LOCUS       sequence-308710-       13449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  kdrl promoter driving EYFP fused to human KRAS4B with an 
            alpha-crystallin zsYellow reporter flanked by Tol2 sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13449)
  AUTHORS   Fish JE, Flores-Suarez CP, Boudreau E, Herman AM, Gutierrez MC, 
            Gustafson D, DiStefano PV, Cui M, Chen Z, Berman De Ruiz K, 
            Schexnayder TS, Ward CS, Radovanovic I, Wythe JD
  TITLE     Somatic Gain of KRAS Function in the Endothelium is Sufficient to 
            Cause Vascular Malformations that Require MEK but not PI3K 
            Signaling.
  JOURNAL   Circ Res. 2020 Jun 17. doi: 10.1161/CIRCRESAHA.119.316500.
  PUBMED    32552404
REFERENCE   2  (bases 1 to 13449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Circ Res. 
            2020 Jun 17. doi: 10.1161/CIRCRESAHA.119.316500."
COMMENT     Sequence Label: JDW 771 (pTol2-kdrl:EYFP-hsKRAS4B-WT-ac/Y)
FEATURES             Location/Qualifiers
     source          1..13449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    901..925
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      928..937
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(934..936,937..939,940..1650)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1669..2235
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=K-Ras
                     /translation="MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     protein_bind    complement(2237..2261)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    2263..2397
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(2460..2480)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2488..2504)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             join(3076..3078,3079..3081,3082..3795)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3813..3830)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3819..4026
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    4063..4197
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(4636..4823,4824..4835)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(4869..4885)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4869..4885)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4922..4942)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4922..4940)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4961..4977)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4974..4996)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4985..5001
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5009..5015,5016..5033,5034..5039))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5054..5075
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5192..5209)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5363..5951)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5443..5462)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6122..6913,6914..6982))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6745..6764
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6983..7087)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7250..7271)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(7620..7631,7632..7794)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     8167..8183
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8203..8223
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
       61 gtgtgtgaga aagagagaga gagagagata atatgtgcag taatttaaaa acacttggaa
      121 tgagttatgt aacttaaaaa aatcaaccca tgatgtatgt gatgtatgat acgtattaac
      181 tctttttaaa tcaaatttcc agtaaattct tattaaatcg atcatattct tattatagta
      241 ggccatactt tttaatagca gttcaagtaa aactttacaa taaagttgta ttagtaatgc
      301 attcactaac acgaacagac catgaacaat acatttaatt gtttgtttat gctagttagt
      361 gaaaatacag ttgttcattc ttagttaatt aactctgtat taagtaatgt taaaaaagag
      421 gaatttgtgt cttatgcatt agtaaatgtt gaactgatta gtaaatgctg tattggtttt
      481 gtttatgtta gtaaatacag tcaacaataa aaccttatta taaagtgtga ccagagttct
      541 gtattttcag tctactatag tgcatatttc atgtttttta ctttgatggg tataaaaatg
      601 ttatgatgta tttcttgttt ctgttggtct tgtgttggta tgttcctaat aactctatgc
      661 aagtcattta gcctgaataa gtagatagcc tatcgattta gttaaatcag tacacgtagc
      721 tttacgagta tcgctgcagg gttactatgg tgacgttgcg tccttccgtc ctcgcttcac
      781 tcatataaag ccttcgagag ggaaaagccc agtagaaaag tcagaggtat agtgggatgg
      841 tgtgaagata gataaatggc tgttacacct tcactacata gtgtagaagc cgaattcgac
      901 ccaagtttgt acaaaaaagc aggctaagcc accatggtga gcaagggcga ggagctgttc
      961 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     1021 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     1081 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg
     1141 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     1201 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1261 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1321 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     1381 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     1441 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     1501 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc
     1561 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1621 atcactctcg gcatggacga gctgtacaag tccggactca gatccaccat gactgaatat
     1681 aaacttgtgg tagttggagc tggtggcgta ggcaagagtg ccttgacgat acagctaatt
     1741 cagaatcatt ttgtggacga atatgatcca acaatagagg attcctacag gaagcaagta
     1801 gtaattgatg gagaaacctg tctcttggat attctcgaca cagcaggtca agaggagtac
     1861 agtgcaatga gggaccagta catgaggact ggggagggct ttctttgtgt atttgccata
     1921 aataatacta aatcatttga agatattcac cattatagag aacaaattaa aagagttaag
     1981 gactctgaag atgtacctat ggtcctagta ggaaataaat gtgatttgcc ttccagaaca
     2041 gtagacacaa aacaggctca ggacttagca agaagttatg gaattccttt tattgaaaca
     2101 tcagcaaaga caagacaggg tgttgatgat gccttctata cattagttcg agaaattcga
     2161 aaacataaag aaaagatgag caaagatggt aaaaagaaga aaaagaagtc aaagacaaag
     2221 tgtgtaatta tgtaagaccc agctttcttg tacaaagtgg gggatccaga catgataaga
     2281 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     2341 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     2401 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttcc
     2461 aactttatta tacatagttg ataattcact ggccgtcgtt ttacggtacc attaatagtg
     2521 tgcattcagt gcaggatgtt atgtatttat tttctgtagt gcaaactttt tcaaagaatt
     2581 ttccagtcta agcttttcag tcaagaaaaa aaaaaagcga ttgattatct gtgatattta
     2641 aaaaaattag ctcttaaatc tggtgaacca agtggccaca acacattaaa tactgacgtt
     2701 ctacagccat gtagcttcat ctggtctggt ttgttttggc aggcacttat tttccggtga
     2761 tagttggcca caagagtatt gtctgagcca ttcagtgcta gactgtcatt ctcaggtcag
     2821 agtccacccc gctgattctg ctgacaacac tgttcccacc atgagataat gccattccag
     2881 agagatccat ttgtaagccc ctctttctgc agcacaggta tataaccagg ggtctgcctc
     2941 cactaaggcc ggcacacatc atttggggat ctttgtactg tactagcctc tattagcctc
     3001 ctacttgcgt tcagttcaca tcttttgttc atcaggtgca ataagttaca gtaaggagtt
     3061 accaggtctg acaacatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     3121 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     3181 ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc
     3241 gtgccctggc ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt cgcccgctac
     3301 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     3361 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     3421 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     3481 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc
     3541 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc
     3601 ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     3661 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
     3721 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     3781 gagctgtaca agtaaagcgg cccgctgatc agcctcgact gtgccttcta gttgccagcc
     3841 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     3901 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     3961 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     4021 tggggagaga tcgccgccac cgcggtgggt accatcgatg atgatccaga catgataaga
     4081 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     4141 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     4201 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     4261 agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctaga tcagatctgc
     4321 gaagatacgg ccacgggtgc tcttgatcct gtggctgatt ttggactgtg ctgctcgcag
     4381 ctgctgatga atcacatact tcctccattt tcttccactg attgactgtt ataatttccc
     4441 taatttccag gtcaaggtgc tgtgcattgt ggtaatagat gtgacatgac gtcacttcca
     4501 aaggaccaat gaacatgtct gaccaatttc atataatgtg aaaacgattt tcataggcag
     4561 aataaataac atttaaatta aactgggcat cagcgcaatt caattggttt ggtaatagca
     4621 agggaaaata gaatgaagtg atctccaaaa aataagtact ttttgactgt aaataaaatt
     4681 gtaaggagta aaaagtactt ttttttctaa aaaaatgtaa ttaagtaaaa gtaaaagtat
     4741 tgatttttaa ttgtactcaa gtaaagtaaa aatccccaaa aataatactt aagtacagta
     4801 atcaagtaaa attactcaag tactttacac ctctggttct tgacccccta ccttcagcaa
     4861 gcccagcaga tccactagtt ctagagcggc cgccaccgcg gtggagctcc agcttttgtt
     4921 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     4981 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     5041 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     5101 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     5161 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     5221 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     5281 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     5341 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     5401 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     5461 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     5521 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     5581 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     5641 accgctgcgc cttatctggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     5701 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     5761 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     5821 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     5881 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     5941 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6001 actcacgtta aggcattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6061 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6121 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     6181 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     6241 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     6301 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     6361 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     6421 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6481 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     6541 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     6601 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     6661 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     6721 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     6781 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     6841 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     6901 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     6961 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     7021 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7081 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
     7141 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     7201 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     7261 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     7321 gggcgatggc ccacgctggc taagaactca tcagcctccc cggtccatct acccacgtac
     7381 caatgcacca attggccaca atgacggcta ctacatggtg ccattccttc ctctttatag
     7441 gaatggagac tacctcctgt ccaacaatgc tcttggatac gagtacgcct acctgttgga
     7501 cccaggtcat tgcacaacac cagaaatgcc ctctgatctg caaaagacgt gaatatctgt
     7561 tcagacaccc atatccactc tgttccacac aggtcagagg tttgtccagg agttcttgac
     7621 agaggtgtaa aaagtactca aaaattttac tcaagtgaaa gtacaagtac ttagggaaaa
     7681 ttttactcaa ttaaaagtaa aagtatctgg ctagaatctt acttgagtaa aagtaaaaaa
     7741 gtactccatt aaaattgtac ttgagtatta aggaagtaaa agtaaaagca agaaagaaaa
     7801 ctagagattc ttgtttaagc ttttaatctc aaaaaacatt aaatgaaatg catacaaggt
     7861 tttatcctgc tttagaactg tttgtattta attatcaaac tataagacag acaatctaat
     7921 gccagtacac gctactcaaa gttgtaaaac ctcagattta acttcagtag aagctgattc
     7981 tcaaaattgt tagtgtcaag cctagctctt ttggggctga aaagcaatcc tgcagtgctg
     8041 aaaagcctct cacaggcagc cgatgcggga agaggtgtat tagtcttgat agagaggctg
     8101 caaatagcag gaaacgtgag cagagactcc ctggtgtctg aaacacaggc cagatgggcc
     8161 ctcgagcagg aaacagctat gaccatgatt acgccaagct atcaactttg tatagaaaag
     8221 ttggctccga attcggcgcg ccagcttttt cttttattta aaattaaatg taatatattc
     8281 acaaataaaa aaaaacaact aatttacaac tatttaaact aaatgagcca ttttggtgaa
     8341 aagattttga tataaattat aaaaaaaaaa gttttaacaa atttaattca cgatcatcca
     8401 agttaactga ataattgctg tttgtgaatc ggcagccgtt cattttcact gagcaatgtt
     8461 attttattta aacagaccct atacacagat tttacaacat atgatttaaa aaatgtataa
     8521 acgagatata tgattaatat aggcttcttg gcattggtca gggacccata attccctggg
     8581 gccctaggcg gctgctaact ttgattattg attaagtccg ctcctgctag tatgttcatt
     8641 tgtgcgtcca taaaaggcta atttaaacat gtttaaaatt gtattaacta cattgaccaa
     8701 ttattttaat ttattaaaca tttctacatt tcccagacca cagattgaaa cccccaacag
     8761 tattaaatag atacttaata caggcaagta aaatcattat taattataat tattaggcct
     8821 aattaataca tgtattatta tttaaaaaaa atctaattta tattaaagtt aataaaacaa
     8881 aagactaata caaggacaga cctttggcct tctgctcttc tgtaattgaa gtgccaacac
     8941 gtttggtgac agcccagttc cacaactgga tggcgatttc ctccagctac aaaaacaact
     9001 aaattattaa atattgaacc acaattacct gatttctgtg taagatgtta acttaaaata
     9061 cgaaataata tatatttatg tcgagaacaa aataaagacg tcatgtcttc aaaacacaac
     9121 gctacaagat ccctgggggc ttccgtagat ttcaaaagaa gtgtattttc cacacctgtg
     9181 aatcctgtag ttttgcagct tcatctagag ttaaaatctc cttgaagagg gaatcaatca
     9241 cttcatccaa gtctgaatgg ctctgtctgt ggaggagttt ctctgacagg tctgtgaaag
     9301 gaacgtcaaa ccacgaaata tcttcacata acctgttaaa ttacttacaa aaggtaagtt
     9361 gaaactgtta tttgtcatta tattaatatc acttaaaata tgtaagataa ataattgttt
     9421 tgtaaaaaaa cacaatagct catctcatag taaataactt acacaatagc gtatctttta
     9481 tacattgaat tgactggtac cacatatctt tcttttaccg caaacgtacc ttttacttcg
     9541 acagcaaact gctccatatt atattttgtg tatctaaaaa atctgaagta attttaaaaa
     9601 gttgtatttt gtatgaagtt tctgtgtgac tggaaaaaaa ccaacgctgc ccctcgcgac
     9661 cgctccccgc caaaacacgc acgaggacgc gcttcagccg gagtgtaata ccgctgagtg
     9721 accgcaaggt ggtgtctcac acacggtttt taacttgctg ttactcatca tgtctcccta
     9781 aaatatttca ttcaggttca cacaatttat ttggcgtggt gaagccaaga ttaataatcc
     9841 agcctcatta atattaacaa ctacaatcat tttgctattt ggacatcact catatgctat
     9901 aaaattgcat gtgtctgtat ataataactg ctatttgatt gtaacttaag taactgaggt
     9961 ttacttaaag taatgcaatt atgtgattgt aatgcaaaaa tgtgcatctt ttgtaaagct
    10021 gcattgaaac aataattact gtaaaaagct ctgtacaaat aaacttaaat ttaatattta
    10081 gttacattat agcgcacaat ttccactata ttaaatgcac ttttattata aatagtaata
    10141 gaatataaaa atgaaatata aaagcctcag aaacaagcgc tctaaataat attttaagat
    10201 aaatggagaa ctaggcctgc tcttttgata ttcagaattt ccgtgttgaa aggtctggcc
    10261 cattacatag tataatagca ttgtttaaac ataatattca tcaccttctg ctagttaaaa
    10321 cctcattcac cccatagaaa gtatgtgctg ataacgctga gctttttgtt ttaacagggg
    10381 aggtcattgt tgagctgaag gaaagagctc aagctgcgta cacagaatgg tttgtgaagt
    10441 ctcttattgt gttcacggca ctagattcaa aaataagatg tcttaaaagc ccactcctct
    10501 gcatgctcgg cttccagcaa ttgtttctgc ctgagtctta tctagccacc gtactggtct
    10561 gagggaaatg tgatcagttc agagggcttg attgcacagg agaacgaaaa aaacaaacat
    10621 gatccttgtg acatgcggtc agagagatgg acacaagatt tcctaaagtc tgcaacataa
    10681 ataatattaa aataataata aacaggcgta tttatacatc tgtgccaggt ttaaaaatat
    10741 ttcatctcac agttagactt gggtatgaaa ggtcaatgct aggccgaata ttgtgtattc
    10801 gagaaatatc tctgtaaaac atctgctgcc aaacctgaag acataacctg aaatgtattg
    10861 tctgtaaatg ctttggaaat aaagaaaaag gtaaaaaatt actagctttt attttacaag
    10921 aatttatctc ttaatctttc taattcaaaa actaaatgtt gcaatttact gcataataca
    10981 ctttacatgg tggcttttat cagcatttaa atctgcattt aaatctgtta acattaaata
    11041 ccaatgaaca tctgtaatct aaaattatat acttcaatga aaaaaaaatc agggatcaat
    11101 tactttggat tgcattccta gttgacaaat tcaaagcatt tttttcaaca catcccacta
    11161 tttctaaaat atcagccact accaaatggt tgatatggta aaagtaacag aattaataca
    11221 tatactatga aaaatcagaa aatataaagt gtaagaccta tttatttaaa aacctaataa
    11281 aaataaatcc tttaaattat tcattcattc attttcttgt tggcttagtc cctttattaa
    11341 tctaggttcg ccacagcgga atgaaccgcc aacttatcca gcacattttt atgcagcgga
    11401 tgcccttcca gatgcaaccc atctctggga aacatccaca aacactcatt cacacacact
    11461 cagacactat ggacaattta gccaacccaa ttcacctgta ccgcatgtct ttggattgtg
    11521 ggggaaaccg tagcacccgg agtaaaccca cgcgaatgca gggagaacat gcaaactcca
    11581 cacaaaaacg ccaactgagc cgagaatcga accagcgacc cagcaacctt cttgttgtga
    11641 ggcaacagca ctacctactg cgccactgct tcgcccacat ttaaattagt tatttattta
    11701 ttttaaacat ttatttaaag atttgcaatg ccagaacagt ggtataagtg tctcatgtca
    11761 tcatatacaa taacataact atttattata ctttcgtcta tcgggtagta attcaagtct
    11821 tttgtgaagg gtaaaatgaa caaaataata ataaaaatag tctttcaaga tacattctcc
    11881 aaacttctca tagagggttt cagatatacc agaaatcata tctaatcata agacttaagt
    11941 cctaagtcag tttttctaag ggtaagaaaa tggcaatctg atttatccac atattgatgg
    12001 aaggaatgtt aggagaggac aataaattat tttacaataa aaatatttta gtcaccaaac
    12061 atatttagac attgaaaggt aatgcaatta aattcaaaac aatttcaact aaaattgaaa
    12121 aaaaattgct tcttttgatt tttcctcttt taaatatgta tttaatattt ttctataaca
    12181 tataaattgt ggtgtactag tttttgggcc gtcattgtag gttattttgt cagataagct
    12241 ccagatttgg cttcagcact gactaataat gtgtatgcac aaatataata ttgtatagct
    12301 tcacttaaaa aatattaatt caaaagattg atttgtgagg ggtgcactca tatatgctga
    12361 gcactgtgta gataaaagga tatgaatgga caatgcattg ccgtacgttg cggggagcat
    12421 atccacgttt gtagcatcaa aaagacaaac agtctgtggt gcatccacac accatggggt
    12481 gctggaaggt gaaagaagcc ccatgattat ttactctgat ataaaattag ctgcacatcc
    12541 tgtttctgaa gtaaaaaaaa aactgtaaat atttttccag tgtattttga atgctatgta
    12601 atcagacaat gcaacgttgc accttgattt aaaaaaattg tgatgcaaaa atttactaag
    12661 aaacatttca tattaatttt ctgttagtta aacactaaca caataagcct gattcttgct
    12721 agaagtgtga ttctaaagtg atcgtagtta tattgtccag atattaagtc tataataaac
    12781 aagacaaaag aagacacttt taaatagtac caaatgctat taatgttaca gtttactcaa
    12841 taaatatatt aaaaataagt ttttgtgata caataaaaat tatttttgtg atcaagaaga
    12901 gattattgga ctgtacaata tttttgcgta cagtatctta aatttaaaaa atatatatat
    12961 aataaaaaat attgtttttt ttttacagtt tgttttgata tggaatactt ttagtctact
    13021 ttatattttg atgtagtgca taattcatct ttttttttct tagagtataa taaggataag
    13081 ttgcgggttc attccacttt tgcgacccca gattaataaa gggactaagc caaaaagcaa
    13141 atattgattc attcattcat tttctaacac taaatctgat ttttgctaga aaggctatgc
    13201 taaagtgatt ctagtttaag tgatgttgtg cagatattaa gtctataata aacaagataa
    13261 aataatatct taaatatttt aataaatatt cattcattaa tttcccttta gcttactccc
    13321 ttatttataa gggttcgcta cagcggaatg aaccctcttc aataaataca ttacaaatat
    13381 ttttatttgt tgtagaagag ataatagtac tgtgcgtgtg tgtgtgtgtg tgagagagag
    13441 tgagagagt
//
