LOCUS       sequence-308634-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Fzd4 subtype NGS Wnt in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Miao Y, Ha A, de Lau W, Yuki K, Santos AJM, You C, Geurts MH, 
            Puschhof J, Pleguezuelos-Manzano C, Peng WC, Senlice R, Piani C, 
            Buikema JW, Gbenedio OM, Vallon M, Yuan J, de Haan S, Hemrika W, 
            Rosch K, Dang LT, Baker D, Ott M, Depeille P, Wu SM, Drost J, Nusse 
            R, Roose JP, Piehler J, Boj SF, Janda CY, Clevers H, Kuo CJ, Garcia 
            KC
  TITLE     Next-Generation Surrogate Wnts Support Organoid Growth and 
            Deconvolute Frizzled Pleiotropy In Vivo.
  JOURNAL   Cell Stem Cell. 2020 Aug 14. pii: S1934-5909(20)30358-1. doi: 
            10.1016/j.stem.2020.07.020.
  PUBMED    32818433
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2020 Aug 14. pii: S1934-5909(20)30358-1. doi: 
            10.1016/j.stem.2020.07.020."
COMMENT     Sequence Label: pHR-Fzd4 subtype NGS Wnt
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             925..942
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgaagacga tcatcgccct gagctacatc ttctgcctgg tgttcgccaa aatgtatcac
       61 actaagggac aggaaggcag cgtgtgcctg aggtcctctg actgtgcatc tggcctgtgc
      121 tgcgccagac acttttggtc caaaatctgc aagccggttc tgaaagaggg ccaggtgtgc
      181 acaaagcatc gacgaaaggg cagccatgga ctcgagatct ttcagaggtg ctactgcggg
      241 gagggtctgt cttgcagaat ccagaaagat catcatcagg cctccaactc ctcccgcctg
      301 cacacctgcc agcgacatgg aagcggttcc ggaggttctg gctccggatc tgaactgggt
      361 aaacgtctga tccgtgcagc acttgacggt aacaaagacc gtgttaaaga cctcattgaa
      421 aatggtgctg acgttaacgc gtccctgatg tctgggacga ctccgttata tgctgccgcc
      481 atgaacggcc acaaagaggt tgtgaagtta ctgatctcca agggcgcaga tgtgaatgct
      541 cagtccgttg cgggttctac acctctggtt gcggcggcga atttcggaca taacgaagtg
      601 gtaaaactgc tgataagtaa aggagcagac gtcaatgctg tgactgcgtt tggagtgacc
      661 cccctacatg ctgctgctgc tgatgggcac aaggaagtag tgaagttgct tatttctaag
      721 ggggccgacg taaatgcgaa agctggtcgt ggcatgactc cactccatat tgcagcattc
      781 cgcggtcata aggaagtcgt taaactatta atcagcaaag gtgcggattt gaacacctct
      841 gccaaagacg gtgcaacccc gcttgacatg gcgcgtgaat ctggcaatga ggaggttgtc
      901 aagctcttgg aaaagcaact cgagcaccac caccaccacc actga
//
