LOCUS       sequence-30862-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  TITLE     Guan lab plasmids
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HA-TSC2
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             91..180
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tgccatggcc tacccatacg atgttcctga ctatgcgggc
      121 tatccctatg acgtcccgga ctatgcagga tcctatccat atgacgttcc agattacgct
      181 gctcagtgcg gccgcatggc caaaccaact agcaaagatt caggtttgaa ggagaagttc
      241 aagatactat taggactggg aacgtcaagg ccaaatccta ggtgtgcaga aggcaaacag
      301 acggagttta tcatcacagc ggaaatcttg agagaactga gtggtgaatg cggcctcaac
      361 aatcgaatcc ggatgatagg gcagatctgt gatgtggcaa aaactaagaa acttgaagag
      421 catgcagtgg aggcactttg gaaggctgtc tcagacttgc tacagccaga gcggccaccg
      481 gaggcccgac atgcagttct ggccttgttg aaggccattg tgcagggaca gggtgatcgt
      541 ttgggggttc tccgagccct cttcttcaaa gtgatcaagg actatccttc caacgaagac
      601 ctccatgaaa ggctagaggt ttttaaggcc ctcacagaca atggaaggca tattacctac
      661 ttggaagaag aactggcaga gtttgtcctg cagtggatgg atgttggctt gtcctcagaa
      721 ttccttctgg tactcgtgaa cctggtcaag ttcaacagct gctaccttga cgaatacatt
      781 gcgccaatgg ttcacatgat ttgtctgtta tgtatccgga cagtgtcctc tgtggacatc
      841 gaggtgtcct tgc
//
