LOCUS       sequence-308707-       13463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  kdrl promoter driving EGFP fused to human KRAS4B-G12V with an 
            alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13463)
  AUTHORS   Fish JE, Flores-Suarez CP, Boudreau E, Herman AM, Gutierrez MC, 
            Gustafson D, DiStefano PV, Cui M, Chen Z, Berman De Ruiz K, 
            Schexnayder TS, Ward CS, Radovanovic I, Wythe JD
  TITLE     Somatic Gain of KRAS Function in the Endothelium is Sufficient to 
            Cause Vascular Malformations that Require MEK but not PI3K 
            Signaling.
  JOURNAL   Circ Res. 2020 Jun 17. doi: 10.1161/CIRCRESAHA.119.316500.
  PUBMED    32552404
REFERENCE   2  (bases 1 to 13463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Circ Res. 
            2020 Jun 17. doi: 10.1161/CIRCRESAHA.119.316500."
COMMENT     Sequence Label: JDW 770 (pTol2-kdrl:EGFP-hsKRAS4B-G12V-ac/Y)
FEATURES             Location/Qualifiers
     source          1..13463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(5302..5322)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(5342..5358)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    join(5731..5893,5894..5905)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     6254..6275
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6438..6542
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6543..6611,6612..7403)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6761..6780)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7574..8162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8063..8082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8316..8333
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8450..8471
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8486..8491,8492..8509,8510..8516)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8524..8540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8529..8551
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8548..8564
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8583..8603
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8585..8603
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     8640..8656
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8640..8656
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(8690..8701,8702..8889)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    9328..9462
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    9499..9706
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     9695..9712
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(9730..10443,10444..10446,10447..10449))
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     11021..11037
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11045..11065
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     polyA_signal    11128..11262
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    11264..11288
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(11290..11820,11821..11823,11824..11856))
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase K-Ras"
                     /label=K-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     CDS             complement(join(11881..12591,12592..12594,12595..12597))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(11881..12591,12592..12594,12595..12597))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      12594..12603
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(12606..12630)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca
       61 cacacacaca cacacactct ctcactctct ctcacacaca cacacacacg cacagtacta
      121 ttatctcttc tacaacaaat aaaaatattt gtaatgtatt tattgaagag ggttcattcc
      181 gctgtagcga acccttataa ataagggagt aagctaaagg gaaattaatg aatgaatatt
      241 tattaaaata tttaagatat tattttatct tgtttattat agacttaata tctgcacaac
      301 atcacttaaa ctagaatcac tttagcatag cctttctagc aaaaatcaga tttagtgtta
      361 gaaaatgaat gaatgaatca atatttgctt tttggcttag tccctttatt aatctggggt
      421 cgcaaaagtg gaatgaaccc gcaacttatc cttattatac tctaagaaaa aaaaagatga
      481 attatgcact acatcaaaat ataaagtaga ctaaaagtat tccatatcaa aacaaactgt
      541 aaaaaaaaaa caatattttt tattatatat atatttttta aatttaagat actgtacgca
      601 aaaatattgt acagtccaat aatctcttct tgatcacaaa aataattttt attgtatcac
      661 aaaaacttat ttttaatata tttattgagt aaactgtaac attaatagca tttggtacta
      721 tttaaaagtg tcttcttttg tcttgtttat tatagactta atatctggac aatataacta
      781 cgatcacttt agaatcacac ttctagcaag aatcaggctt attgtgttag tgtttaacta
      841 acagaaaatt aatatgaaat gtttcttagt aaatttttgc atcacaattt ttttaaatca
      901 aggtgcaacg ttgcattgtc tgattacata gcattcaaaa tacactggaa aaatatttac
      961 agtttttttt ttacttcaga aacaggatgt gcagctaatt ttatatcaga gtaaataatc
     1021 atggggcttc tttcaccttc cagcacccca tggtgtgtgg atgcaccaca gactgtttgt
     1081 ctttttgatg ctacaaacgt ggatatgctc cccgcaacgt acggcaatgc attgtccatt
     1141 catatccttt tatctacaca gtgctcagca tatatgagtg cacccctcac aaatcaatct
     1201 tttgaattaa tattttttaa gtgaagctat acaatattat atttgtgcat acacattatt
     1261 agtcagtgct gaagccaaat ctggagctta tctgacaaaa taacctacaa tgacggccca
     1321 aaaactagta caccacaatt tatatgttat agaaaaatat taaatacata tttaaaagag
     1381 gaaaaatcaa aagaagcaat tttttttcaa ttttagttga aattgttttg aatttaattg
     1441 cattaccttt caatgtctaa atatgtttgg tgactaaaat atttttattg taaaataatt
     1501 tattgtcctc tcctaacatt ccttccatca atatgtggat aaatcagatt gccattttct
     1561 tacccttaga aaaactgact taggacttaa gtcttatgat tagatatgat ttctggtata
     1621 tctgaaaccc tctatgagaa gtttggagaa tgtatcttga aagactattt ttattattat
     1681 tttgttcatt ttacccttca caaaagactt gaattactac ccgatagacg aaagtataat
     1741 aaatagttat gttattgtat atgatgacat gagacactta taccactgtt ctggcattgc
     1801 aaatctttaa ataaatgttt aaaataaata aataactaat ttaaatgtgg gcgaagcagt
     1861 ggcgcagtag gtagtgctgt tgcctcacaa caagaaggtt gctgggtcgc tggttcgatt
     1921 ctcggctcag ttggcgtttt tgtgtggagt ttgcatgttc tccctgcatt cgcgtgggtt
     1981 tactccgggt gctacggttt cccccacaat ccaaagacat gcggtacagg tgaattgggt
     2041 tggctaaatt gtccatagtg tctgagtgtg tgtgaatgag tgtttgtgga tgtttcccag
     2101 agatgggttg catctggaag ggcatccgct gcataaaaat gtgctggata agttggcggt
     2161 tcattccgct gtggcgaacc tagattaata aagggactaa gccaacaaga aaatgaatga
     2221 atgaataatt taaaggattt atttttatta ggtttttaaa taaataggtc ttacacttta
     2281 tattttctga tttttcatag tatatgtatt aattctgtta cttttaccat atcaaccatt
     2341 tggtagtggc tgatatttta gaaatagtgg gatgtgttga aaaaaatgct ttgaatttgt
     2401 caactaggaa tgcaatccaa agtaattgat ccctgatttt tttttcattg aagtatataa
     2461 ttttagatta cagatgttca ttggtattta atgttaacag atttaaatgc agatttaaat
     2521 gctgataaaa gccaccatgt aaagtgtatt atgcagtaaa ttgcaacatt tagtttttga
     2581 attagaaaga ttaagagata aattcttgta aaataaaagc tagtaatttt ttaccttttt
     2641 ctttatttcc aaagcattta cagacaatac atttcaggtt atgtcttcag gtttggcagc
     2701 agatgtttta cagagatatt tctcgaatac acaatattcg gcctagcatt gacctttcat
     2761 acccaagtct aactgtgaga tgaaatattt ttaaacctgg cacagatgta taaatacgcc
     2821 tgtttattat tattttaata ttatttatgt tgcagacttt aggaaatctt gtgtccatct
     2881 ctctgaccgc atgtcacaag gatcatgttt gtttttttcg ttctcctgtg caatcaagcc
     2941 ctctgaactg atcacatttc cctcagacca gtacggtggc tagataagac tcaggcagaa
     3001 acaattgctg gaagccgagc atgcagagga gtgggctttt aagacatctt atttttgaat
     3061 ctagtgccgt gaacacaata agagacttca caaaccattc tgtgtacgca gcttgagctc
     3121 tttccttcag ctcaacaatg acctcccctg ttaaaacaaa aagctcagcg ttatcagcac
     3181 atactttcta tggggtgaat gaggttttaa ctagcagaag gtgatgaata ttatgtttaa
     3241 acaatgctat tatactatgt aatgggccag acctttcaac acggaaattc tgaatatcaa
     3301 aagagcaggc ctagttctcc atttatctta aaatattatt tagagcgctt gtttctgagg
     3361 cttttatatt tcatttttat attctattac tatttataat aaaagtgcat ttaatatagt
     3421 ggaaattgtg cgctataatg taactaaata ttaaatttaa gtttatttgt acagagcttt
     3481 ttacagtaat tattgtttca atgcagcttt acaaaagatg cacatttttg cattacaatc
     3541 acataattgc attactttaa gtaaacctca gttacttaag ttacaatcaa atagcagtta
     3601 ttatatacag acacatgcaa ttttatagca tatgagtgat gtccaaatag caaaatgatt
     3661 gtagttgtta atattaatga ggctggatta ttaatcttgg cttcaccacg ccaaataaat
     3721 tgtgtgaacc tgaatgaaat attttaggga gacatgatga gtaacagcaa gttaaaaacc
     3781 gtgtgtgaga caccaccttg cggtcactca gcggtattac actccggctg aagcgcgtcc
     3841 tcgtgcgtgt tttggcgggg agcggtcgcg aggggcagcg ttggtttttt tccagtcaca
     3901 cagaaacttc atacaaaata caacttttta aaattacttc agatttttta gatacacaaa
     3961 atataatatg gagcagtttg ctgtcgaagt aaaaggtacg tttgcggtaa aagaaagata
     4021 tgtggtacca gtcaattcaa tgtataaaag atacgctatt gtgtaagtta tttactatga
     4081 gatgagctat tgtgtttttt tacaaaacaa ttatttatct tacatatttt aagtgatatt
     4141 aatataatga caaataacag tttcaactta ccttttgtaa gtaatttaac aggttatgtg
     4201 aagatatttc gtggtttgac gttcctttca cagacctgtc agagaaactc ctccacagac
     4261 agagccattc agacttggat gaagtgattg attccctctt caaggagatt ttaactctag
     4321 atgaagctgc aaaactacag gattcacagg tgtggaaaat acacttcttt tgaaatctac
     4381 ggaagccccc agggatcttg tagcgttgtg ttttgaagac atgacgtctt tattttgttc
     4441 tcgacataaa tatatattat ttcgtatttt aagttaacat cttacacaga aatcaggtaa
     4501 ttgtggttca atatttaata atttagttgt ttttgtagct ggaggaaatc gccatccagt
     4561 tgtggaactg ggctgtcacc aaacgtgttg gcacttcaat tacagaagag cagaaggcca
     4621 aaggtctgtc cttgtattag tcttttgttt tattaacttt aatataaatt agattttttt
     4681 taaataataa tacatgtatt aattaggcct aataattata attaataatg attttacttg
     4741 cctgtattaa gtatctattt aatactgttg ggggtttcaa tctgtggtct gggaaatgta
     4801 gaaatgttta ataaattaaa ataattggtc aatgtagtta atacaatttt aaacatgttt
     4861 aaattagcct tttatggacg cacaaatgaa catactagca ggagcggact taatcaataa
     4921 tcaaagttag cagccgccta gggccccagg gaattatggg tccctgacca atgccaagaa
     4981 gcctatatta atcatatatc tcgtttatac attttttaaa tcatatgttg taaaatctgt
     5041 gtatagggtc tgtttaaata aaataacatt gctcagtgaa aatgaacggc tgccgattca
     5101 caaacagcaa ttattcagtt aacttggatg atcgtgaatt aaatttgtta aaactttttt
     5161 ttttataatt tatatcaaaa tcttttcacc aaaatggctc atttagttta aatagttgta
     5221 aattagttgt ttttttttat ttgtgaatat attacattta attttaaata aaagaaaaag
     5281 ctggcgcgcc gaattcggag ccaacttttc tatacaaagt tgatagcttg gcgtaatcat
     5341 ggtcatagct gtttcctgct cgagggccca tctggcctgt gtttcagaca ccagggagtc
     5401 tctgctcacg tttcctgcta tttgcagcct ctctatcaag actaatacac ctcttcccgc
     5461 atcggctgcc tgtgagaggc ttttcagcac tgcaggattg cttttcagcc ccaaaagagc
     5521 taggcttgac actaacaatt ttgagaatca gcttctactg aagttaaatc tgaggtttta
     5581 caactttgag tagcgtgtac tggcattaga ttgtctgtct tatagtttga taattaaata
     5641 caaacagttc taaagcagga taaaaccttg tatgcatttc atttaatgtt ttttgagatt
     5701 aaaagcttaa acaagaatct ctagttttct ttcttgcttt tacttttact tccttaatac
     5761 tcaagtacaa ttttaatgga gtactttttt acttttactc aagtaagatt ctagccagat
     5821 acttttactt ttaattgagt aaaattttcc ctaagtactt gtactttcac ttgagtaaaa
     5881 tttttgagta ctttttacac ctctgtcaag aactcctgga caaacctctg acctgtgtgg
     5941 aacagagtgg atatgggtgt ctgaacagat attcacgtct tttgcagatc agagggcatt
     6001 tctggtgttg tgcaatgacc tgggtccaac aggtaggcgt actcgtatcc aagagcattg
     6061 ttggacagga ggtagtctcc attcctataa agaggaagga atggcaccat gtagtagccg
     6121 tcattgtggc caattggtgc attggtacgt gggtagatgg accggggagg ctgatgagtt
     6181 cttagccagc gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     6241 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6301 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6361 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     6421 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6481 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6541 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6601 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     6661 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     6721 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     6781 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     6841 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     6901 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     6961 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     7021 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7081 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     7141 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7201 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7261 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7321 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7381 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7441 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7501 tgaccaaaat gccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7561 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7621 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7681 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     7741 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7801 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7861 agttaccaga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7921 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     7981 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8041 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8101 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8161 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8221 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8281 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8341 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     8401 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     8461 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     8521 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     8581 gcgcaattaa ccctcactaa agggaacaaa agctggagct ccaccgcggt ggcggccgct
     8641 ctagaactag tggatctgct gggcttgctg aaggtagggg gtcaagaacc agaggtgtaa
     8701 agtacttgag taattttact tgattactgt acttaagtat tatttttggg gatttttact
     8761 ttacttgagt acaattaaaa atcaatactt ttacttttac ttaattacat ttttttagaa
     8821 aaaaaagtac tttttactcc ttacaatttt atttacagtc aaaaagtact tattttttgg
     8881 agatcacttc attctatttt cccttgctat taccaaacca attgaattgc gctgatgccc
     8941 agtttaattt aaatgttatt tattctgcct atgaaaatcg ttttcacatt atatgaaatt
     9001 ggtcagacat gttcattggt cctttggaag tgacgtcatg tcacatctat taccacaatg
     9061 cacagcacct tgacctggaa attagggaaa ttataacagt caatcagtgg aagaaaatgg
     9121 aggaagtatg tgattcatca gcagctgcga gcagcacagt ccaaaatcag ccacaggatc
     9181 aagagcaccc gtggccgtat cttcgcagat ctgatctaga ggatcataat cagccatacc
     9241 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     9301 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     9361 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     9421 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcatcatcga tggtacccac
     9481 cgcggtggcg gcgatctctc cccagcatgc ctgctattgt cttcccaatc ctcccccttg
     9541 ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca
     9601 atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc
     9661 acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga ggctgatcag
     9721 cgggccgctt tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
     9781 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactggta
     9841 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
     9901 gtagtggtcg gcgagctgca cgccgccgtc ctcgatgttg tggcggatct tgaagttggc
     9961 cttgatgccg ttcttctgct tgtcggcggt gatatagacg ttgtggctgt tgtagttgta
    10021 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
    10081 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
    10141 gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa
    10201 gtcgtgctgc ttcatgtggt cggggtagcg ggcgaagcac tgcaggccgt agcccagggt
    10261 ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatca gcttcagggt
    10321 cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt
    10381 tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt
    10441 gctcaccatg ttgtcagacc tggtaactcc ttactgtaac ttattgcacc tgatgaacaa
    10501 aagatgtgaa ctgaacgcaa gtaggaggct aatagaggct agtacagtac aaagatcccc
    10561 aaatgatgtg tgccggcctt agtggaggca gacccctggt tatatacctg tgctgcagaa
    10621 agaggggctt acaaatggat ctctctggaa tggcattatc tcatggtggg aacagtgttg
    10681 tcagcagaat cagcggggtg gactctgacc tgagaatgac agtctagcac tgaatggctc
    10741 agacaatact cttgtggcca actatcaccg gaaaataagt gcctgccaaa acaaaccaga
    10801 ccagatgaag ctacatggct gtagaacgtc agtatttaat gtgttgtggc cacttggttc
    10861 accagattta agagctaatt tttttaaata tcacagataa tcaatcgctt tttttttttc
    10921 ttgactgaaa agcttagact ggaaaattct ttgaaaaagt ttgcactaca gaaaataaat
    10981 acataacatc ctgcactgaa tgcacactat taatggtacc gtaaaacgac ggccagtgaa
    11041 ttatcaacta tgtataataa agttggaaaa aacctcccac acctccccct gaacctgaaa
    11101 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
    11161 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    11221 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcccccactt tgtacaagaa
    11281 agctgggtct tacataatta cacactttgt ctttgacttc tttttcttct ttttaccatc
    11341 tttgctcatc ttttctttat gttttcgaat ttctcgaact aatgtataga aggcatcatc
    11401 aacaccctgt cttgtctttg ctgatgtttc aataaaagga attccataac ttcttgctaa
    11461 gtcctgagcc tgttttgtgt ctactgttct ggaaggcaaa tcacatttat ttcctactag
    11521 gaccataggt acatcttcag agtccttaac tcttttaatt tgttctctat aatggtgaat
    11581 atcttcaaat gatttagtat tatttatggc aaatacacaa agaaagccct ccccagtcct
    11641 catgtactgg tccctcattg cactgtactc ctcttgacct gctgtgtcga gaatatccaa
    11701 gagacaggtt tctccatcaa ttactacttg cttcctgtag gaatcctcta ttgttggatc
    11761 atattcgtcc acaaaatgat tctgaattag ctgtatcgtc aaggcactct tgcctacgcc
    11821 aacagctcca actaccacaa gtttatattc agtcataagc ttgagctcga gatctgagta
    11881 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
    11941 catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg
    12001 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
    12061 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt
    12121 cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg
    12181 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag
    12241 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa
    12301 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
    12361 catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt
    12421 gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta
    12481 ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc
    12541 cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatggt
    12601 ggcttagcct gcttttttgt acaaacttgg gtcgaattcg gcttctacac tatgtagtga
    12661 aggtgtaaca gccatttatc tatcttcaca ccatcccact atacctctga cttttctact
    12721 gggcttttcc ctctcgaagg ctttatatga gtgaagcgag gacggaagga cgcaacgtca
    12781 ccatagtaac cctgcagcga tactcgtaaa gctacgtgta ctgatttaac taaatcgata
    12841 ggctatctac ttattcaggc taaatgactt gcatagagtt attaggaaca taccaacaca
    12901 agaccaacag aaacaagaaa tacatcataa catttttata cccatcaaag taaaaaacat
    12961 gaaatatgca ctatagtaga ctgaaaatac agaactctgg tcacacttta taataaggtt
    13021 ttattgttga ctgtatttac taacataaac aaaaccaata cagcatttac taatcagttc
    13081 aacatttact aatgcataag acacaaattc ctctttttta acattactta atacagagtt
    13141 aattaactaa gaatgaacaa ctgtattttc actaactagc ataaacaaac aattaaatgt
    13201 attgttcatg gtctgttcgt gttagtgaat gcattactaa tacaacttta ttgtaaagtt
    13261 ttacttgaac tgctattaaa aagtatggcc tactataata agaatatgat cgatttaata
    13321 agaatttact ggaaatttga tttaaaaaga gttaatacgt atcatacatc acatacatca
    13381 tgggttgatt tttttaagtt acataactca ttccaagtgt ttttaaatta ctgcacatat
    13441 tatctctctc tctctctctt tct
//
