LOCUS       sequence-308665-        8923 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ZIM3 KRAB domain fused to PYL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8923)
  AUTHORS   Alerasool N, Segal D, Lee H, Taipale M
  TITLE     An efficient KRAB domain for CRISPRi applications in human cells.
  JOURNAL   Nat Methods. 2020 Nov;17(11):1093-1096. doi: 
            10.1038/s41592-020-0966-x. Epub 2020 Oct 5.
  PUBMED    33020655
REFERENCE   2  (bases 1 to 8923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0966-x"; journalName: "Nat Methods"; date: 
            "2020-11"; volume: "17"; issue: "11"; pages: "1093-1096"
COMMENT     Sequence Label: pLX301-ZIM3-KRAB-PYL1
FEATURES             Location/Qualifiers
     source          1..8923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    67..184
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        236..615
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..819
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     770..790
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        864..882
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    970..994
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1295..1319)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1970..2558
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2023..2043)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2441..2452)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2630..2863
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2941..3062
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2978..2997)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3032..3051
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3102..3237
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(3258..3276)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3286..3303)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3286..3302)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3295..3317)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3444..3899
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3531..3550)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3741..3762
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3925..4029
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4030..4098,4099..4890)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4248..4267)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5061..5649
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5550..5569
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5803..5820
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5937..5958
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5973..5978,5979..5996,5997..6003)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6011..6027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6016..6038
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6035..6051
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6035..6051
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6070..6090
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6072..6090
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6118..6344
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6345..6525
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6572..6697
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7190..7423
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7608..7652
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7827..8327
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8246..8264
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(8349..8923,1..25)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8349..8368)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8845..8865
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 catgacccgc aagcccggtg cctgagaatt ctagatcttg agacaaatgg cagtattcat
       61 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
      121 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
      181 ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctac gcgttgacat
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      421 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      781 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
      841 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
      901 cgtttaaact taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg
      961 cagatatcaa caagtttgta caaaaaagtt ggcatgaaca attcccaggg aagagtgacc
     1021 ttcgaggatg tcactgtgaa cttcacccag ggggagtggc agcggctgaa tcccgaacag
     1081 agaaacttgt acagggatgt gatgctggag aattacagca accttgtctc tgtgggacaa
     1141 ggggaaacca ccaaacccga tgtgatcttg aggttggaac aaggaaagga gccatggttg
     1201 gaggaagagg aagtgctggg aagtggccgt gcagaaaaaa atggggacat tggagggcag
     1261 atttggaagc caaaggatgt gaaagagagt ctctacccaa ctttcttgta caaagtggtt
     1321 gatatccagc acagtggcgg ccgctcgagt ggctcgggct cgacctcggg ctcgggcaaa
     1381 accggtggtg gcggagcacc aacgcaagat gaatttaccc agctcagcca aagtatcgcc
     1441 gaatttcaca cctatcaact tggaaatggt aggtgctcta gcctcttggc ccaaagaatc
     1501 catgcacccc cagaaactgt gtggtccgtg gtgagacgat ttgatagacc tcagatctac
     1561 aaacacttca taaagtcttg caacgtaagt gaggattttg agatgagggt agggtgcaca
     1621 cgcgatgtga atgttatctc tgggctcccc gccaatactt caagagagag gctggacttg
     1681 ctggacgacg accgacgggt cacgggcttc tccataacag gtggggagca tcgcctgaga
     1741 aattataaat cagttacaac ggtgcataga tttgaaaagg aagaggaaga agaacggatc
     1801 tggactgtcg tgcttgagtc ctatgtagtg gatgttcccg aagggaactc cgaggaagac
     1861 acaaggcttt tcgctgatac agtaattaga ttgaacctgc aaaagctcgc ctcaataaca
     1921 gaggccatga actaagggcc cgcgctaacc ggtggcgcgt taagtcgaca atcaacctct
     1981 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     2041 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     2101 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     2161 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     2221 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     2281 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     2341 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     2401 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     2461 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     2521 tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact
     2581 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     2641 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     2701 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     2761 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     2821 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca
     2881 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     2941 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     3001 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     3061 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     3121 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     3181 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     3241 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     3301 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     3361 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     3421 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     3481 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     3541 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     3601 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     3661 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     3721 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     3781 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     3841 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     3901 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     3961 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4021 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4081 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     4141 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     4201 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     4261 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     4321 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     4381 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     4441 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     4501 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     4561 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     4621 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     4681 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     4741 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     4801 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     4861 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     4921 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     4981 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5041 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5101 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5161 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5221 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5281 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5341 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5401 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     5461 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5521 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5581 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5641 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5701 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     5761 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     5821 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     5881 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     5941 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     6001 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     6061 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt
     6121 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
     6181 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
     6241 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
     6301 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag
     6361 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6421 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     6481 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     6541 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     6601 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     6661 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     6721 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     6781 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     6841 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     6901 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     6961 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     7021 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     7081 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     7141 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     7201 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     7261 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     7321 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     7381 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     7441 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     7501 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     7561 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     7621 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     7681 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     7741 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     7801 cccacctccc aaccccgagg ggacccttgc gccttttcca aggcagccct gggtttgcgc
     7861 agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc
     7921 gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc
     7981 ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt
     8041 gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc
     8101 agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag
     8161 ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag
     8221 tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt
     8281 cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg gtaccaccgg
     8341 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     8401 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     8461 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     8521 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca
     8581 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     8641 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     8701 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     8761 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     8821 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     8881 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtg
//
