LOCUS       sequence-308737-       12912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Piezo1-based fluorescent force sensor 
            GenEPi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12912)
  AUTHORS   Yaganoglu S, Kalyviotis K, Vagena-Pantoula C, Julich D, Gaub BM, 
            Welling M, Lopes T, Lachowski D, Tang SS, Hernandez ADR, Salem V, 
            Muller DJ, Holley SA, Vermot J, Shi J, Helassa N, Torok K, Pantazis 
            P
  TITLE     Highly specific and non-invasive imaging of Piezo1-dependent 
            activity across scales using GenEPi.
  JOURNAL   Nat Commun. 2023 Jul 19;14(1):4352. doi: 10.1038/s41467-023-40134-y.
  PUBMED    37468521
REFERENCE   2  (bases 1 to 12912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-07-19"; volume: "14(1):4352. doi"; pages: " 
            10.1038/s41467-023-40134-y"
COMMENT     Sequence Label: pCMV-GenEPi
FEATURES             Location/Qualifiers
     source          1..12912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             66..860
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             66..860
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(120..139)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     730..749
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1048..1067)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1092..1139
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1468..2056
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1957..1976
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2231..2534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2535..2738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2689..2709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(10384..10392,10393..10410,10411..10413,10414..10446,
                     10447..10449,10450..10473,10474..10500,10501..10557,
                     10558..10563,10564..10833,10834..10851,10852..11286,
                     11287..11292,11293..11736)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKYNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREAAIDGDGQVNYEEFVQMMTAK"
     regulatory      10480..10489
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     10770..10791
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(10897..10918)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(11158..11177)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    11870..11991
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11907..11926)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11961..11980
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(11998..12453)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12135..12156)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12347..12366
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        12480..12584
                     /gene="bla"
                     /label=AmpR promoter
     promoter        join(12586..12912,1..31)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        12586..12777
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(12609..12629)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      join(12794..12912,1..17)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt
       61 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
      121 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
      181 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
      241 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
      301 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
      361 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
      421 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
      481 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
      541 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
      601 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
      661 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
      721 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
      781 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
      841 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
      901 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
      961 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     1021 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     1081 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca
     1141 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     1201 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     1261 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata
     1321 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1381 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1441 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1501 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1561 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1621 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1681 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1741 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1801 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1861 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1921 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1981 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2041 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2101 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2161 cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     2221 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2401 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2461 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2521 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2581 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2641 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2701 gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc
     2761 gctagcgcta ccggactcag atctcgagct caagcttatc gcatggagcc gcacgtgctc
     2821 ggcgcggtcc tgtactggct gctgctgccc tgcgcgctgc tggctgcctg cctgctccgc
     2881 ttcagcggac tctcgctggt ctacctgctc ttcctgctgc tgctgccctg gttccccggc
     2941 cccacccgat gcggcctcca aggtcacaca ggccgcctcc tgcgggcatt gctgggcctc
     3001 agcctgctct tcctggtggc ccatctcgcc ctccagatct gcctgcatac tgtgccccgc
     3061 ctggaccagc tcctgggacc cagctgcagc cgctgggaga ccctctcgcg acacataggg
     3121 gtcacaaggc tggacctgaa ggacatcccc aacgccatcc ggctggtggc ccctgacctg
     3181 ggcatcttgg tggtctcctc tgtctgcctc ggcatctgcg ggcgccttgc aaggaacacc
     3241 cggcagagcc cacatccacg ggagctggat gatgatgaga gggatgtgga tgccagcccg
     3301 acggcagggc tgcaggaagc agcaacgctg gcccctacac ggaggtcacg gctggccgct
     3361 cgtttccgag tcacggccca ctggctgctg gtggcggctg ggcgggtcct ggccgtaaca
     3421 ctgcttgcac tggcaggcat cgcccacccc tcggccctct ccagtgtcta cctgctgctc
     3481 ttcctggccc tctgcacctg gtgggcctgc cactttccca tcagcactcg gggcttcagc
     3541 agactctgcg ccgcggtggg gtgcttcggc gccggccatc tcatctgcct ctactgctac
     3601 cagatgccct tggcacaggc tctgctcccg cctgccggca tctgggctag ggtgctgggt
     3661 ctcaaggact tcgtgggtcc caccaactgc tccagccccc acgcgctggt cctcaacacc
     3721 ggcctggact ggcctgtgta tgccagcccc ggcgtcctcc tgctgctgtg ctacgccacg
     3781 gcctctctgc gcaagctccg cgcgtaccgc ccctccggcc agaggaagga ggcggcaaag
     3841 gggtatgagg ctcgggagct ggagctagca gagctggacc agtggcccca ggaacgggag
     3901 tctgaccagc acgtggtgcc cacagcaccc gacaccgagg ctgataactg catcgtgcac
     3961 gagctgaccg gccagagctc cctcctgcgg cggcctgtgc ggcccaagcg ggctgagccc
     4021 ggggaggcgt ctccgctcca cagcctgggc cacctcatca tggaccagag ctatgtgtgc
     4081 gcgctcattg ccatgatggt atggagcatc acctaccaca gctggctgac cttcgtactg
     4141 ctgctctggg cctgcctcat ctggacggtg cgcagccgcc accaactggc catgctgtgc
     4201 tcgccctgca tcctgctgta tgggatgacg ctgtgctgcc tacgctacgt gtgggccatg
     4261 gacctgcgcc ctgagctgcc caccaccctg ggccccgtca gcctgcgcca gctggggctg
     4321 gagcacaccc gctacccctg tctggacctt ggtgccatgt tgctctacac cctgaccttc
     4381 tggctcctgc tgcgccagtt tgtgaaagag aagctgctga agtgggcaga gtctccagct
     4441 gcgctgacgg aggtcaccgt ggcagacaca gagcccacgc ggacgcagac gctgttgcag
     4501 agcctggggg agctggtgaa gggcgtgtac gccaagtact ggatctatgt gtgtgctggc
     4561 atgttcatcg tggtcagctt cgccggccgc ctcgtggtct acaagattgt ctacatgttc
     4621 ctcttcctgc tctgcctcac cctcttccag gtctactaca gcctgtggcg gaagctgctc
     4681 aaggccttct ggtggctcgt ggtggcctac accatgctgg tcctcatcgc cgtctacacc
     4741 ttccagttcc aggacttccc tgcctactgg cgcaacctca ctggcttcac cgacgagcag
     4801 ctgggggacc tgggcctgga gcagttcagc gtgtccgagc tcttctccag catcctggtg
     4861 cccggcttct tcctcctggc ctgcatcctg cagctgcact acttccacag gcccttcatg
     4921 cagctcaccg acatggagca cgtgtccctg cctggcacgc gcctcccgcg ctgggctcac
     4981 aggcaggatg cagtgagtgg gaccccactg ctgcgggagg agcagcagga gcatcagcag
     5041 cagcagcagg aggaggagga ggaggaggag gactccaggg acgaggggct gggcgtggcc
     5101 actccccacc aggccacgca ggtgcctgaa ggggcagcca agtggggcct ggtggctgag
     5161 cggctgctgg agctggcagc cggcttctcg gacgtcctct cacgcgtgca ggtgttcctg
     5221 cggcggctgc tggagcttca cgttttcaag ctggtggccc tgtacaccgt ctgggtggcc
     5281 ctgaaggagg tgtcggtgat gaacctgctg ctggtggtgc tgtgggcctt cgccctgccc
     5341 tacccacgct tccggcccat ggcctcctgc ctgtccaccg tgtggacctg cgtcatcatc
     5401 gtgtgtaaga tgctgtacca gctcaaggtt gtcaaccccc aggagtattc cagcaactgc
     5461 accgagccct tccccaacag caccaacttg ctgcccacgg agatcagcca gtccctgctg
     5521 taccgggggc ccgtggaccc tgccaactgg tttggggtgc ggaaagggtt ccccaacctg
     5581 ggctacatcc agaaccacct gcaagtgctg ctgctgctgg tattcgaggc catcgtgtac
     5641 cggcgccagg agcactaccg ccggcagcac cagctggccc cgctgcctgc ccaggccgtg
     5701 tttgccagcg gcacccgcca gcagctggac caggatctgc tcggctgcct caagtacttc
     5761 atcaacttct tcttctacaa attcgggctg gagatctgct tcctgatggc cgtgaacgtg
     5821 atcgggcagc gcatgaactt tctggtgacc ctgcacggtt gctggctggt ggccatcctc
     5881 acccgcaggc accgccaggc cattgcccgc ctctggccca actactgcct cttcctggcg
     5941 ctgttcctgc tgtaccagta cctgctgtgc ctggggatgc ccccggccct gtgcattgat
     6001 tatccctggc gctggagccg ggccgtcccc atgaactccg cactcatcaa gtggctgtac
     6061 ctgcctgatt tcttccgggc ccccaactcc accaacctca tcagcgactt tctcctgctg
     6121 ctgtgcgcct cccagcagtg gcaggtgttc tcagctgagc gcacagagga gtggcagcgc
     6181 atggctggcg tcaacaccga ccgcctggag ccgctgcggg gggagcccaa ccccgtgccc
     6241 aactttatcc actgcaggtc ctaccttgac atgctgaagg tggccgtctt ccgatacctg
     6301 ttctggctgg tgctggtggt ggtgtttgtc acgggggcca cccgcatcag catcttcggg
     6361 ctgggctacc tgctggcctg cttctacctg ctgctcttcg gcacggccct gctgcagagg
     6421 gacacacggg cccgcctcgt gctgtgggac tgcctcattc tgtacaacgt caccgtcatc
     6481 atctccaaga acatgctgtc gctcctggcc tgcgtcttcg tggagcagat gcagaccggc
     6541 ttctgctggg tcatccagct cttcagcctt gtatgcaccg tcaagggcta ctatgacccc
     6601 aaggagatga tggacagaga ccaggactgc ctgctgcctg tggaggaggc tggcatcatc
     6661 tgggacagcg tctgcttctt cttcctgctg ctgcagcgcc gcgtcttcct tagccattac
     6721 tacctgcacg tcagggccga cctccaggcc accgccctgc tagcctccag gggcttcgcc
     6781 ctctacaacg ctgccaacct caagagcatt gacttccacc gcaggataga ggagaagtcc
     6841 ctggcccagc tgaaaagaca gatggagcgt atccgtgcca agcaggagaa gcacaggcag
     6901 ggccgggtgg accgcagtcg cccccaggac accctgggcc ccaaggaccc cggcctggag
     6961 ccagggcccg acagtccagg gggctcctcc ccgccacgga ggcagtggtg gcggccctgg
     7021 ctggaccacg ccacagtcat ccactccggg gactacttcc tgtttgagtc cgacagtgag
     7081 gaagaggagg aggctgttcc tgaagacccg aggccgtcgg cacagagtgc cttccagctg
     7141 gcgtaccagg catgggtgac caacgcccag gcggtgctga ggcggcggca gcaggagcag
     7201 gagcaggcaa ggcaggaaca ggcaggacag ctacccacag gaggtggtcc cagccaggag
     7261 gtggagccag cagagggccc cgaggaggca gcggcaggcc ggagccatgt ggtgcagagg
     7321 gtgctgagca cggcgcagtt cctgtggatg ctggggcagg cgctagtgga tgagctgaca
     7381 cgctggctgc aggagttcac ccggcaccac ggcaccatga gcgacgtgct gcgggcagag
     7441 cgctacctcc tcacacagga gctcctgcag ggcggcgaag tgcacagggg cgtgctggat
     7501 cagctgtaca caagccaggc cgaggccacg ctgccaggcc ccaccgaggc ccccaatgcc
     7561 ccaagcaccg tgtccagtgg gctgggcgcg gaggagccac tcagcagcat gacagacgac
     7621 atgggcagcc ccctgagcac cggctaccac acgcgcagtg gcagtgagga ggcagtcacc
     7681 gaccccgggg agcgtgaggc tggtgcctct ctgtaccagg gactgatgcg gacggccagc
     7741 gagctgctcc tggacaggcg cctgcgcatc ccagagctgg aggaggcaga gctgtttgcg
     7801 gaggggcagg gccgggcgct gcggctgctg cgggccgtgt accagtgtgt ggccgcccac
     7861 tcggagctgc tctgctactt catcatcatc ctcaaccaca tggtcacggc ctccgccggc
     7921 tcgctggtgc tgcccgtgct cgtcttcctg tgggccatgc tgtcgatccc gaggcccagc
     7981 aagcgcttct ggatgacggc catcgtcttc accgagatcg cggtggtcgt caagtacctg
     8041 ttccagtttg ggttcttccc ctggaacagc cacgtggtgc tgcggcgcta cgagaacaag
     8101 ccctacttcc cgccccgcat cctgggcctg gagaagactg acggctacat caagtacgac
     8161 ctggtgcagc tcatggccct tttcttccac cgctcccagc tgctgtgcta tggcctctgg
     8221 gaccatgagg aggactcacc atccaaggag catgacaaga gcggcgagga ggagcaggga
     8281 gccgaggagg ggccaggggt gcctgcggcc accaccgaag accacattca ggtggaagcg
     8341 agggtcggac ccacggacgg gaccccagaa ccccaagtgg agctcaggcc ccgtgatacg
     8401 aggcgcatca gtctacgttt tagaagaagg aagaaggagg gcccagcacg gaaaggagcg
     8461 gcagccatcg aagctgagga cagggaggaa gaagaggggg aggaagagaa agaggccccc
     8521 acggggagag agaagaggcc aagccgctct ggaggaagag taagggcggc cgggcggcgg
     8581 ctgcagggct tctgcctgtc cctggcccag ggcacatatc ggccgctacg gcgcttcttc
     8641 cacgacatcc tgcacaccaa gtaccgcgca gccaccgacg tctatgccct catgttcctg
     8701 gctgatgttg tcgacttcat catcatcatt tttggcttct gggcctttgg gaagcactcg
     8761 gcggccacag acatcacgtc ctccctatca gacgaccagg tacccgaggc tttcctggtc
     8821 atgctgctga tccagttcag taccatggtg gttgaccgcg ccctctacct gcgcaagacc
     8881 gtgctgggca agctggcctt ccaggtggcg ctggtgctgg ccatccacct atggatgttc
     8941 ttcatcctgc ccgccgtcac tgagaggatg ttcaaccaga atgtggtggc ccagctctgg
     9001 tacttcgtga agtgcatcta cttcgccctg tccgcctacc agatccgctg cggctacccc
     9061 acccgcatcc tcggcaactt cctcaccaag aagtacaatc atctcaacct cttcctcttc
     9121 caggggttcc ggctggtgcc gttcctggtg gagctgcggg cagtgatgga ctgggtgtgg
     9181 acggacacca cgctgtccct gtccagctgg atgtgtgtgg aggacatcta tgccaacatc
     9241 ttcatcatca aatgcagccg agagacagag aagaaatacc cgcagcccaa agggcagaag
     9301 aagaagaaga tcgtcaagta cggcatgggt ggcctcatca tcctcttcct catcgccatc
     9361 atctggttcc cactgctctt catgtcgctg gtgcgctccg tggttggggt tgtcaaccag
     9421 cccatcgatg tcaccgtcac cctcaagctg ggcggctatg agccgctgtt caccatgagc
     9481 gcccagcagc cgtccatcat ccccttcacg gcccaggcct atgaggagct gtcccggcag
     9541 tttgaccccc agccgctggc catgcagttc atcagccagt acagccctga ggacatcgtc
     9601 acggcgcaga ttgagggcag ctccggggcg ctgtggcgca tcagtccccc cagccgtgcc
     9661 cagatgaagc gggagctcta caacggcacg gccgacatca ccctgcgctt cacctggaac
     9721 ttccagaggg acctggcgaa gggaggcact gtggagtatg ccaacgagaa gcacatgctg
     9781 gccctggccc ccaacagcac tgcacggcgg cagctggcca gcctgctcga gggcacctcg
     9841 gaccagtctg tggtcatccc caatctcttc cccaagtaca tccgtgcccc caacgggccc
     9901 gaagccaacc ctgtgaagca gctgcagccc aatgaggagg ccgactacct cggcgtgcgt
     9961 atccagctgc ggagggagca gggtgcgggg gccaccggct tcctcgaatg gtgggtcatc
    10021 gagctgcagg agtgccggac cgactgcaac ctgctgccca tggtcatttt cagtgacaag
    10081 gtcagcccac cgagcctcgg cttcctggct ggctacggca tcatggggct gtacgtgtcc
    10141 atcgtgctgg tcatcggcaa gttcgtgcgc ggattcttca gcgagatctc gcactccatt
    10201 atgttcgagg agctgccgtg cgtggaccgc atcctcaagc tctgccagga catcttcctg
    10261 gtgcgggaga ctcgggagct ggagctggag gaggagttgt acgccaagct catcttcctc
    10321 taccgctcac cggagaccat gatcaagtgg actcgtgaga aggagaccgg tggaggatcc
    10381 ggcatgggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa
    10441 atgggtcggg atctgtacga cgatgacgat aaggatctcg ccaccatggt cgactcatca
    10501 cgtcgtaagt acaataagac aggtcacgca gtcagagcta taggtcggct gagctcactc
    10561 gagaacgtct atatcaaggc cgacaagcag aagaacggca tcaaggcgaa cttccacatc
    10621 cgccacaaca tcgaggacgg cggcgtgcag ctcgcctacc actaccagca gaacaccccc
    10681 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcgtgca gtccaaactt
    10741 tcgaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
    10801 gggatcactc tcggcatgga cgagctgtac aagggcggta ccggagggag catggtgagc
    10861 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
    10921 aacggccaca agttcagcgt gtccggcgag ggtgagggcg atgccaccta cggcaagctg
    10981 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
    11041 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
    11101 ttcttcaagt ccgccatgcc cgaaggctac atccaggagc gcaccatctt cttcaaggac
    11161 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
    11221 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
    11281 tacaacctgc cggaccaact gactgaagag cagatcgcag aatttaaaga ggctttctcc
    11341 ctatttgaca aggacgggga tgggacaata acaaccaagg agctggggac ggtgatgcgg
    11401 tctctggggc agaaccccac agaagcagag ctgcaggaca tgatcaatga agtagatgcc
    11461 gacggtgacg gcacaatcga cttccctgag ttcctgacaa tgatggcaag aaaaatgaaa
    11521 tacagggaca cggaagaaga aattagagaa gcgttcggtg tgtttgataa ggatggcaat
    11581 ggctacatca gtgcagcaga gcttcgccac gtgatgacaa accttggaga gaagttaaca
    11641 gatgaagagg ttgatgaaat gatcagggaa gcagccatcg atggggatgg tcaggtaaac
    11701 tacgaagagt ttgtacaaat gatgacagcg aagtaaggaa ggaaaaaagc ggccgcgact
    11761 ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
    11821 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
    11881 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    11941 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa
    12001 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
    12061 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
    12121 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
    12181 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
    12241 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
    12301 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
    12361 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
    12421 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
    12481 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    12541 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa
    12601 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
    12661 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
    12721 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
    12781 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
    12841 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
    12901 gaagtagtga gg
//
