LOCUS       sequence-308762-        1853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 ORF10 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1853)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046796
FEATURES             Location/Qualifiers
     source          1..1853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             203..223
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             224..247
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     rep_origin      368..956
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     857..876
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1062..1721
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1128..1147)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1792..1826
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag actcttctat gggctatata aacgttttcg cttttccgtt
      121 tacgatatat agtctactct tgtgcagaat gaattctcgt aactacatag cacaagtaga
      181 tgtagttaac tttaatctca cagagaacct gtactttcaa agctggagcc atccgcagtt
      241 tgaaaaataa ccggcttatc ggtcagtttc acctgattta cgtaaaaacc cgcttcggcg
      301 ggtttttgct tttggagggg cagaaagatg aatgactgtc cacgacgcta tacccaaaag
      361 aaaaagcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
      421 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
      481 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
      541 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
      601 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
      661 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
      721 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
      781 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
      841 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
      901 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaattga
      961 tcgggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat
     1021 tttttgagtt atcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
     1081 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
     1141 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
     1201 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
     1261 tgaatgctca tccggaattt cgtatggcaa tgaaagacgg tgagctggtg atatgggata
     1321 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
     1381 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
     1441 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtttcag
     1501 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     1561 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     1621 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
     1681 aattacaaca gtactgcgat gagtggcagg gcggggcgta atttgatatc gagctcgctt
     1741 ggactcctgt tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg
     1801 ctcggttgcc gccgggcgtt ttttattggt catcgatctc gatcccgcga aat
//
