LOCUS       sequence-308591-       11057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  parental, all-in-one CRISPRi vector for B. burgdorferi, parental for
            gRNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11057)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 11057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9G(RBSmut)_arr2
FEATURES             Location/Qualifiers
     source          1..11057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..15,16..21,22..38,39..45)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    21..37
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    53..69
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             join(106..132,133..135,136..2622,2623..2625,2626..4212)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4382..4915
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     rep_origin      5038..5626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5527..5546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcataaaaaa tttatttgct ttgtgagcgg ataacaatta taatagattc aattgtgagc
       61 ggataacaat ttcacacaga attcattaaa gaagagaaat tacatatgga taagaaatac
      121 tcaataggct tagctatcgg cacaaatagc gtcggatggg cggtgatcac tgatgaatat
      181 aaggttccgt ctaaaaagtt caaggttctg ggaaatacag accgccacag tatcaaaaaa
      241 aatcttatag gggctctttt atttgacagt ggagagacag cggaagcgac tcgtctcaaa
      301 cggacagctc gtagaaggta tacacgtcgg aagaatcgta tttgttatct acaggagatt
      361 ttttcaaatg agatggcgaa agtagatgat agtttctttc atcgacttga agagtctttt
      421 ttggtggaag aagacaagaa gcatgaacgt catcctattt ttggaaatat agtagatgaa
      481 gttgcttatc atgagaaata tccaactatc tatcatctgc gaaaaaaatt ggtagattct
      541 actgataaag cggatttgcg cttaatctat ttggccttag cgcatatgat taagtttcgt
      601 ggtcattttt tgattgaggg agatttaaat cctgataata gtgatgtgga caaactattt
      661 atccagttgg tacaaaccta caatcaatta tttgaagaaa accctattaa cgcaagtgga
      721 gtagatgcta aagcgattct ttctgcacga ttgagtaaat caagacgatt agaaaatctc
      781 attgctcagc tccccggtga gaagaaaaat ggcttatttg ggaatctcat tgctttgtca
      841 ttgggtttga cccctaattt taaatcaaat tttgatttgg cagaagatgc taaattacag
      901 ctttcaaaag atacttacga tgatgattta gataatttat tggcgcaaat tggagatcaa
      961 tatgctgatt tgtttttggc agctaagaat ttatcagatg ctattttact ttcagatatc
     1021 ctaagagtaa atactgaaat aactaaggct cccctatcag cttcaatgat taaacgctac
     1081 gatgaacatc atcaagactt gactctttta aaagctttag ttcgacaaca acttccagaa
     1141 aagtataaag aaatcttttt tgatcaatca aaaaacggat atgcaggtta tattgatggg
     1201 ggagctagcc aagaagaatt ttataaattt atcaaaccaa ttttagaaaa aatggatggt
     1261 actgaggaat tattggtgaa actaaatcgt gaagatttgc tgcgcaagca acggaccttt
     1321 gacaacggct ctattcccca tcaaattcac ttgggtgagc tgcatgctat tttgagaaga
     1381 caagaagact tttatccatt tttaaaagac aatcgtgaga agattgaaaa aatcttgact
     1441 tttcgaattc cttattatgt tggtccattg gcgcgtggca atagtcgttt tgcatggatg
     1501 actcggaagt ctgaagaaac aattacccca tggaattttg aagaagttgt cgataaaggt
     1561 gcttcagctc aatcatttat tgaacgcatg acaaactttg ataaaaatct tccaaatgaa
     1621 aaagtactac caaaacatag tttgctttat gagtatttta cggtttataa cgaattgaca
     1681 aaggtcaaat atgttactga aggaatgcga aaaccagcat ttctttcagg tgaacagaag
     1741 aaagccattg ttgatttact cttcaaaaca aatcgaaaag taaccgttaa gcaattaaaa
     1801 gaagattatt tcaaaaaaat agaatgtttt gatagtgttg aaatttcagg agttgaagat
     1861 agatttaatg cttcattagg tacctaccat gatttgctaa aaattattaa agataaagat
     1921 tttttggata atgaagaaaa tgaagatatc ttagaggata ttgttttaac attgacctta
     1981 tttgaagata gggagatgat tgaggaaaga cttaaaacat atgctcacct ctttgatgat
     2041 aaggtgatga aacagcttaa acgtcgccgt tatactggtt ggggacgttt gtctcgaaaa
     2101 ttgattaatg gtattaggga taagcaatct ggcaaaacaa tattagattt tttgaaatca
     2161 gatggttttg ccaatcgcaa ttttatgcag ctgatccatg atgatagttt gacatttaaa
     2221 gaagacattc aaaaagcaca agtgtctgga caaggcgata gtttacatga acatattgca
     2281 aatttagctg gtagccctgc tattaaaaaa ggtattttac agactgtaaa agttgttgat
     2341 gaattggtca aagtaatggg gcggcataag ccagaaaata tcgttattga aatggcacgt
     2401 gaaaatcaga caactcaaaa gggccagaaa aattcgcgag agcgtatgaa acgaatcgaa
     2461 gaaggtatca aagaattagg aagtcagatt cttaaagagc atcctgttga aaatactcaa
     2521 ttgcaaaatg aaaagctcta tctctattat ctccaaaatg gaagagacat gtatgtggac
     2581 caagaattag atattaatcg tttaagtgat tatgatgtcg atgccattgt tccacaaagt
     2641 ttccttaaag acgattcaat agacaataag gtcttaacgc gttctgataa aaatcgtggt
     2701 aaatcggata acgttccaag tgaagaagta gtcaaaaaga tgaaaaacta ttggagacaa
     2761 cttctaaacg ccaagttaat cactcaacgt aagtttgata atttaacgaa agctgaacgt
     2821 ggaggtttga gtgaacttga taaagctggt tttatcaaac gccaattggt tgaaactcgc
     2881 caaatcacta agcatgtggc acaaattttg gatagtcgca tgaatactaa atacgatgaa
     2941 aatgataaac ttattcgaga ggttaaagtg attaccttaa aatctaaatt agtttctgac
     3001 ttccgaaaag atttccaatt ctataaagta cgtgagatta acaattacca tcatgcccat
     3061 gatgcgtatc taaatgccgt cgttggaact gctttgatta agaaatatcc aaaacttgaa
     3121 tcggagtttg tctatggtga ttataaagtt tatgatgttc gtaaaatgat tgctaagtct
     3181 gagcaagaaa taggcaaagc aaccgcaaaa tatttctttt actctaatat catgaacttc
     3241 ttcaaaacag aaattacact tgcaaatgga gagattcgca aacgccctct aatcgaaact
     3301 aatggggaaa ctggagaaat tgtctgggat aaagggcgag attttgccac agtgcgcaaa
     3361 gtattgtcca tgccccaagt caatattgtc aagaaaacag aagtccagac aggcggattc
     3421 tccaaggagt caattttacc aaaaagaaat tcggacaagc ttattgctcg taaaaaagac
     3481 tgggatccaa aaaaatatgg tggttttgat agtccaacgg tagcttattc agtcctagtg
     3541 gttgctaagg tggaaaaagg gaaatcgaag aagttaaaat ccgttaaaga gttactaggg
     3601 atcacaattc tggaaagaag ttcctttgaa aaaaatccga ttgacttttt agaagctaaa
     3661 ggatataagg aagttaaaaa agacttaatc attaaactac ctaaatatag tctttttgag
     3721 ttagaaaacg gtcgtaaacg gatgctggct agtgccggag aattacaaaa aggaaatgag
     3781 ctggctctgc caagcaaata tgtgaatttt ttatatttag ctagtcatta tgaaaagttg
     3841 aagggtagtc cagaagataa cgaacaaaaa caattgtttg tggagcagca taagcattat
     3901 ttagatgaga ttattgagca aatcagtgaa ttttctaagc gtgttatttt agcagatgcc
     3961 aatttagata aagttcttag tgcatataac aaacatagag acaaaccaat acgtgaacaa
     4021 gcagaaaata ttattcattt atttacgttg acgaatcttg gagctcccgc tgcttttaaa
     4081 tattttgata caacaattga tcgtaaacga tatacgtcta caaaagaagt tttagatgcc
     4141 actcttatcc atcaatccat cactggtctt tatgaaacac gcattgattt gagtcagcta
     4201 ggaggtgact aataaaagct tgatcagatc ccagcttttt tttgaagtgc ctggcagtaa
     4261 gttgaaaaaa tttgaaataa acttggacta tgttaataaa aaaatacaaa ttaacgaaca
     4321 aaaagatttg aattattata taatttagtt aaaagcaatt ttaaatgagg gaggtttcca
     4381 tatgttacgc agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttagg
     4441 tggctcaagt atgggcatca ttcgcacatg taggctcggc cctgaccaag tcaaatccat
     4501 gcgggctgct cttgatcttt tcggtcgtga gttcggagac gtagccacct actcccaaca
     4561 tcagccggac tccgattacc tcgggaactt gctccgtagt aagacattca tcgcgcttgc
     4621 tgccttcgac caagaagcgg ttgttggcgc tctcgcggct tacgttctgc ccaagtttga
     4681 gcagccgcgt agtgagatct atatctatga tctcgcagtc tccggcgagc accggaggca
     4741 gggcattgcc accgcgctca tcaatctcct caagcatgag gccaacgcgc ttggtgctta
     4801 tgtgatctac gtgcaagcag attacggtga cgatcccgca gtggctctct atacaaagtt
     4861 gggcatacgg gaagaagtga tgcactttga tatcgaccca agtaccgcca cctaatgacg
     4921 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcatgacca aaatccctta
     4981 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5041 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5101 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5161 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     5221 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5281 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5341 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5401 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     5461 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5521 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5581 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5641 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcact
     5701 agatccccgg caagttcatc cttagggttt tcatatctct taaaaagtca atttctgctt
     5761 gattaatttc ttgtggatta ttgtttttgc ggtttttcat tttttatctc cttgagtatt
     5821 gaattttttc ttatatataa atatatagca aaaactattt ttgccaactt ttttacaaaa
     5881 aattttttat taaaacactt agggctttgc taaattctct tttaaaagaa cttagtaaag
     5941 ccctatggat ttaagaactg attatactta cgtagtaaaa atactacaga tattaatagt
     6001 atagaccata tcaaaatcat aatcaattta tatttatcta aaattgtagt ccatacaaaa
     6061 tacaaaaaaa ttaggttttt ctaaattcta ttttaaaaga acttagtaac acctaatctc
     6121 taaattactt acaaaattct aaaattagat ttttagttct tcatatttct ttaaaagttt
     6181 tttaagaaaa tctttttgat tttcaaaaat ttcatgcatc ataaaactag taaatttgga
     6241 tttttttttg taaaaatcat aactttcttg ggttttaagt tgaaatctta atggttttat
     6301 tggattttgc ctagattttt ttaacgccgg actttcttta tcttttataa aattataaga
     6361 atttttaata ccattttcta ttaaataatc ttcttttata agcccctctt gcagtgcagt
     6421 tgctaatctt aaatacttat acgcttgagt ttttgctaca tcaaagtctt ttataaaatc
     6481 aagaaaagtt ttataaccat caaatttata atattgatta tcttttattt cttttaaaat
     6541 tttaatattt tccaccctgt ggaatatttc ttttttgaaa ttgtcattta acttttcctt
     6601 tagtgtcttg taataagaaa ttttactttc attaatcatt aaattttgtt caacataagt
     6661 atctatcttt tttgttaaaa ctttaacaac acgttttttt tccataaaaa ttttccttta
     6721 tttttgcaac attttcctta attgatataa ttgttactat tttagatttt ttaatatcca
     6781 cttaagaatt aatccctcac tagaatattt tttattatag aattcatata catgtttttc
     6841 taaatctata aatttatcaa caattgcttt attatagggt gtatctatct tttcttttct
     6901 taataatcgt ctcatattct ttaaataaca acaaacactg ccggttttaa acctaaattc
     6961 tatgtaatat atttttgaaa aagtatatac attttcaacc ccgtttattt catactttac
     7021 aaaaacatta tttattggtt ttctataacc atagtaaatg cctaaaaatt tgtccccgtc
     7081 tttcatagga tatagataaa gtttttgaat ytctgattta tctaataatt tttcaaaaaa
     7141 aattatacac cgcttttgtt tgttactaac tactctatgc aatgccttta atatttttgt
     7201 gtgatatata tttttatcac cttttttttc tttcagcagg aagatacttt cttttggaaa
     7261 tttaattttt tgttcattta ttcttgcagc cttaagtccg attttcatat ttaatcctta
     7321 tatagccttt ctatttaatt cttttaaact caataaactt ttttggtttt tcattagttc
     7381 taataattcg caataatatg tgcaaaacac tttattgtat tctattttct tttggttatt
     7441 caaatattct tttataattg gctttagaag ttcaatattt acttcttttt ttagttgttc
     7501 aataaggata ttgaaaatgt ttgtttttaa atcttgataa ttttgttgag aattttcttt
     7561 ttttctttca attgacttct ctaatttacg ctttatattg tttaaatcgc tatatttatg
     7621 actttcaata ataaaatgag gcttaaattt gtaattttcg tatatttttt ggaaacttgt
     7681 ttctaattgt ttagaattat attcattttt ttcaaattct ttttgagtgt tgcatagaat
     7741 ttcctttaat ttttcttgtt tttctttcat acaagattta gtaaaatgta tatttttatt
     7801 ttttagtaat ttaatttcta ttatttttat gattttaagt attttgatta tattatcttt
     7861 atcaatattt aaattgaata aaattgtaag aaaaattgaa agaatttttt tacataaaaa
     7921 gttacattta ttgaagtaat ttcttacttg atacttttct atttcattaa tctttctttc
     7981 ttcttttata ttatttttat tatttaaaca ctcctttaaa tctacactcc catttttagt
     8041 aaatctatct ttaaggcccc ttgaaactct attttgaaat cttaaatctt ttttttcttg
     8101 aaagtattta tagatttttt ggtaacattc tttttttgaa aaattaagtt gatagtatat
     8161 ttcggtcccc aaatttaccc ctaagtgttt gtgataatta gttgtgactt taaattcttt
     8221 ttctaatttg taaaggtaat tttgcagtgt ttttagttta acgggtgttt gactatttct
     8281 tttaaggttt tcattaaagt aatagagtat gtttttttgt gtgtatttct tatatgtttt
     8341 atttatgtac gctagtgttg agataaggac tactaattta tactgtaatt tattgtagca
     8401 agttgtggtt ttactggttt tgtttatatt agataagttt tccatttata gctccttgaa
     8461 taccggattt tttatctata aagtatatat atcacaattt tgatttaaaa gtaaattctt
     8521 tttttaaaaa tacaaaaatt ttttataaat tatatagacc atgatattgt attaatacta
     8581 attgatttta atttaaggtt gaactaaaat aaacatagtt ttgtagaaaa taatttttca
     8641 ttattttgct acttgatatt ggatcgtaaa aatattaggg ctttacaaag ttcttttaaa
     8701 agagaattta gtaaagccct aagtctttta ataaaaaatt ttttgtaaaa aagttggcaa
     8761 aaatagtttt tgctatatat ttatatataa gaaaaaattc aatactcaag gagataaaaa
     8821 atggaaaact taaacaataa tccacaaaaa attaatcaaa taaataagca aactaatttt
     8881 attggttttg aaaattttat agaacttgat aatcaagtta atcaaactaa agaaaaaagt
     8941 aaattaagta agataggtaa gaaattaccc ggaataagca gtcaagtcta gtgcggccgc
     9001 atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa
     9061 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
     9121 ggcagtcgcc ctaaaacaaa gttaaacatc atgaggaggt aacgtcatgg taaaagattg
     9181 gattcctatt tctcatgata attataaaca agtacaagga ccattttatc atggaactaa
     9241 agctaattta gcaattggtg atttactaac tacaggattt atttctcatt ttgaagatgg
     9301 tcgaattctt aaacatattt atttttcagc tttaatggaa ccagcagttt ggggagctga
     9361 acttgctatg tcactatctg gtcttgaagg tcgtggttat atatatatag ttgaaccaac
     9421 aggaccattt gaagatgatc caaatcttac aaataaaaaa tttcctggta atccaacaca
     9481 atcttataga acttgtgaac ctttaagaat tgttggtgtt gttgaagatt gggaaggaca
     9541 tcctgttgaa ttaataagag gaatgttaga ttcattagaa gatttaaaac gtcgtggttt
     9601 acatgttatt gaagattaac ctggcgcgcc aaataatgcc cgtgtccctg ttgaataggg
     9661 acacggtttt ttaacaattt ttttattcct aggccttagt ggccggtacc cggggatcct
     9721 tattactggc cgctttctag cctggatact tgtctggcta gttgcattag agagtcagct
     9781 agagccctag ggctagctgt ttgtgtattc ggagccagtg tagtttttct ttttactaac
     9841 gatacgggta ataattgatt gccttttaca gcctgacctt gagacaattg caaaagtctg
     9901 tccacagagg tttgacctaa taatctaaaa tcttgtttga tagtagtaag tggaggtatg
     9961 tagcaactag aatcttctgt atcatcataa cccacaactg aaatatcagc cccaactcta
    10021 agtccacttt cagtaatagc tcgcatagct cctaaagcca tttgatcatt agcaactaac
    10081 atagcagtag ggacaatgcc ttcatttaac atttgcattg tttgctgaaa tcctgacata
    10141 gcagaccaat ctccttctct ttctgctatt ggttgtattt gatttcttgt taaatacttg
    10201 tgccatcctg ctaatctcaa tctggcactt actgaactta agggacctgc aagtagtgca
    10261 atttgttgat ggcctaatgc aacaaggtgt tcaactccta accttgttcc atcttcgtga
    10321 gaaaaaatta tagagtttat tggggtttgg tccgacacat ccaaaaacaa tgctggaaca
    10381 ttagtacatg ctgcttcaac tgcaatcgca tcttggtcat caagaggata attaattata
    10441 agtccactaa cacgttgagc taataaatta tgtactgctg ccttacaagc ctctacacct
    10501 gatctctcaa ccattgatac aacaacgctt gcaccaagtt gatcagctct cgatttaatt
    10561 gcggctacaa tttgactagg cgcatgtaat gcaagtgatg atgtggcaac tccaattaaa
    10621 agtgattgtt tccccgcaag ttgctgagca acgcgatttg gtatataatt caactctgcc
    10681 atagctgctt caaccttctc tcttgttttt gcagatacat gacttgcttg gttcacaact
    10741 ctcgaaaccg tttggtagct aaccccggca tattcagcaa catcatacaa agtaacaggt
    10801 ttcatggatc attcctccat gataaaattt aaatttctga ctttggcaaa tccttttgcc
    10861 tgttaaaaag aatagcacaa aaattaaaaa aaaagaaacg caaatagcaa tagcattaaa
    10921 aataattatt attatttatt tctttgatta aaatcaaaag cctttaatgt attttcttga
    10981 tctttcatgc gcttaacggt aaaatccaag gtattaagtt caccttccac ttttctctct
    11041 ctatcttctc tagaaaa
//
