LOCUS       sequence-308636-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Fzd7/8 subtype NGS Wnt in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Miao Y, Ha A, de Lau W, Yuki K, Santos AJM, You C, Geurts MH, 
            Puschhof J, Pleguezuelos-Manzano C, Peng WC, Senlice R, Piani C, 
            Buikema JW, Gbenedio OM, Vallon M, Yuan J, de Haan S, Hemrika W, 
            Rosch K, Dang LT, Baker D, Ott M, Depeille P, Wu SM, Drost J, Nusse 
            R, Roose JP, Piehler J, Boj SF, Janda CY, Clevers H, Kuo CJ, Garcia 
            KC
  TITLE     Next-Generation Surrogate Wnts Support Organoid Growth and 
            Deconvolute Frizzled Pleiotropy In Vivo.
  JOURNAL   Cell Stem Cell. 2020 Aug 14. pii: S1934-5909(20)30358-1. doi: 
            10.1016/j.stem.2020.07.020.
  PUBMED    32818433
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2020 Aug 14. pii: S1934-5909(20)30358-1. doi: 
            10.1016/j.stem.2020.07.020."
COMMENT     Sequence Label: RKS-Fzd7/8 subtype NGS Wnt
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             940..963
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 atgaagacca tcatcgccct gagctacatc ttctgcctgg tgttcgccgg atcctctgaa
       61 ctgggtacgc gtctgatcag ggcagcatta gacggtaaca aagaccgtgt taaagacctc
      121 attgaaaatg gtgctgacgt taacgcgtcc cttatgtctg gggcgactcc gttacacgcc
      181 gccgccatga acggccacaa agaggttgtg aagttactga tctccaaggg cgcagatgtg
      241 aatgctcagt ccgttgcggg ttctacacct ctggatgcgg cggcgtttag tggacataaa
      301 gaagtggtaa aactgctgat aagtaaagga gcagacgtca atgctgttaa tgcggctgga
      361 ttgacccccc tacatgctgc tgctgataat gggcacaagg aagtagtgaa gttgcttatt
      421 tctaaggggg ccgacgtaaa tgcgaaagct gaccatggca tgactccact ccatttcgca
      481 gcacagcgcg gtcataagga agtcgttaaa ctattaatca gcaaaggtgc ggatttgaac
      541 acctctgcca aagacggtgc aaccccgctt gacatggcgc gtgaatctgg caatgaggag
      601 gttgtcaagc tcttggaaaa gcaactcgag ggaagcggtt ccggaggttc tggctccgga
      661 aaaatgtatc acactaaggg acaggaaggc agcgtgtgcc tgaggtcctc tgactgtgca
      721 tctggcctgt gctgcgccag acacttttgg tccaaaatct gcaagccggt tctgaaagag
      781 ggccaggtgt gcacaaagca tcgacgaaag ggcagccatg gactcgagat ctttcagagg
      841 tgctactgcg gggagggtct gtcttgcaga atccagaaag atcatcatca ggcctccaac
      901 tcctcccgcc tgcacacctg ccagcgacat gcggccgcgc atcatcacca ccatcaccac
      961 cattaa
//
