LOCUS       sequence-30863-e         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  TITLE     Guan lab plasmids
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TSC2-fl
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttggtaccg agctcggatc cactagtaac ggccgccagt
      121 gtgctggaat tctgcagata tccatcacac tggcggccgc gtcgggggtg tgcgcccttc
      181 cctgggtgca ggaggacctg cgcgcgaaag gcgcgcgcgg gagcagttct ctagtactgc
      241 cctgcccacc atggccaaac caacaagcaa agattccggc ttgaaggaga agttcaagat
      301 actgttggga ttgggaacat cgaggccaaa tcccaggtgt gcagaaggca aacagactga
      361 gtttatcatc acatcggaaa tcttgagaga actgagtggt gaatgcggcc tcaacaatcg
      421 catccgaatg atagggcaga tctgtgatgt ggcaaaaact aagaagcttg aggagcatgc
      481 agtggaggca ctttggaagg ctgtctcaga cttgctacag ccagagcggc caccagaggc
      541 ccggcatgca gttctcacct tattgaaggc cattgtacag ggacagggtg atcgtttggg
      601 agttctcaga gccctcttct tcaaagtgat caaggattac ccctccaatg aagacctcca
      661 tgaaaggcta gaagttttta aggccctcac agacaatg
//
