LOCUS       sequence-308767-       10039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPaint frame selector +0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10039)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 10039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCAS9-mCherry-Frame +0
FEATURES             Location/Qualifiers
     source          1..10039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             946..1362
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1292..1314
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1330..1346
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        1373..1621
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1373..1393
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1544..1563
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        1642..1717
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        1758..2137
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2138..2341
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2292..2312
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2402..2411
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2411..2434
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2435..3139
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(2577..2595)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2845..2864
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3164..3217
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3218..7321
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3218..7321
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7334..7354
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     LTR             7533..8002
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(8132..8149)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8303..8891)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8383..8402)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9051..9842,9843..9911))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9674..9693
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9912..10016)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ctagttagct aactagctct gtatctggcg gacccgtggt
      661 ggaactgacg agttctgaac acccggccgc aaccctggga gacgtcccag ggactttggg
      721 ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga
      781 tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg
      841 ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg
      901 tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa attagggcca gactgttacc
      961 actcccttaa gtttgacctt aggtcactgg aaagatgtcg agcggatcgc tcacaaccag
     1021 tcggtagatg tcaagaagag acgttgggtt accttctgct ctgcagaatg gccaaccttt
     1081 aacgtcggat ggccgcgaga cggcaccttt aaccgagacc tcatcaccca ggttaagatc
     1141 aaggtctttt cacctggccc gcatggacac ccagaccagg tcccctacat cgtgacctgg
     1201 gaagccttgg cttttgaccc ccctccctgg gtcaagccct ttgtacaccc taagcctccg
     1261 cctcctcttc ctccatccgc cccgtctctc ccccttgaac ctcctcgttc gaccccgcct
     1321 cgatcctccc tttatccagc cctcactcct tctctaggcg ccggccggat ccgagggcct
     1381 atttcccatg attccttcat atttgcatat acgatacaag gctgttagag agataattgg
     1441 aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata
     1501 atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac
     1561 cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac
     1621 cgccagtacc caaaaagcgg ggttttagag ctagaaatag caagttaaaa taaggctagt
     1681 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttcgaatt ccgatgtacg
     1741 ggccagatat acgcgttgac attgattatt gtctagttat taatagtaat caattacggg
     1801 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     1861 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1921 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1981 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     2041 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     2101 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     2161 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     2221 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     2281 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     2341 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaagc ttccgcgtac
     2401 ggccaccatg gattataagg atgacgacga taaggtgagc aagggcgagg aggataacat
     2461 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     2521 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     2581 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     2641 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     2701 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     2761 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     2821 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     2881 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     2941 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     3001 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     3061 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     3121 catggacgag ctgtacaagg tcgctaccat cgagggatcc ggagagggca gaggaagtct
     3181 gctaacatgc ggtgacgtcg aggagaatcc tggcccaatg gacaagaagt acagcatcgg
     3241 cctggacatc ggcaccaaca gcgtgggctg ggccgtgatc accgacgagt acaaggtgcc
     3301 cagcaagaag ttcaaggtgc tgggcaacac cgaccgccac agcatcaaga agaacctgat
     3361 cggcgccctg ctgttcgaca gcggcgagac cgccgaggcc acccgcctga agcgcaccgc
     3421 ccgccgccgc tacacccgcc gcaagaaccg catctgctac ctgcaggaga tcttcagcaa
     3481 cgagatggcc aaggtggacg acagcttctt ccaccgcctg gaggagagct tcctggtgga
     3541 ggaggacaag aagcacgagc gccaccccat cttcggcaac atcgtggacg aggtggccta
     3601 ccacgagaag taccccacca tctaccacct gcgcaagaag ctggtggaca gcaccgacaa
     3661 ggccgacctg cgcctgatct acctggccct ggcccacatg atcaagttcc gcggccactt
     3721 cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt tcatccagct
     3781 ggtgcagacc tacaaccagc tgttcgagga gaaccccatc aacgccagcg gcgtggacgc
     3841 caaggccatc ctgagcgccc gcctgagcaa gagccgccgc ctggagaacc tgatcgccca
     3901 gctgcccggc gagaagaaga acggcctgtt cggcaacctg atcgccctga gcctgggcct
     3961 gacccccaac ttcaagagca acttcgacct ggccgaggac gccaagctgc agctgagcaa
     4021 ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc agtacgccga
     4081 cctgttcctg gccgccaaga acctgagcga cgccatcctg ctgagcgaca tcctgcgcgt
     4141 gaacaccgag atcaccaagg cccccctgag cgccagcatg atcaagcgct acgacgagca
     4201 ccaccaggac ctgaccctgc tgaaggccct ggtgcgccag cagctgcccg agaagtacaa
     4261 ggagatcttc ttcgaccaga gcaagaacgg ctacgccggc tacatcgacg gcggcgccag
     4321 ccaggaggag ttctacaagt tcatcaagcc catcctggag aagatggacg gcaccgagga
     4381 gctgctggtg aagctgaacc gcgaggacct gctgcgcaag cagcgcacct tcgacaacgg
     4441 cagcatcccc caccagatcc acctgggcga gctgcacgcc atcctgcgcc gccaggagga
     4501 cttctacccc ttcctgaagg acaaccgcga gaagatcgag aagatcctga ccttccgcat
     4561 cccctactac gtcggccccc tggcccgcgg caacagccgc ttcgcctgga tgacccgcaa
     4621 gagcgaggag accatcaccc cctggaactt cgaggaggtg gtggacaagg gcgccagcgc
     4681 ccagagcttc atcgagcgca tgaccaactt cgacaagaac ctgcccaacg agaaggtgct
     4741 gcccaagcac agcctgctgt acgagtactt caccgtgtac aacgagctga ccaaggtgaa
     4801 gtacgtgacc gagggcatgc gcaagcccgc cttcctgagc ggcgagcaga agaaggccat
     4861 cgtggacctg ctgttcaaga ccaaccgcaa ggtgaccgtg aagcagctga aggaggacta
     4921 cttcaagaag atcgagtgct tcgacagcgt ggagatcagc ggcgtggagg accgcttcaa
     4981 cgccagcctg ggcacctacc acgacctgct gaagatcatc aaggacaagg acttcctgga
     5041 caacgaggag aacgaggaca tcctggagga catcgtgctg accctgaccc tgttcgagga
     5101 ccgcgagatg atcgaggagc gcctgaagac ctacgcccac ctgttcgacg acaaggtgat
     5161 gaagcagctg aagcgccgcc gctacaccgg ctggggccgc ctgagccgca agctgatcaa
     5221 cggcatccgc gacaagcaga gcggcaagac catcctggac ttcctgaaga gcgacggctt
     5281 cgccaaccgc aacttcatgc agctgatcca cgacgacagc ctgaccttca aggaggacat
     5341 ccagaaggcc caggtgagcg gccagggcga cagcctgcac gagcacatcg ccaacctggc
     5401 cggcagcccc gccatcaaga agggcatcct gcagaccgtg aaggtggtgg acgagctggt
     5461 gaaggtgatg ggccgccaca agcccgagaa catcgtgatc gagatggccc gcgagaacca
     5521 gaccacccag aagggccaga agaacagccg cgagcgcatg aagcgcatcg aggagggcat
     5581 caaggagctg ggcagccaga tcctgaagga gcaccccgtg gagaacaccc agctgcagaa
     5641 cgagaagctg tacctgtact acctgcagaa cggccgcgac atgtacgtgg accaggagct
     5701 ggacatcaac cgcctgagcg actacgacgt ggaccacatc gtgccccaga gcttcctgaa
     5761 ggacgacagc atcgacaaca aggtgctgac ccgcagcgac aagaaccgcg gcaagagcga
     5821 caacgtgccc agcgaggagg tggtgaagaa gatgaagaac tactggcgcc agctgctgaa
     5881 cgccaagctg atcacccagc gcaagttcga caacctgacc aaggccgagc gcggcggcct
     5941 gagcgagctg gacaaggccg gcttcatcaa gcgccagctg gtggagaccc gccagatcac
     6001 caagcacgtg gcccagatcc tggacagccg catgaacacc aagtacgacg agaacgacaa
     6061 gctgatccgc gaggtgaagg tgatcaccct gaagagcaag ctggtgagcg acttccgcaa
     6121 ggacttccag ttctacaagg tgcgcgagat caacaactac caccacgccc acgacgccta
     6181 cctgaacgcc gtggtgggca ccgccctgat caagaagtac cccaagctgg agagcgagtt
     6241 cgtgtacggc gactacaagg tgtacgacgt gcgcaagatg atcgccaaga gcgagcagga
     6301 gatcggcaag gccaccgcca agtacttctt ctacagcaac atcatgaact tcttcaagac
     6361 cgagatcacc ctggccaacg gcgagatccg caagcgcccc ctgatcgaga ccaacggcga
     6421 gaccggcgag atcgtgtggg acaagggccg cgacttcgcc accgtgcgca aggtgctgag
     6481 catgccccag gtgaacatcg tgaagaagac cgaggtgcag accggcggct tcagcaagga
     6541 gagcatcctg cccaagcgca acagcgacaa gctgatcgcc cgcaagaagg actgggaccc
     6601 caagaagtac ggcggcttcg acagccccac cgtggcctac agcgtgctgg tggtggccaa
     6661 ggtggagaag ggcaagagca agaagctgaa gagcgtgaag gagctgctgg gcatcaccat
     6721 catggagcgc agcagcttcg agaagaaccc catcgacttc ctggaggcca agggctacaa
     6781 ggaggtgaag aaggacctga tcatcaagct gcccaagtac agcctgttcg agctggagaa
     6841 cggccgcaag cgcatgctgg ccagcgccgg cgagctgcag aagggcaacg agctggccct
     6901 gcccagcaag tacgtgaact tcctgtacct ggccagccac tacgagaagc tgaagggcag
     6961 ccccgaggac aacgagcaga agcagctgtt cgtggagcag cacaagcact acctggacga
     7021 gatcatcgag cagatcagcg agttcagcaa gcgcgtgatc ctggccgacg ccaacctgga
     7081 caaggtgctg agcgcctaca acaagcaccg cgacaagccc atccgcgagc aggccgagaa
     7141 catcatccac ctgttcaccc tgaccaacct gggcgccccc gccgccttca agtacttcga
     7201 caccaccatc gaccgcaagc gctacaccag caccaaggag gtgctggacg ccaccctgat
     7261 ccaccagagc atcaccggcc tgtacgagac ccgcatcgac ctgagccagc tgggcggcga
     7321 cagccgcgcc gaccccaaga agaagcgcaa ggtgtgagcg gccgcatcga taaaataaaa
     7381 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     7441 gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa
     7501 gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt
     7561 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc
     7621 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc
     7681 ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc
     7741 caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt
     7801 ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg
     7861 cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc
     7921 agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact
     7981 acccgtcagc gggggtcttt cacatgcagc atgtatcaaa attaatttgg ttttttttct
     8041 taagtattta cattaaatgg ccatagttgc attaatgaat cggccaacgc gcggggagag
     8101 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     8161 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     8221 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     8281 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     8341 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     8401 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8461 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8521 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8581 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8641 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8701 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     8761 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8821 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8881 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8941 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9001 tgcggccgca aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9061 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9121 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9181 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9241 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9301 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9361 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9421 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9481 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9541 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9601 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9661 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9721 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9781 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9841 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9901 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9961 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10021 tttccccgaa aagtgccac
//
