LOCUS       sequence-308646-       11127 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast integrating plasmid to express MCP-ABI and PYL1-VP64.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11127)
  AUTHORS   Cunningham-Bryant D, Sun J, Fernandez B, Zalatan JG
  TITLE     CRISPR-Cas-Mediated Chemical Control of Transcriptional Dynamics in 
            Yeast.
  JOURNAL   Chembiochem. 2019 Jun 14;20(12):1519-1523. doi: 
            10.1002/cbic.201800823. Epub 2019 Apr 26.
  PUBMED    30710419
REFERENCE   2  (bases 1 to 11127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/cbic.201800823"; journalName: "Chembiochem"; date: 
            "2019-06-14- 14"; volume: "20"; issue: "12"; pages: "1519-1523"
COMMENT     Sequence Label: pDCB014
FEATURES             Location/Qualifiers
     source          1..11127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1964..1984
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        4288..4992
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             5008..5028
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5614..5763
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     primer_bind     complement(7074..7093)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7075..7093)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7100..7117)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7100..7116)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7109..7131)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7257..7712
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7344..7363)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7554..7575
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8068..8087)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8187..8209
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8247..8265)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8350..8454
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8455..8523,8524..9315)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8673..8692)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9486..10074
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9975..9994
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10228..10245
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10362..10383
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10398..10403,10404..10421,10422..10428)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10436..10452
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10441..10463
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10460..10476
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10460..10476
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        10497..10515
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10841..11028
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
ORIGIN
        1 agcatcgagc aaaggatggc gtttgttaag agggttacgc aggagacgaa tatacagctg
       61 gcgctggatc ttgacggtgg gtctgtttct gtacgggaga gcatactggg caaggaatat
      121 gctagtggtg atgggcagac catccatgtg cacactggag ttgggttttt ggaccacatg
      181 ttgactgcgc tggcgaagca tggcgggtgg tctctgatcc tggagtgtat aggggacttg
      241 cacatcgatg accaccacac tgttgaggac tgtgggattg cgctgggcca agcgttcaag
      301 gaggcgcttg gctccgtgcg tggtatcaag aggttcgggc atgggtttgc accactggac
      361 gaggcgctga gccgcgctgt ggttgacttc tccaataggc ctttcgccgt ggtggagctg
      421 ggcctgaagc gagaacgcat aggccagcta tccacagaga tgatcccgca cttcttggag
      481 agtttcgcca ctgaggcgcg tatcaccatg catgtggact gtctgcgggg caccaacgac
      541 caccaccgct ccgaatcagc tttcaaggcg ctcgccatcg ccatcagaga ggcaagaaca
      601 cctacgggtc gcgatgacgt tccatccact aagggtgtcc tagcatagaa cacagcccac
      661 agctaccacc atcaacaata tttatataga taacgtacac atagaaatca cacaaacaga
      721 gtatttattc ttaactacat gaactaccat cagaccgtct gggccactat ataatgtgct
      781 attcataaac gtgatcactc tacgtagcag gcgaccccag gtgaaaattt ttcagcgagc
      841 tgccagattg tcaggtgaaa aactgaaaaa aacttctggg cgatgagctt gtggcgggaa
      901 attaagtata taaagcagtt agtttcttct gcttcttgtg gttctgggta ttcttgtaat
      961 atttggaata atagaaaacc aagactactg cctttctttt catattatag aggaagaaat
     1021 acgcacgaac acgatataga ggtaaatttt ttgcaaaaag ataccaaact ataaaagaaa
     1081 tagaagacta aactacaaaa aataaagaaa gtaatacata tatccaatat gactgagtta
     1141 gcagagcaag tttccatgcc tgaagggcaa caaagacgtg gtggttacgt tccacctcac
     1201 atccgtaacg gtggtggcag aagaggcgat tctgctcctc gtggtggttt caacaacggt
     1261 ggcggccgtg gtggtttctt cagaaacaac ggtgaccgtg gtggattcgg ctttgggtac
     1321 cgggcccaaa gtggaagtca caaaacagtg gtcctatata aaagaacaag aaaaagatta
     1381 tttatataca actgcggtca caagaagcaa cgcgagagag cacaacacgc tgttatcacg
     1441 caaactatgt tttgacaccg agccatagcc gtgattgtgc gtcacattgg gcgataatga
     1501 acgctaaatg accaactccc atccgtagga gccccttagg gcgtgccaat agtttcacgc
     1561 gcttaatgcg aagtgctcgg aacggacaac tgtggtcgtt tggcaccggg aaagtggtac
     1621 tagaccgaga gtttcgcatt tgtatggcag gacgttctgg gagcttcgcg tctaaagctt
     1681 tttcgggcgc gaaatgcaga ccagaccaga acaaaacaac tgacaagaag gcgtttaatt
     1741 taatatgttg ttcactcgcg cctgggctgt tgttattcgg ctagatacat acgtgtttgt
     1801 gcgtatgtag ttatatcata tataagtata ttaggatgag gcggtgaaag agattttttt
     1861 tttttcgctt aatttattct tttctctatc ttttttccta catcttgttc aaaagagtag
     1921 caaaaacaac aatcaataca ataaaataat gtctctcgag atgcccaaaa agaaaagaaa
     1981 agtgggtagt atggcttcta actttactca gttcgttctc gtcgacaatg gcggaactgg
     2041 cgacgtgact gtcgccccaa gcaacttcgc taacgggatc gctgaatgga tcagctctaa
     2101 ctcgcgttca caggcttaca aagtaacctg tagcgttcgt cagagctctg cgcagaatcg
     2161 caaatacacc atcaaagtcg aggtgcctaa aggcgcctgg cgttcgtact taaatatgga
     2221 actaaccatt ccaattttcg ccacgaattc cgactgcgag cttattgtta aggcaatgca
     2281 aggtctccta aaagatggaa acccgattcc ctcagcaatc gcagcaaact ccggcatcta
     2341 cggctccacg cgtgtgcctt tgtatggttt tacttcgatt tgtggaagaa gacctgagat
     2401 ggaagctgct gtttcgacta taccaagatt ccttcaatct tcctctggtt cgatgttaga
     2461 tggtcggttt gatcctcaat ccgccgctca tttcttcggt gtttacgacg gccatggcgg
     2521 ttctcaggta gcgaactatt gtagagagag gatgcatttg gctttggcgg aggagatagc
     2581 taaggagaaa ccgatgctct gcgatggtga tacgtggctg gagaagtgga agaaagctct
     2641 tttcaactcg ttcctgagag ttgactcgga gattgagtca gttgcgccgg agacggttgg
     2701 gtcaacgtcg gtggttgccg ttgttttccc gtctcacatc ttcgtcgcta actgcggtga
     2761 ctctagagcc gttctttgcc gcggcaaaac tgcacttcca ttatccgttg accataaacc
     2821 ggatagagaa gatgaagctg cgaggattga agccgcagga gggaaagtga ttcagtggaa
     2881 tggagctcgt gttttcggtg ttctcgccat gtcgagatcc attggcgata gatacttgaa
     2941 accatccatc attcctgatc cggaagtgac ggctgtgaag agagtaaaag aagatgattg
     3001 tctgattttg gcgagtgacg gggtttggga tgtaatgacg gatgaagaag cgtgtgagat
     3061 ggcaaggaag cggattctct tgtggcacaa gaaaaacgcg gtggctgggg atgcatcgtt
     3121 gctcgcggat gagcggagaa aggaagggaa agatcctgcg gcgatgtccg cggctgagta
     3181 tttgtcaaag ctggcgatac agagaggaag caaagacaac ataagtgtgg tggtggttga
     3241 tttgaaggcg gccgccaccg cggtggagct ctaaagtgct tttaactaag aattattagt
     3301 cttttctgct tattttttca tcatagttta gaacacttta tattaacgaa tagtttatga
     3361 atctatttag gtttaaaaat tgatacagtt ttataagtta ctttttcaaa gactcgtgct
     3421 gtctattgca taatgcactg gaaggggaaa aaaaaggtgc acacgcgtgg ctttttcttg
     3481 aatttgcagt ttgaaaaatg gccctaaaac aagaagaggg ttgactacat cacgatgagg
     3541 gggatcgaag aaatgatggt aaatgaaata ggaaatcaag gagcatgaag gcaaaagaca
     3601 aatataaggg tcgaacgaaa aataaagtga aaagtgttga tatgatgtat ttggctttgc
     3661 ggcgccgaaa aaacgagttt acgcaattgc acaatcatgc tgactctgtg gcggacccgc
     3721 gctcttgccg gcccggcgat aacgctgggc gtgaggctgt gcccggcgga gttttttgcg
     3781 cctgcatttt ccaaggttta ccctgcgcta aggggcgaga ttggagaagc aataagaatg
     3841 ccggttgggg ttgcgatgat gacgaccacg acaactggtg tcattattta agttgccgaa
     3901 agaacctgag tgcatttgca acatgagtat actagaagaa tgagccaaga cttgcgagac
     3961 gcgagtttgc cggtggtgcg aacaatagag cgaccatgac cttgaaggtg agacgcgcat
     4021 aaccgctaga gtactttgaa gaggaaacag caatagggtt gctaccagta taaatagaca
     4081 ggtacataca acactggaaa tggttgtctg tttgagtacg ctttcaattc atttgggtgt
     4141 gcactttatt atgttacaat atggaaggga actttacact tctcctatgc acatatatta
     4201 attaaagtcc aatgctagta gagaaggggg gtaacacccc tccgcgctct tttccgattt
     4261 ttttctaaac cgtggaatat ttcggatatc cttttgttgt ttccgggtgt acaatatgga
     4321 cttcctcttt tctggcaacc aaacccatac atcgggattc ctataatacc ttcgttggtc
     4381 tccctaacat gtaggtggcg gaggggagat atacaataga acagatacca gacaggacat
     4441 aatgggctaa acaagactac accaattaca ctgcctcatt gatggtggta cataacgaac
     4501 taatactgta gccctagact tgatagccat catcatatcg aagtttcact accctttttc
     4561 catttgccat ctattgaagt aataataggc gcatgcaact tcttttcttt ttttttcttt
     4621 tctctctccc ccgttgttgt ctcaccatat ccgcaatgac aaaaaaatga tggaagacac
     4681 taaaggaaaa aattaacgac aaagacagca ccaacagatg tcgttgttcc agagctgatg
     4741 aggggtatct cgaagcacac gaaacttttt ccttccttca ttcacgcaca ctactctcta
     4801 atgagcaacg gtatacggcc ttccttccag ttacttgaat ttgaaataaa aaaaagtttg
     4861 ctgtcttgct atcaagtata aatagacctg caattattaa tcttttgttt cctcgtcatt
     4921 gttctcgttc cctttcttcc ttgtttcttt ttctgcacaa tatttcaagc tataccaagc
     4981 atacaatcaa ctatctcata tacaatgccc aaaaagaaaa gaaaagtgat gggtgggggc
     5041 gcgccaactc aagacgaatt cacccaactc tcccaatcaa tcgccgagtt ccacacgtac
     5101 caactcggta acggccgttg ctcatctctc ctagctcagc gaatccacgc gccgccggaa
     5161 acagtatggt ccgtggtgag acgtttcgat aggccacaga tttacaaaca cttcatcaaa
     5221 agctgtaacg tgagtgaaga tttcgagatg cgagtgggat gcacgcgcga cgtgaacgtg
     5281 ataagtggat taccggcgaa tacgtctcga gagagattag atctgttgga cgatgatcgg
     5341 agagtgactg ggtttagtat aaccggtggt gaacataggc tgaggaatta taaatcggtt
     5401 acgacggttc atagatttga gaaagaagaa gaagaagaaa ggatctggac cgttgttttg
     5461 gaatcttatg ttgttgatgt accggaaggt aattcggagg aagatacgag attgtttgct
     5521 gatacggtta ttagattgaa tcttcagaaa cttgcttcga tcactgaagc tatgaacgaa
     5581 ttctcaagcg ctgcaggtac tagtggacga gccgatgctc tcgacgattt tgacctcgat
     5641 atgctgggat ccgacgctct cgatgacttc gaccttgaca tgctggggag tgacgctctg
     5701 gacgacttcg atctcgacat gctgggctcc gacgccctgg atgacttcga tctggacatg
     5761 ctcatcaact aaggatccta ggcggccgcc accgcggtgg agctctaagc aaatagctaa
     5821 attatatacg aattaatatt atgattaagt gtttacgtga gtgcgatatt tttattacta
     5881 tcttatacag ttgtatatac tctataaaat gagttgtcta ttaattaacg cgatgaatgc
     5941 tttctgggtt tacctctcca acaactctag tttacttctc aatacattca attgtatttg
     6001 atttgtcaat acttcatcat taatcaattc tatagttttg tttttctcgt ttatttccaa
     6061 atttaatgca tcaattttat tattcaattt gtcgttgatt ttggttaatg attttatggt
     6121 ttgatctctg gcattgattg tttgtgttag tttttcatta ttgataatta aattatttaa
     6181 gttagttatc aactcggtgt tttcaagttt caagttttca atttctttag agtttattag
     6241 atttgtcaaa gtttctgaat tgcttgattg gtcctgtaga agagtatttg ttgttgtgga
     6301 taattgattc aatttttgag acaattgctg gaaggcgttg aaatatctag catcaatctc
     6361 atggtttttt tcccgagagt ctcgtagatt caattgtttt aatatatctt gggaccactc
     6421 ttgatttgaa ctcatggaaa ttaaactggg tgttgtgttg tggtgtaatg attgtacccc
     6481 ctttgcttat aattgtgtgg tgacaccgat tatttaaagc tgcagcatac gatatatata
     6541 catgtgtata tatgtatacc tatgaatgtc agtaagtatg tatacgaaca gtatgatact
     6601 gaagatgaca aggtaatgca tcattctata cgtgtcattc tgaacgaggc gcgctttcct
     6661 tttttctttt tgctttttct ttttttttct cttgaactcc agaaaaaaaa tataaaagag
     6721 atggaggaac gggaaaaagt tagttgtggt gataggtggc aagtggtatt ccgtaagaac
     6781 aacaagaaaa gcatttcata ttatggctga actgagcgaa caagtgcaaa atttaagcat
     6841 caacgacaac aacgagaatg gttatgttcc tcctcactta agaggaaaac caagaagtgc
     6901 cagaaataac agtagcaact acaataacaa caacggcggc tacaacggtg gccgtggcgg
     6961 tggcagcttc tttagcaaca accgtcgtgg tggttacggc aacggtggtt tcttcggtgg
     7021 aaacaacggt ggcagcggcc ggccatttaa atgtttaaac ggccacgctg gccccctata
     7081 gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     7141 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     7201 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggacgc
     7261 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     7321 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     7381 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     7441 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     7501 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     7561 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     7621 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     7681 gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat
     7741 ctgtgcggta tttcacaccg catagggtaa taactgatat aattaaattg aagctctaat
     7801 ttgtgagttt agtattagga tctctaccct tggcgaaaag tcctctgcca acaatgatga
     7861 tatctgatcc accacttaca acttcgtcga cggttctgta ctgctgaccc aatatgcgta
     7921 tatataccaa tctaagtctg tgctccttcc ttcgttcttc cttctgttcg gagattaccg
     7981 aatcaaaaaa atttcaagga aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat
     8041 tgaaaaggtg gtatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     8101 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     8161 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     8221 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     8281 catgataata atggtttctt agacgtcggc gcgccgagga cgtcaggtgg cacttttcgg
     8341 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     8401 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     8461 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     8521 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     8581 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     8641 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     8701 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     8761 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     8821 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     8881 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     8941 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     9001 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     9061 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     9121 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     9181 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     9241 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     9301 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     9361 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     9421 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     9481 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     9541 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     9601 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     9661 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     9721 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     9781 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     9841 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     9901 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     9961 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    10021 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    10081 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    10141 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    10201 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
    10261 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
    10321 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
    10381 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
    10441 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctcggaatt
    10501 aaccctcact aaagggaaca aaagctgggc cggccattta aatgtttaaa cggccacgct
    10561 ggccgctgca cggtcctgtt ccctagcatg tacgtgagcg tatttccttt taaaccacga
    10621 cgctttgtct tcattcaacg tttcccattg tttttttcta ctattgcttt gctgtgggaa
    10681 aaacttatcg aaagatgacg actttttctt aattctcgtt ttaagagctt ggtgagcgct
    10741 aggagtcact gccaggtatc gtttgaacac ggcattagtc agggaagtca taacacagtc
    10801 ctttcccgca attttctttt tctattactc ttggcctcct ctagtacact ctatattttt
    10861 ttatgcctcg gtaatgattt tcattttttt ttttccacct agcggatgac tctttttttt
    10921 tcttagcgat tggcattatc acataatgaa ttatacatta tataaagtaa tgtgatttct
    10981 tcgaagaata tactaaaaaa tgagcaggca agataaacga aggcaaagag gctctatatg
    11041 tatataaagc tgcggatgcc ttctgattta atctagttct agtttacttc aggtttactt
    11101 aaagttgaga taattgaagc aaagaga
//
