LOCUS       sequence-308557-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express N-ter FLAG HA tagged DHX36 isoform 2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Sauer M, Juranek SA, Marks J, De Magis A, Kazemier HG, Hilbig D, 
            Benhalevy D, Wang X, Hafner M, Paeschke K
  TITLE     DHX36 prevents the accumulation of translationally inactive mRNAs 
            with G4-structures in untranslated regions.
  JOURNAL   Nat Commun. 2019 Jun 3;10(1):2421. doi: 10.1038/s41467-019-10432-5.
  PUBMED    31160600
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-06-3"; volume: "10(1):2421. doi"; pages: " 
            10.1038/s41467-019-10432-5"
COMMENT     Sequence Label: pFRT-DHX36iso2
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    3..21
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    24..42
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     52..76
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             167..190
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             191..217
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tctccctatc agtgatagag atctccctat cagtgataga gatcgtcgac gagctcgttt
       61 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
      121 ccgggaccga tccagcctcc ggactctagc gtttaaactt aagatggact acaaggacga
      181 cgatgacaag tacccatacg atgttccaga ttacgctgga tccatgagtt atgactacca
      241 tcagaactgg ggccgtgatg ggggtccccg cagctccggt gggggctatg gaggggggcc
      301 agcagggggt catggaggta accgaggctc cggaggaggc ggcggcggcg gagggggtgg
      361 tcgaggcggc aggggccggc atcccgggca cctgaaaggc cgcgaaatcg gcatgtggta
      421 cgcgaaaaaa caggggcaga agaacaagga agcggagagg caagagagag ctgtagtaca
      481 catggatgaa cgacgagaag aacaaattgt acagttactg aattctgttc aagcgaagaa
      541 tgataaagag tcagaagcac agatatcctg gtttgctcct gaggatcatg gatacggtac
      601 tgaagtttct actaagaaca caccatgctc agagaacaaa cttgacatcc aggaaaagaa
      661 gttgataaat caagaaaaaa aaatgtttag aatcaggaac agatcatata ttgaccgaga
      721 ttctgagtat ctcttgcaag aaaatgaacc agatggaact ttagaccaaa aattattgga
      781 agatttacaa aagaaaaaaa atgaccttcg gtatattgaa atgcagcatt tcagagaaaa
      841 gctgccttcg tatggaatgc aaaaggaatt ggtaaattta attgataacc atcaggtaac
      901 agtaataagt ggtgaaactg gttgtggcaa accactcaag ttactcagtt cattttgga
//
