LOCUS       sequence-308751-        4838 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CaV1.3 5'UTR luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Kim JW, Yin X, Jhaldiyal A, Khan MR, Martin I, Xie Z, Perez-Rosello 
            T, Kumar M, Abalde-Atristain L, Xu J, Chen L, Eacker SM, Surmeier 
            DJ, Ingolia NT, Dawson TM, Dawson VL
  TITLE     Defects in mRNA Translation in LRRK2-Mutant hiPSC-Derived 
            Dopaminergic Neurons Lead to Dysregulated Calcium Homeostasis.
  JOURNAL   Cell Stem Cell. 2020 Oct 1;27(4):633-645.e7. doi: 
            10.1016/j.stem.2020.08.002. Epub 2020 Aug 25.
  PUBMED    32846140
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2020.08.002"; journalName: "Cell Stem Cell"; date: 
            "2020-10-1- 1"; volume: "27"; issue: "4"; pages: "633-645.e7"
COMMENT     Sequence Label: pJWK_VD_04
FEATURES             Location/Qualifiers
     source          1..4838
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..510
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     430..448
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             668..2320
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2361..2482
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2372..2391)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2426..2445
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2730..2747)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2901..3489)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2981..3000)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3689..4480,4481..4549))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4654..4702
                     /note="synthetic polyadenylation signal"
     misc_feature    4716..4807
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4756..4775
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
       61 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
      121 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
      181 tcgtccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
      241 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
      301 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
      361 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
      421 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
      481 cgttgaccga atcaccgacc tctctcccca aagcttggca atctggtact gttggtaaag
      541 ccaccaagga gaataagggc agggaccgcg gctcctacct cttggtgatc cccttcccca
      601 ttccgccccc gcctcaacgc ccagcacagt gccctgcaca cagtagtcgc tcaataaatg
      661 ttcgtggatg gaagatgcca aaaacattaa gaagggccca gcgccattct acccactcga
      721 agacgggacc gccggcgagc agctgcacaa agccatgaag cgctacgccc tggtgcccgg
      781 caccatcgcc tttaccgacg cacatatcga ggtggacatt acctacgccg agtacttcga
      841 gatgagcgtt cggctggcag aagctatgaa gcgctatggg ctgaatacaa accatcggat
      901 cgtggtgtgc agcgagaata gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat
      961 cggtgtggct gtggccccag ctaacgacat ctacaacgag cgcgagctgc tgaacagcat
     1021 gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa
     1081 cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc atggatagca agaccgacta
     1141 ccagggcttc caaagcatgt acaccttcgt gacttcccat ttgccacccg gcttcaacga
     1201 gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga tcatgaacag
     1261 tagtggcagt accggattgc ccaagggcgt agccctaccg caccgcaccg cttgtgtccg
     1321 attcagtcat gcccgcgacc ccatcttcgg caaccagatc atccccgaca ccgctatcct
     1381 cagcgtggtg ccatttcacc acggcttcgg catgttcacc acgctgggct acttgatctg
     1441 cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc gcagcttgca
     1501 agactataag attcaatctg ccctgctggt gcccacacta tttagcttct tcgctaagag
     1561 cactctcatc gacaagtacg acctaagcaa cttgcacgag atcgccagcg gcggggcgcc
     1621 gctcagcaag gaggtaggtg aggccgtggc caaacgcttc cacctaccag gcatccgcca
     1681 gggctacggc ctgacagaaa caaccagcgc cattctgatc acccccgaag gggacgacaa
     1741 gcctggcgca gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg acttggacac
     1801 cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat
     1861 gagcggctac gttaacaacc ccgaggctac aaacgctctc atcgacaagg acggctggct
     1921 gcacagcggc gacatcgcct actgggacga ggacgagcac ttcttcatcg tggaccggct
     1981 gaagagcctg atcaaataca agggctacca ggtagcccca gccgaactgg agagcatcct
     2041 gctgcaacac cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg
     2101 cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa accatgaccg agaaggagat
     2161 cgtggactat gtggccagcc aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt
     2221 cgtggacgag gtgcctaaag gactgaccgg caagttggac gcccgcaaga tccgcgagat
     2281 tctcattaag gccaagaagg gcggcaagat cgccgtgtaa ttctagagtc ggggcggccg
     2341 gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     2401 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     2461 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     2521 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga
     2581 taaggatccg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
     2641 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
     2701 gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2761 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     2821 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2881 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2941 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3001 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3061 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3121 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3181 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3241 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3301 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3361 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3421 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3481 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3541 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3601 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc
     3661 ggccgcaaat gctaaaccac tgcagtggtt accagtgctt gatcagtgag gcaccgatct
     3721 cagcgatctg cctatttcgt tcgtccatag tggcctgact ccccgtcgtg tagatcacta
     3781 cgattcgtga gggcttacca tcaggcccca gcgcagcaat gatgccgcga gagccgcgtt
     3841 caccggcccc cgatttgtca gcaatgaacc agccagcagg gagggccgag cgaagaagtg
     3901 gtcctgctac tttgtccgcc tccatccagt ctatgagctg ctgtcgtgat gctagagtaa
     3961 gaagttcgcc agtgagtagt ttccgaagag ttgtggccat tgctactggc atcgtggtat
     4021 cacgctcgtc gttcggtatg gcttcgttca actctggttc ccagcggtca agccgggtca
     4081 catgatcacc catattatga agaaatgcag tcagctcctt agggcctccg atcgttgtca
     4141 gaagtaagtt ggccgcggtg ttgtcgctca tggtaatggc agcactacac aattctctta
     4201 ccgtcatgcc atccgtaaga tgcttttccg tgaccggcga gtactcaacc aagtcgtttt
     4261 gtgagtagtg tatacggcga ccaagctgct cttgcccggc gtctatacgg gacaacaccg
     4321 cgccacatag cagtactttg aaagtgctca tcatcgggaa tcgttcttcg gggcggaaag
     4381 actcaaggat cttgccgcta ttgagatcca gttcgatata gcccactctt gcacccagtt
     4441 gatcttcagc atcttttact ttcaccagcg tttcggggtg tgcaaaaaca ggcaagcaaa
     4501 atgccgcaaa gaagggaatg agtgcgacac gaaaatgttg gatgctcata ctcgtccttt
     4561 ttcaatatta ttgaagcatt tatcagggtt actagtacgt ctctcaagga taagtaagta
     4621 atattaaggt acgggaggta ttggacaggc cgcaataaaa tatctttatt ttcattacat
     4681 ctgtgtgttg gttttttgtg tgaatcgata gtactaacat acgctctcca tcaaaacaaa
     4741 acgaaacaaa acaaactagc aaaataggct gtccccagtg caagtgcagg tgccagaaca
     4801 tttctctggc ctaactggcc ggtacctgag tcttccac
//
