LOCUS       sequence-308682-        8543 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid encoding two sgRNAs without a target. 
            These can be used as a off-target control for CaTCH barcode cassette
            BC1. Expresses Thy1.1 and a Neo selection marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8543)
  AUTHORS   Umkehrer C, Holstein F, Formenti L, Jude J, Froussios K, Neumann T, 
            Cronin SM, Haas L, Lipp JJ, Burkard TR, Fellner M, Wiesner T, Zuber 
            J, Obenauf AC
  TITLE     Isolating live cell clones from barcoded populations using 
            CRISPRa-inducible reporters.
  JOURNAL   Nat Biotechnol. 2020 Jul 27. pii: 10.1038/s41587-020-0614-0. doi: 
            10.1038/s41587-020-0614-0.
  PUBMED    32719478
REFERENCE   2  (bases 1 to 8543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Jul 27. pii: 10.1038/s41587-020-0614-0. doi: 
            10.1038/s41587-020-0614-0."
COMMENT     Sequence Label: CaTCH Dual-sgRNA_sgBC2-A_sgBC2-C
FEATURES             Location/Qualifiers
     source          1..8543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    85..210
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    703..936
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1121..1165
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1343..1591
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1343..1363
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1514..1533
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        1784..2097
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     2016..2036
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_feature    2252..2369
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2438..2649
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2669..3154
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     CDS             3164..3220
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3215..4015
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3275..3294)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3885..3904
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    4026..4614
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4079..4099)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4497..4508)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4686..4919
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4997..5118
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5034..5053)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5088..5107
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5158..5293
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5313..5332)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5314..5332)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5342..5359)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5342..5358)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5351..5373)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5500..5955
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5587..5606)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5797..5818
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5981..6085
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6086..6154,6155..6946)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6304..6323)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7117..7705
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7606..7625
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7859..7876
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7993..8014
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8029..8034,8035..8052,8053..8059)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8067..8083
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8072..8094
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8091..8107
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8091..8107
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8126..8146
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8128..8146
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8174..8400
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             join(8401..8543,1..38)
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
       61 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
      121 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
      181 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
      241 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
      301 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
      361 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
      421 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
      481 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
      541 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
      601 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
      661 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
      721 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
      781 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
      841 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
      901 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
      961 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1021 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1081 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1141 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1201 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1261 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1321 cccaaccccg aggggaccca gagagggcct atttcccatg attccttcat atttgcatat
     1381 acgatacaag gctgttagag agataattag aattaatttg actgtaaaca caaagatatt
     1441 agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt
     1501 atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc
     1561 tttatatatc ttgtggaaag gacgaaacac cgtaggaaac ttcaaatacc agtttaagag
     1621 ctatgctgga aacagcatag caagtttaaa taaggctagt ccgttatcaa cttgaaaaag
     1681 tggcaccgag tcggtgcttt ttttgaattc tagatcttga gaactttgtc tcacctgctg
     1741 ctgggaccaa gctaattata aagccacccc gaggggaccc agagatccga cgccgccatc
     1801 tctaggcccg cgccggcccc ctcgcacaga cttgtgggag aagctcggct actcccctgc
     1861 cccggttaat ttgcatataa tatttcctag taactataga ggcttaatgt gcgataaaag
     1921 acagataatc tgttcttttt aatactagct acattttaca tgataggctt ggatttctat
     1981 aagagataca aatactaaat tattatttta aaaaacagca caaaaggaaa ctcaccctaa
     2041 ctgtaaagta attgtgtgtt ttgagactat aaatatccct tggagaaaag ccttgtttga
     2101 ggtctggaat atcagccgtt taagagctat gctggaaaca gcatagcaag tttaaataag
     2161 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt gaattctaga
     2221 tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     2281 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     2341 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
     2401 tttggcgccg gctcgagtgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     2461 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
     2521 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     2581 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     2641 agaacacagg tgtcgtgacg cggccaccat gaacccagcc atcagcgtcg ctctcctgct
     2701 ctcagtcttg caggtgtccc gagggcagaa ggtgaccagc ctgacagcct gcctggtgaa
     2761 ccaaaacctt cgcctggact gccgccatga gaataacacc aaggataact ccatccagca
     2821 tgagttcagc ctgacccgag agaagaggaa gcacgtgctc tcaggcaccc tcgggatacc
     2881 cgagcacacg taccgctccc gcgtcaccct ctccaaccag ccctatatca aggtccttac
     2941 cctagccaac ttcaccacca aggatgaggg cgactacttt tgtgagcttc gagtctcggg
     3001 cgcgaatccc atgagctcca acaaaagtat cagtgtgtat agagacaaac tggtcaagtg
     3061 tggcggcata agcctgctgg ttcagaacac atcctggatg ctgctgctgc tgctttccct
     3121 ctccctcctc caagccctgg acttcatttc tctgggcagc ggcgccacca acttcagcct
     3181 gctgaagcag gccggcgacg tggaggagaa ccccggcccc atgattgaac aagatggatt
     3241 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     3301 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     3361 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     3421 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     3481 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     3541 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     3601 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     3661 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     3721 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     3781 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     3841 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     3901 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     3961 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgattaag
     4021 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     4081 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     4141 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     4201 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     4261 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     4321 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     4381 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     4441 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     4501 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     4561 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac
     4621 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     4681 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     4741 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     4801 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     4861 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     4921 tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     4981 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     5041 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     5101 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     5161 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     5221 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     5281 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     5341 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     5401 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     5461 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     5521 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     5581 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     5641 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     5701 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     5761 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     5821 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     5881 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     5941 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     6001 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     6061 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     6121 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     6181 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     6241 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     6301 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     6361 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     6421 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     6481 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     6541 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     6601 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     6661 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     6721 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     6781 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     6841 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     6901 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     6961 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     7021 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7081 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7141 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7201 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7261 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7321 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7381 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7441 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7501 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7561 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7621 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     7681 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     7741 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     7801 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     7861 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     7921 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     7981 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     8041 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     8101 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     8161 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     8221 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     8281 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     8341 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     8401 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     8461 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     8521 tgtgactctg gtaactagag atc
//
