LOCUS       sequence-30864-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  TITLE     Guan lab plasmids
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Rheb WT
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..31
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        241..259
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     241..258
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     272..291
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             340..369
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
       61 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
      121 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
      181 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
      241 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
      301 caggtccaac tgcacctcgg ttctatcgat cccaccatgg agcagaagct gatcagcgag
      361 gaggacctgc tcgagggatc catgccgcag tccaagtccc ggaagatcgc gatcctgggc
      421 taccggtctg tggggaaatc ctcattgacg attcaatttg ttgaaggcca atttgtggac
      481 tcctacgatc caaccataga aaacactttt acaaagttga tcacagtaaa tggacaagaa
      541 tatcatcttc aacttgtaga cacagccggg caagatgaat attctatctt tcctcagaca
      601 tactccatag atattaatgg ctatattctt gtgtattctg ttacatcaat caaaagtttt
      661 gaagtgatta aagttatcca tggcaaattg ttggatatgg tggggaaagt acaaatacct
      721 attatgttgg ttgggaataa gaaagacctg catatggaaa gggtgatcag ttatgaagaa
      781 gggaaagctt tggcaganct tggaatgcag cctttttgga atcttctgct aaagaaaatc
//
