LOCUS       sequence-308635-        1707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Fc-tagged Fzd7/8 subtype NGS Wnt in mammalian cells for 
            organoid research.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Miao Y, Ha A, de Lau W, Yuki K, Santos AJM, You C, Geurts MH, 
            Puschhof J, Pleguezuelos-Manzano C, Peng WC, Senlice R, Piani C, 
            Buikema JW, Gbenedio OM, Vallon M, Yuan J, de Haan S, Hemrika W, 
            Rosch K, Dang LT, Baker D, Ott M, Depeille P, Wu SM, Drost J, Nusse 
            R, Roose JP, Piehler J, Boj SF, Janda CY, Clevers H, Kuo CJ, Garcia 
            KC
  TITLE     Next-Generation Surrogate Wnts Support Organoid Growth and 
            Deconvolute Frizzled Pleiotropy In Vivo.
  JOURNAL   Cell Stem Cell. 2020 Aug 14. pii: S1934-5909(20)30358-1. doi: 
            10.1016/j.stem.2020.07.020.
  PUBMED    32818433
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2020 Aug 14. pii: S1934-5909(20)30358-1. doi: 
            10.1016/j.stem.2020.07.020."
COMMENT     Sequence Label: pEGBacmam-NGS Wnt-Fc
FEATURES             Location/Qualifiers
     source          1..1707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             955..975
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1687..1704
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt
       61 gacgcgagct ctgaactggg tacgcgtctg atcagggcag cattagacgg taacaaagac
      121 cgtgttaaag acctcattga aaatggtgct gacgttaacg cgtcccttat gtctggggcg
      181 actccgttac acgccgccgc catgaacggc cacaaagagg ttgtgaagtt actgatctcc
      241 aagggcgcag atgtgaatgc tcagtccgtt gcgggttcta cacctctgga tgcggcggcg
      301 tttagtggac ataaagaagt ggtaaaactg ctgataagta aaggagcaga cgtcaatgct
      361 gttaatgcgg ctggattgac ccccctacat gctgctgctg ataatgggca caaggaagta
      421 gtgaagttgc ttatttctaa gggggccgac gtaaatgcga aagctgacca tggcatgact
      481 ccactccatt tcgcagcaca gcgcggtcat aaggaagtcg ttaaactatt aatcagcaaa
      541 ggtgcggatt tgaacacctc tgccaaagac ggtgcaaccc cgcttgacat ggcgcgtgaa
      601 tctggcaatg aggaggttgt caagctcttg gaaaagcaac tcgagggaag cggttccgga
      661 ggttctggct ccggaaaaat gtatcacact aagggacagg aaggcagcgt gtgcctgagg
      721 tcctctgact gtgcatctgg cctgtgctgc gccagacact tttggtccaa aatctgcaag
      781 ccggttctga aagagggcca ggtgtgcaca aagcatcgac gaaagggcag ccatggactc
      841 gagatctttc agaggtgcta ctgcggggag ggtctgtctt gcagaatcca gaaagatcat
      901 catcaggcct ccaactcctc ccgcctgcac acctgccagc gacatgcggc cgcagagaac
      961 ctgtacttcc agggatcttc cgagcccaaa tcttgtgaca aaactcacac atgcccaccg
     1021 tgcccagcac ctgaactcct ggggggaccg tcagtcttcc tcttcccccc aaaacccaag
     1081 gacaccctca tgatctcccg gacccctgag gtcacatgcg tggtggtgga cgtgagccac
     1141 gaagaccctg aggtcaagtt caactggtac gtggacggcg tggaggtgca taatgccaag
     1201 acaaagccgc gggaggagca gtacaacagc acgtaccgtg tggtcagcgt cctcaccgtc
     1261 ctgcaccagg actggctgaa tggcaaggag tacaagtgca aggtctccaa caaagccctc
     1321 ccagccccca tcgagaaaac catctccaaa gccaaagggc agccccgaga accacaggtg
     1381 tacaccctgc ccccatcccg ggatgagctg accaagaacc aggtcagcct gacctgcctg
     1441 gtcaaaggct tctatcccag cgacatcgcc gtggagtggg agagcaatgg gcagccggag
     1501 aacaactaca agaccacgcc tcccgtgctg gactccgacg gctccttctt cctctacagc
     1561 aagctcaccg tggacaagag caggtggcag caggggaacg tcttctcatg ctccgtgatg
     1621 catgaggctc tgcacaacca ctacacgcag aagagcctct ccctgtctcc gggtaaaggg
     1681 gccgcacatc accatcacca tcactaa
//
