LOCUS       sequence-308676-        8544 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid encoding two sgRNAs for targeting of 
            the CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection
            marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8544)
  AUTHORS   Umkehrer C, Holstein F, Formenti L, Jude J, Froussios K, Neumann T, 
            Cronin SM, Haas L, Lipp JJ, Burkard TR, Fellner M, Wiesner T, Zuber 
            J, Obenauf AC
  TITLE     Isolating live cell clones from barcoded populations using 
            CRISPRa-inducible reporters.
  JOURNAL   Nat Biotechnol. 2020 Jul 27. pii: 10.1038/s41587-020-0614-0. doi: 
            10.1038/s41587-020-0614-0.
  PUBMED    32719478
REFERENCE   2  (bases 1 to 8544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Jul 27. pii: 10.1038/s41587-020-0614-0. doi: 
            10.1038/s41587-020-0614-0."
COMMENT     Sequence Label: CaTCH Dual-sgRNA_sgBC1-A_sgBC1-C
FEATURES             Location/Qualifiers
     source          1..8544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             118..298
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    345..470
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    963..1196
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1381..1425
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1603..1851
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1603..1623
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1774..1793
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2044..2357
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     2276..2296
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_feature    2513..2630
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2699..2910
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2930..3415
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     CDS             3425..3481
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3476..4276
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3536..3555)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4146..4165
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    4287..4875
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4340..4360)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4758..4769)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4947..5180
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5258..5379
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5295..5314)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5349..5368
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5419..5554
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5574..5593)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5575..5593)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5603..5620)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5603..5619)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5612..5634)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5761..6216
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5848..5867)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6058..6079
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6242..6346
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6347..6415,6416..7207)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6565..6584)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7378..7966
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7867..7886
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8120..8137
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8254..8275
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8290..8295,8296..8313,8314..8320)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8328..8344
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8333..8355
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8352..8368
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8352..8368
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8387..8407
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8389..8407
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        join(8435..8544,1..117)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
       61 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
      121 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      181 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      241 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      301 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      361 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      421 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      481 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
      541 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
      601 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
      661 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
      721 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
      781 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
      841 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
      901 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
      961 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1021 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1081 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1141 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1201 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1261 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1321 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1381 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1441 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1501 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     1561 ttatcgtttc agacccacct cccaaccccg aggggaccca gagagggcct atttcccatg
     1621 attccttcat atttgcatat acgatacaag gctgttagag agataattag aattaatttg
     1681 actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg
     1741 tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga
     1801 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac cgctgtgctc
     1861 gttccacaca cgtttaagag ctatgctgga aacagcatag caagtttaaa taaggctagt
     1921 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttgaattc tagatcttga
     1981 gaactttgtc tcacctgctg ctgggaccaa gctaattata aagccacccc gaggggaccc
     2041 agagatccga cgccgccatc tctaggcccg cgccggcccc ctcgcacaga cttgtgggag
     2101 aagctcggct actcccctgc cccggttaat ttgcatataa tatttcctag taactataga
     2161 ggcttaatgt gcgataaaag acagataatc tgttcttttt aatactagct acattttaca
     2221 tgataggctt ggatttctat aagagataca aatactaaat tattatttta aaaaacagca
     2281 caaaaggaaa ctcaccctaa ctgtaaagta attgtgtgtt ttgagactat aaatatccct
     2341 tggagaaaag ccttgtttgg gcgagcgcgc gcaaatgggt ttaagagcta tgctggaaac
     2401 agcatagcaa gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
     2461 gtgctttttt tgaattctag atcttgagac aaatggcagt attcatccac aattttaaaa
     2521 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2581 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     2641 acagggacag cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg
     2701 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     2761 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     2821 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     2881 tttcgcaacg ggtttgccgc cagaacacag gtgtcgtgac gcggccacca tgaacccagc
     2941 catcagcgtc gctctcctgc tctcagtctt gcaggtgtcc cgagggcaga aggtgaccag
     3001 cctgacagcc tgcctggtga accaaaacct tcgcctggac tgccgccatg agaataacac
     3061 caaggataac tccatccagc atgagttcag cctgacccga gagaagagga agcacgtgct
     3121 ctcaggcacc ctcgggatac ccgagcacac gtaccgctcc cgcgtcaccc tctccaacca
     3181 gccctatatc aaggtcctta ccctagccaa cttcaccacc aaggatgagg gcgactactt
     3241 ttgtgagctt cgagtctcgg gcgcgaatcc catgagctcc aacaaaagta tcagtgtgta
     3301 tagagacaaa ctggtcaagt gtggcggcat aagcctgctg gttcagaaca catcctggat
     3361 gctgctgctg ctgctttccc tctccctcct ccaagccctg gacttcattt ctctgggcag
     3421 cggcgccacc aacttcagcc tgctgaagca ggccggcgac gtggaggaga accccggccc
     3481 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     3541 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     3601 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     3661 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     3721 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     3781 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     3841 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     3901 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     3961 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     4021 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     4081 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     4141 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     4201 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     4261 tgacgagttc ttctgattaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     4321 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     4381 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     4441 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     4501 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     4561 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     4621 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     4681 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     4741 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     4801 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     4861 gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag
     4921 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     4981 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     5041 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     5101 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     5161 agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt
     5221 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     5281 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5341 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     5401 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5461 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     5521 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     5581 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     5641 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     5701 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     5761 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     5821 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     5881 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     5941 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6001 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6061 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6121 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6181 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     6241 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6301 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6361 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6421 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6481 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6541 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     6601 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     6661 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     6721 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     6781 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6841 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6901 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6961 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7021 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7081 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7141 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7201 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7261 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7321 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7381 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7441 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7501 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     7561 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7621 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7681 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7741 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7801 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7861 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7921 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7981 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8041 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8101 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     8161 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     8221 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     8281 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     8341 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     8401 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
     8461 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     8521 gcatgccgat tggtggaagt aagg
//
