LOCUS       sequence-308773-       10972 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  empty GFP vector for cloning sgRNA or pgRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10972)
  AUTHORS   Esposito R, Polidori T, Meise DF, Pulido-Quetglas C, Chouvardas P, 
            Forster S, Schaerer P, Kobel A, Schlatter J, Kerkhof E, Roemmele M, 
            Rice ES, Zhu L, Lanzos A, Guillen-Ramirez HA, Basile G, Carrozzo I, 
            Vancura A, Ullrich S, Andrades A, Harvey D, Medina PP, Ma PC, 
            Haefliger S, Wang X, Martinez I, Ochsenbein AF, Riether C, Johnson R
  TITLE     Multi-hallmark long noncoding RNA maps reveal non-small cell lung 
            cancer vulnerabilities.
  JOURNAL   Cell Genom. 2022 Aug 22;2(9):100171. doi: 
            10.1016/j.xgen.2022.100171. eCollection 2022 Sep 14.
  PUBMED    36778670
REFERENCE   2  (bases 1 to 10972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Genom."; date: "2022-08-22"; volume: "2(9):100171. doi"; pages: " 
            10.1016/j.xgen.2022.100171. eCollection 2022 Sep 14"
COMMENT     Sequence Label: pDECKO_GFP
FEATURES             Location/Qualifiers
     source          1..10972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2131..2206
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    2262..2379
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2518..3696
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2749..3687
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3644..3664
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3709..4305
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3709..3728)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4205..4225
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4315..4368
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4375..4384
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4381..4383,4384..4386,4387..5097)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKLSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4426..4447)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4687..4706)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5034..5055
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5113..5701
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5166..5186)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5584..5595)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5773..6006
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6084..6205
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6121..6140)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6175..6194
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6245..6380
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6400..6419)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6401..6419)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6429..6446)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6429..6445)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6438..6460)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6587..7042
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6674..6693)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6884..6905
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7068..7172
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7173..7241,7242..8033)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7391..7410)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8204..8792
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8693..8712
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8946..8963
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9080..9101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9116..9121,9122..9139,9140..9146)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9154..9170
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9159..9181
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9178..9194
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9178..9194
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9213..9233
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9215..9233
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9261..9487
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9488..9668
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9715..9840
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10333..10566
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10751..10795
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg gagacggttg taaatgagca cacaaaatac acatgctaaa atattatatt
      301 ctatgacctt tataaaatca accaaaatct tctttttaat aactttagta tcaataatta
      361 gaatttttat gttccttttt gcaaactttt aataaaaatg agcaaaataa aaaaacgcta
      421 gttttagtaa ctcgcgttgt tttcttcacc tttaataata gctactccac cacttgttcc
      481 taagcggtca gctcctgctt caatcatttt ttgagcatct tcaaatgttc taactccacc
      541 agctgcttta actaaagcat tgtctttaac aactgacttc attagtttaa catcttcaaa
      601 tgttgcacct gattttgaaa atcctgttga tgttttaaca aattctaatc cagcttcaac
      661 agctatttca caagctttca tgatttcttc ttttgttaat aaacaatttt ccataataca
      721 tttaacaaca tgtgatccag ctgctttttt tacagctttc atgtcttcta aaactaattc
      781 ataatttttg tcttttaatg caccaatatt taataccata tcaatttctg ttgcaccatc
      841 tttaattgct tcagaaactt cgaatgcttt tgtagctgtt gtgcatgcac ctagaggaaa
      901 acctacaaca tttgttattc ctacatttgt gccttttaat aattctttac aatagcttgt
      961 tcaatatgaa ttaacacaaa ctgttgcaaa atcaaattca attgcttcat cacataattg
     1021 tttaatttca gctttcgtag catcttgttt taataatgtg tgatctatat atttgtttag
     1081 tttcattttt tctcctatat attcattttt aattttaatt ctttaataat ttcgtctact
     1141 ttaactttag cgttttgaac agattcacca acacctataa aataaatttt tagtttaggt
     1201 tcagttccac ttgggcgaac agcaaatcat gacttatctt ctaaataaaa ttttagtaag
     1261 tcttgtcctg gcatattata cattccatcg atgtagtctt caacattaac aactttaagt
     1321 ccagcaattt gagttaaggg tgttgctctc aatgatttca ttaatggttc aatttttaat
     1381 ttcttttctt ctggtttaaa attcaagttt aaagtgaaag tgtaatatgc acccatttct
     1441 ttaaataaat cttctaaata gtctactaat gttttatttt gttttttata aaatcaagca
     1501 gcctctgcta ttaatataga agcttgtatt ccatctttat ctctagctga gtcatcaatt
     1561 acatatccat aactttcttc ataagcaaaa acaaaattta atccgttatc ttcttcttta
     1621 gcaatttctc tacccattca tttaaatcca gttaaagttt ttacaatatt aactccatat
     1681 ttttcatgag cgattctatc acccaaatca cttgttacaa aacttgaata tagagccgga
     1741 ttttttggaa tgctatttaa gcgttttaga tttgataatt ttcaatcaat taaaattggt
     1801 cctgtttgat ttccatctaa tcttacaaaa tgaccatcat gttttattgc cattccaaat
     1861 ctgtcagcat ctgggtcatt cataataata atatctgcat catgtttaat accatattca
     1921 agcggtattt ttcatgcagg atcaaattct ggatttggat ttacaacatt tttaaatgtt
     1981 tcatcttcaa atgcatgctc ttcaacctca ataacgttat atcctgattc acgtaatatt
     2041 tttggggtaa atttagttcc tgttccatta actgcgctaa aaataatttt taaatctttt
     2101 ttagcttctt gctctttttt gtacgtctct gttttagagc tagaaatagc aagttaaaat
     2161 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttaagcttgg
     2221 cgtaactaga tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
     2281 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     2341 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     2401 agagatccac tttggcgccg gctcgagggg gcccgggtgc aaagatggat aaagttttaa
     2461 acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt gggaattggc
     2521 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     2581 ggggtcggca attgatccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     2641 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     2701 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg
     2761 tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact
     2821 tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag
     2881 agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct
     2941 gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc
     3001 gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa
     3061 gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg
     3121 ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg
     3181 cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc
     3241 ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt
     3301 gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg
     3361 cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc
     3421 tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag
     3481 ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag
     3541 tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc
     3601 tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg
     3661 gttcaaagtt tttttcttcc atttcaggtg tcgtgacgta cggccaccat gaccgagtac
     3721 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     3781 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     3841 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     3901 tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg
     3961 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     4021 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     4081 ctggccaccg tcggagtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     4141 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     4201 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     4261 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccggatc gggagagggc
     4321 agaggaagtc tgctaacatg cggtgacgtc gaggagaatc ctggcccacc ggtcgccacc
     4381 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     4441 ggcgacgtaa acggccacaa gctcagcgtg tccggcgagg gcgagggcga tgccacctac
     4501 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     4561 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     4621 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     4681 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     4741 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     4801 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     4861 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     4921 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     4981 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     5041 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagacg
     5101 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     5161 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     5221 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     5281 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     5341 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     5401 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     5461 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     5521 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     5581 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     5641 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     5701 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     5761 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     5821 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     5881 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     5941 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6001 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     6061 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     6121 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     6181 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     6241 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     6301 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     6361 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     6421 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     6481 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     6541 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     6601 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     6661 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     6721 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     6781 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     6841 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     6901 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     6961 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     7021 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7081 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7141 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7201 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7261 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     7321 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     7381 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     7441 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7501 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7561 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7621 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7681 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7741 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7801 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7861 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7921 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7981 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     8041 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     8101 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     8161 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     8221 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8281 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     8341 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8401 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8461 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8521 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8581 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8641 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8701 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8761 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8821 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     8881 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     8941 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     9001 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     9061 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     9121 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     9181 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     9241 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     9301 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     9361 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     9421 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     9481 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     9541 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     9601 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     9661 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     9721 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     9781 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     9841 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     9901 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     9961 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
    10021 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
    10081 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
    10141 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
    10201 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
    10261 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
    10321 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
    10381 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
    10441 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
    10501 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
    10561 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
    10621 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
    10681 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
    10741 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
    10801 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
    10861 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
    10921 atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccca ga
//
