LOCUS       sequence-30857-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Wang L, Jin Q, Lee JE, Su IH, Ge K
  TITLE     Histone H3K27 methyltransferase Ezh2 represses Wnt genes to 
            facilitate adipogenesis.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Apr 20. 107(16):7317-22.
  PUBMED    20368440
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Apr 20. 107(16):7317-22."
COMMENT     Sequence Label: MSCVhygro-F-Ezh2-F667I
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..69
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cctcactcct tctctaggcg ccggaattag atcctggcca ccatggacta caaggacgac
       61 gatgacaaga tgggccagac tgggaagaaa tctgagaagg gaccggtttg ttggcggaag
      121 cgtgtaaaat cagagtacat gagactgaga cagctcaaga ggttcagaag agctgatgaa
      181 gtaaagacta tgtttagttc caatcgtcag aaaattttgg aaagaactga aaccttaaac
      241 caagagtgga agcagcggag gatacagcct gtgcacatca tgacttctgt gagctcattg
      301 cgcgggacta gggagtgttc agtcaccagt gacttggatt ttccagcaca agtcatcccg
      361 ttaaagaccc tgaatgcagt cgcctcggtg cctataatgt actcttggtc gcccttacaa
      421 cagaatttta tggtggaaga cgaaactgtc ttacataaca ttccttacat gggggatgaa
      481 gttctggatc aggacggcac tttcattgaa gaactaataa aaaattatga tggaaaagtg
      541 catggtgaca gagaatgtgg atttataaat gatgaaattt ttgtggagtt ggtaaatgct
      601 cttggtcaat ataatgatga tgatgatgac gatgatggag atgatccaga tgaaagagaa
      661 gaaaaacaga aagatctaga ggataatcga gatgataaag aaacttgccc acctcggaaa
      721 tttcctgctg ataaaatatt tgaagccatt tcctcaatgt ttccagataa gggcaccgca
      781 gaagaactga aggaaaaata taaagaactc acggagcagc agctcccnnt gctctgcctc
      841 ctgaatgtac tccaaacatc gatggaccaa atgccaaatc tgttca
//
