LOCUS       sequence-308781-        3321 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of SARS-CoV-2 nsp7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Hillen HS, Kokic G, Farnung L, Dienemann C, Tegunov D, Cramer P
  TITLE     Structure of replicating SARS-CoV-2 polymerase.
  JOURNAL   Nature. 2020 May 21. pii: 10.1038/s41586-020-2368-8. doi: 
            10.1038/s41586-020-2368-8.
  PUBMED    32438371
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2020 May 21. pii: 10.1038/s41586-020-2368-8. doi: 
            10.1038/s41586-020-2368-8."
COMMENT     Sequence Label: pET-His-SCov2-nsp7
FEATURES             Location/Qualifiers
     source          1..3321
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             36..58
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             77..94
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     terminator      416..462
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      1629..1668
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1699..2287
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2188..2207
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2441..2458
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2473..2612
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2538..2557)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2698..2720
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2714..2905)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      3163..3209
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3269..3296
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 taatacgact cactataggc ccctctagaa ataattttgt ttaactttaa gaaggagata
       61 taccatgggt tcttctcacc atcaccatca ccatgaaaac ctgtacttcc aatccaatgc
      121 aagcaaaatg tccgatgtta aatgcaccag cgttgttctg ctgagcgttc tgcagcagct
      181 gcgtgttgaa agcagcagca aactgtgggc acagtgtgtt cagctgcata atgatattct
      241 gctggcaaaa gataccaccg aagcctttga aaaaatggtt agcctgctga gtgtgctgct
      301 gagcatgcag ggtgcagttg atattaacaa actgtgtgaa gagatgctgg ataatcgtgc
      361 aaccctgcag taataacatt ggaagtggat aactggctgc tgccaccgct gagcatagca
      421 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
      481 tcaagtcccg aagtagctgt cagactgctg gcgaccgccg caactagtgg cgtcatgcgt
      541 gccgtatggt ctttcgcctg tagacgccct acaagccgta ctaaagtgga aagacccgca
      601 aaaggtatcg cgatcgccct gcagggaact atatccgact agtagcccgc ctaatgagcg
      661 ggcttttttt taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      721 tcatgagaca ataaccctga taaatgcttc gaaaaaggaa gagtatgagt attcaacatt
      781 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
      841 aaacgctcgt gaaagtaaaa gacgcagagg accaattggg ggcacgagtg ggatacatag
      901 aactggactt gaatagcggt aaaatccttg agagttttcg ccctgaagag cgttttccaa
      961 tgatgagcac tttcaaagtt ctgctatgtg gagcagtatt atcccgtgta gatgcggggc
     1021 aagagcaact cggacgacga atacactatt cgcagaatga cttggttgaa tactccccag
     1081 tgacagaaaa gcaccttacg gacggaatga cggtaagaga attatgtagt gccgccataa
     1141 cgatgagtga taacactgcg gcgaacttac ttctgacaac catcggtgga ccgaaggaat
     1201 taaccgcttt tttgcacaat atgggagacc atgtaactcg ccttgaccgt tgggaaccag
     1261 aactgaatga agccatacca aacgacgagc gagacaccac aatgcctgcg gcaatggcaa
     1321 caacattacg caaactatta actggcgaac tacttactct ggcttcacgg caacaattaa
     1381 tagactggct tgaagcggat aaagttgcag gaccactact gcgttcggca cttcctgctg
     1441 gctggtttat tgctgataaa tctggggcag gagagcgtgg ttcacggggt atcattgccg
     1501 cacttggacc agatggtaag ccttcccgta tcgtagttat ctacacgacg ggtagtcagg
     1561 caactatgga cgaacgaaat agacagattg ctgaaatagg ggcttcactg attaagcatt
     1621 ggtaaaccga tacaattaaa ggctcctttt ggagcctttt tttttggacg gaccggtaga
     1681 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     1741 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1801 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     1861 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     1921 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     1981 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     2041 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     2101 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     2161 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2221 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     2281 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     2341 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcccttga
     2401 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     2461 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     2521 caatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
     2581 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     2641 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     2701 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt
     2761 aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca
     2821 gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa
     2881 gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg
     2941 ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca
     3001 tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca
     3061 gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttggcac
     3121 catgcatcga tcccgcgaaa tgcggccgct taattaaggc atcaaataaa acgaaaggct
     3181 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgagtcc ttccagtctg
     3241 ggcgaaagcg cggtcgatca aattaagcag aaggccatcc tgacggatgg cctttttgcg
     3301 tttctatcga tcccgcgaaa t
//
