LOCUS       sequence-308691-        8043 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral CaTCH barcoding plasmid harboring a single barcode 
            cassette (BC1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8043)
  AUTHORS   Umkehrer C, Holstein F, Formenti L, Jude J, Froussios K, Neumann T, 
            Cronin SM, Haas L, Lipp JJ, Burkard TR, Fellner M, Wiesner T, Zuber 
            J, Obenauf AC
  TITLE     Isolating live cell clones from barcoded populations using 
            CRISPRa-inducible reporters.
  JOURNAL   Nat Biotechnol. 2020 Jul 27. pii: 10.1038/s41587-020-0614-0. doi: 
            10.1038/s41587-020-0614-0.
  PUBMED    32719478
REFERENCE   2  (bases 1 to 8043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Jul 27. pii: 10.1038/s41587-020-0614-0. doi: 
            10.1038/s41587-020-0614-0."
COMMENT     Sequence Label: CaTCH BC1
FEATURES             Location/Qualifiers
     source          1..8043
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             105..285
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    332..457
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    950..1183
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     primer_bind     1735..1759
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1838..1847
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1844..1846,1847..1849,1850..2563)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1844..1846,1847..1849,1850..2560)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    2605..2722
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2791..3002
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             join(3039..3041,3042..3044,3045..3758)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     misc_feature    3773..4361
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3826..3846)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4244..4255)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4433..4666
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4744..4865
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4781..4800)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4835..4854
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4905..5040
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5060..5079)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5061..5079)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5089..5106)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5089..5105)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5098..5120)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5247..5702
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5334..5353)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5544..5565
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5728..5832
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5833..5901,5902..6693)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6051..6070)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6864..7452
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7353..7372
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7606..7623
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7740..7761
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7776..7781,7782..7799,7800..7806)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7814..7830
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7819..7841
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7838..7854
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7838..7854
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7873..7893
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7875..7893
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        join(7921..8043,1..104)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
       61 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag
      121 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
      181 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
      241 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
      301 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
      361 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
      421 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
      481 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
      541 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
      601 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
      661 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
      721 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
      781 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
      841 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
      901 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
      961 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1021 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1081 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1141 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     1201 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     1261 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     1321 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgmwymmg
     1381 atctattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     1441 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     1501 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     1561 cccacctccc aaccccgagg ggaccctatc atatgctggg taccctgtct agagcgaacg
     1621 ggcgagcgcg cgcaaatggc ggcgactagt gatatccgtc tctcggcgct gtgctcgttc
     1681 cacacaccgg caggagaagc tttaggcgtg tacggtgggc gcctataaaa gcagagctcg
     1741 tttagtgaac cgtcagatcg cctggagcaa ttccacaaca cttttgtctt ataccaactt
     1801 tccgtaccac ttcctaccct cgtaaactgg aggatccgcc accatggtga gcaagggcga
     1861 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     1921 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     1981 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     2041 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     2101 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     2161 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     2221 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     2281 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     2341 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     2401 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     2461 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     2521 cgccgccggg atcactctcg gcatggacga gctgtacaag taagaattct agatcttgag
     2581 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2641 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2701 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2761 ccggctcgag tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     2821 agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa
     2881 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     2941 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     3001 aggtgtcgtg acgcggatcg taccccgaat ttatgctcat ggtgagcaag ggcgaggagc
     3061 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     3121 tcagcgtgag gggcgagggc gagggcgatg ccaccaacgg caagctgacc ctgaagttca
     3181 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgagccacg
     3241 gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     3301 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcacctaca
     3361 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     3421 gcgtcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aacttcaaca
     3481 gccacaacat ctatatcatg gccgtcaagc agaagaacgg catcaaggtg aacttcaaga
     3541 tccgccacaa cgtggaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     3601 ccatcggcga cggccccgtg ctgctgcccg acagccacta cctgagcacc cagtccgtgc
     3661 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc cgcaccgccg
     3721 ccgggatcac tctcggcatg gacgagctgt acaagtaacg cgttaagtcg acaatcaacc
     3781 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     3841 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     3901 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     3961 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     4021 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     4081 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     4141 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     4201 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     4261 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     4321 ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg
     4381 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     4441 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     4501 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     4561 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     4621 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt
     4681 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     4741 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     4801 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     4861 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     4921 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     4981 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     5041 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     5101 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     5161 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     5221 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     5281 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     5341 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     5401 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     5461 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     5521 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     5581 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     5641 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     5701 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5761 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5821 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     5881 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     5941 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     6001 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     6061 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6121 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6181 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6241 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6301 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6361 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6421 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6481 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6541 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6601 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6661 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6721 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6781 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6841 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6901 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6961 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     7021 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7081 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7141 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7201 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7261 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7321 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7381 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7441 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7501 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7561 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     7621 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     7681 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     7741 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     7801 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     7861 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     7921 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     7981 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     8041 gtg
//
