LOCUS       sequence-308777-        9431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  targeting, non-essential locus control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   Esposito R, Polidori T, Meise DF, Pulido-Quetglas C, Chouvardas P, 
            Forster S, Schaerer P, Kobel A, Schlatter J, Kerkhof E, Roemmele M, 
            Rice ES, Zhu L, Lanzos A, Guillen-Ramirez HA, Basile G, Carrozzo I, 
            Vancura A, Ullrich S, Andrades A, Harvey D, Medina PP, Ma PC, 
            Haefliger S, Wang X, Martinez I, Ochsenbein AF, Riether C, Johnson R
  TITLE     Multi-hallmark long noncoding RNA maps reveal non-small cell lung 
            cancer vulnerabilities.
  JOURNAL   Cell Genom. 2022 Aug 22;2(9):100171. doi: 
            10.1016/j.xgen.2022.100171. eCollection 2022 Sep 14.
  PUBMED    36778670
REFERENCE   2  (bases 1 to 9431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Genom."; date: "2022-08-22"; volume: "2(9):100171. doi"; pages: " 
            10.1016/j.xgen.2022.100171. eCollection 2022 Sep 14"
COMMENT     Sequence Label: pDECKO_mCherry_AAVS1_1801
FEATURES             Location/Qualifiers
     source          1..9431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..45
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        223..471
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     223..243
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     394..413
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        493..568
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        575..789
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     710..729
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        818..893
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    949..1066
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1205..2383
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1436..2374
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2331..2351
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2396..2992
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2396..2415)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2892..2912
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3002..3055
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      3062..3071
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3068..3778
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3213..3231)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3481..3500
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3794..4382
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3847..3867)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4265..4276)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4454..4687
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4765..4886
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4802..4821)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4856..4875
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4926..5061
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5081..5100)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5082..5100)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5110..5127)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5110..5126)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5119..5141)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5268..5723
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5355..5374)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5565..5586
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5749..5853
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5854..5922,5923..6714)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6072..6091)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6885..7473
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7374..7393
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7627..7644
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7761..7782
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7797..7802,7803..7820,7821..7827)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7835..7851
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7840..7862
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7859..7875
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7859..7875
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7894..7914
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7896..7914
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7942..8168
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8169..8349
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8396..8521
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9014..9247
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
       61 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
      121 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
      181 ttatcgtttc agacccacct cccaaccccg aggggaccca gagagggcct atttcccatg
      241 attccttcat atttgcatat acgatacaag gctgttagag agataattag aattaatttg
      301 actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg
      361 tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga
      421 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac cggagtgccc
      481 ttgctgtgcc gcgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
      541 acttgaaaaa gtggcaccga gtcggtgctt ttttgaacgc tgacgtcatc aacccgctcc
      601 aaggaatcgc gggcccagtg tcactaggcg ggaacaccca gcgcgcgtgc gccctggcag
      661 gaagatggct gtgagggaca ggggagtggc gccctgcaat atttgcatgt cgctatgtgt
      721 tctgggaaat caccataaac gtgaaatgtc tttggatttg ggagtcttat aagttctgta
      781 tgagaccact ctttccccct ctgggggatg caggggagtt ttagagctag aaatagcaag
      841 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgctttttta
      901 agcttggcgt aactagatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa
      961 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     1021 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     1081 ggacagcaga gatccacttt ggcgccggct cgagggggcc cgggtgcaaa gatggataaa
     1141 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
     1201 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     1261 ggggggaggg gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga
     1321 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     1381 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     1441 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     1501 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     1561 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     1621 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     1681 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     1741 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     1801 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     1861 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     1921 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     1981 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     2041 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     2101 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     2161 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     2221 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     2281 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     2341 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgacgtacgg ccaccatgac
     2401 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     2461 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     2521 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     2581 caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt
     2641 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     2701 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     2761 gtggttcctg gccaccgtcg gagtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     2821 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     2881 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     2941 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccggatcggg
     3001 agagggcaga ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg gcccaccggt
     3061 cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
     3121 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     3181 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     3241 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     3301 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
     3361 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     3421 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     3481 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     3541 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     3601 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     3661 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     3721 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaaac
     3781 gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     3841 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     3901 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     3961 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     4021 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     4081 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     4141 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     4201 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     4261 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     4321 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     4381 gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     4441 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     4501 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     4561 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     4621 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     4681 tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     4741 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     4801 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     4861 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     4921 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     4981 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     5041 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     5101 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     5161 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     5221 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     5281 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     5341 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     5401 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     5461 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     5521 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     5581 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     5641 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     5701 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     5761 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5821 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5881 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5941 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     6001 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     6061 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     6121 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     6181 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     6241 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     6301 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     6361 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     6421 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     6481 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     6541 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6601 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6661 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6721 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6781 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6841 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6901 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6961 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     7021 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     7081 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     7141 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     7201 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7261 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7321 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7381 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7441 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7501 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     7561 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     7621 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     7681 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     7741 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     7801 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     7861 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     7921 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     7981 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
     8041 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     8101 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
     8161 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     8221 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     8281 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8341 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     8401 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     8461 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     8521 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     8581 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     8641 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     8701 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     8761 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     8821 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     8881 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     8941 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     9001 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     9061 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     9121 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     9181 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     9241 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     9301 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     9361 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     9421 accagcaaga a
//
