct
     2341 tctatattaa tagcagaggc tgcttgattt tataaaaaac aaaataaaac attagtagac
     2401 tatttagaag atttatttaa agaaatgggt gcatattaca ctttcacttt aaacttgaat
     2461 tttaaaccag aagaaaagaa attaaaaatt gaaccattaa tgaaatcatt gagagcaaca
     2521 cccttaactc aaattgctgg acttaaagtt gttaatgttg aagactacat cgatggaatg
     2581 tataatatgc caggacaaga cttactaaaa ttttatttag aagataagtc atgatttgct
     2641 gttcgcccaa gtggaactga acctaaacta aaaatttatt ttataggtgt tggtgaatct
     2701 gttcaaaacg ctaaagttaa agtagacgaa attattaaag aattaaaatt aaaaatgaat
     2761 atataggaga aaaaatgaaa ctaaacaaat atatagatca cacattatta aaacaagatg
     2821 ctacgaaagc tgaaattaaa caattatgtg atgaagcaat tgaatttgat tttgcaacag
     2881 tttgtgttaa ttcatattga acaagctatt gtaaagaatt attaaaaggc acaaatgtag
     2941 gaataacaaa tgttgtaggt tttcctctag gtgcatgcac aacagctaca aaagcattcg
     3001 aagtttctga agcaattaaa gatggtgcaa cagaaattga tatggtatta aatattggtg
     3061 cattaaaaga caaaaattat gaattagttt tagaagacat gaaagctgta aaaaaagcag
     3121 ctggatcaca tgttgttaaa tgtattatgg aaaattgttt attaacaaaa gaagaaatca
     3181 tgaaagcttg tgaaatagct gttgaagctg gattagaatt tgttaaaaca tcaacaggat
     3241 tttcaaaatc aggtgcaaca tttgaagatg ttaaactaat gaagtcagtt gttaaagaca
     3301 atgctttagt taaagcagct ggtggagtta gaacatttga agatgctcaa aaaatgattg
     3361 aagcaggagc tgaccgctta ggaacaagtg gtggagtagc tattattaaa ggtgaagaaa
     3421 acaacgcgag ttactaaaac tagcgttttt ttattttgct catttttatt aaaagtttgc
     3481 aaaaaggaac ataaaaattc taattattga tactaaagtt attaaaaaga agattttggt
     3541 tgattttata aaggtcatag aatataatat tttagcatgt gtattttgtg tgctcattta
     3601 caacacacct gcctaccggt acctcgtcct ttccacaaga tatataaagc caagaaatcg
     3661 aaatactttc aagttacggt aagcatatga tagtccattt taaaacataa ttttaaaact
     3721 gcaaactacc caagaaatta ttactttcta cgtcacgtat tttgtactaa tatctttgtg
     3781 tttacagtca aattaattct aattatctct ctaacagcct tgtatcgtat atgcaaatat
     3841 gaaggaatca tgggaaatag gccctcgaat tctagatctt gagacaaatg gcagtattca
     3901 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     3961 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     4021 attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc gagggggccc
     4081 gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt
     4141 ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc
     4201 agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg
     4261 cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc
     4321 gcagctcaca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga
     4381 ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg
     4441 gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg
     4501 cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac
     4561 cgaatcaccg acctctctcc ccagggggat ccaccggagc ttaccatgac cgagtacaag
     4621 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     4681 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     4741 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     4801 gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     4861 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     4921 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     4981 gccaccgtcg gtgtttcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     5041 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     5101 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     5161 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaacgcg ttaagtcgac
     5221 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     5281 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     5341 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     5401 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     5461 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     5521 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     5581 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     5641 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     5701 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     5761 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     5821 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     5881 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     5941 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     6001 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6061 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
     6121 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     6181 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     6241 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     6301 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     6361 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6421 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     6481 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     6541 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     6601 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     6661 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     6721 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6781 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6841 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6901 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6961 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     7021 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     7081 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     7141 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7201 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7261 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     7321 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     7381 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     7441 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     7501 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     7561 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     7621 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     7681 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7741 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7801 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     7861 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7921 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7981 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     8041 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     8101 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     8161 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     8221 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8281 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     8341 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     8401 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     8461 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     8521 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     8581 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8641 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8701 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8761 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8821 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8881 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8941 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     9001 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     9061 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     9121 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     9181 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     9241 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     9301 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     9361 aagcttaa
//
