LOCUS       sequence-308783-        9601 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9601)
  AUTHORS   Hanna RE, Hegde M, Fagre CR, DeWeirdt PC, Sangree AK, Szegletes Z, 
            Griffith A, Feeley MN, Sanson KR, Baidi Y, Koblan LW, Liu DR, Neal 
            JT, Doench JG
  TITLE     Massively parallel assessment of human variants with base editor 
            screens.
  JOURNAL   Cell. 2021 Feb 18;184(4):1064-1080.e20. doi: 
            10.1016/j.cell.2021.01.012.
  PUBMED    33606977
REFERENCE   2  (bases 1 to 9601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.01"; journalName: "Cell"; date: "2021-02-18- 
            18"; volume: "184"; issue: "4"; pages: "1064-1080.e20"
COMMENT     Sequence Label: pLX_313-EGFP
FEATURES             Location/Qualifiers
     source          1..9601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     178..197
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     437..458
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(524..548)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(568..1746)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          577..1515
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(600..620)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    1884..2001
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2072..3091)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     promoter        complement(3113..3309)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(3121..3331)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3127..3262
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(3562..3606)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3791..4024
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4517..4642
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4689..4869
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4870..5096)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(5124..5144)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5124..5142)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5163..5179)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5163..5179)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5176..5198)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5187..5203
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5211..5217,5218..5235,5236..5241))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5256..5277
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5394..5411)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5565..6153)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5645..5664)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6324..7115,7116..7184))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6947..6966
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7185..7289)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7315..7770)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7452..7473)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7664..7683
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7897..7919
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7911..7928
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7912..7928
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7938..7957
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7938..7956
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      7977..8112
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8152..8273
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8163..8182)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8217..8236
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             8351..8584
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    8656..9244
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             8762..8773
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     9171..9191
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9295..9336)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    9344..9368
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(9388..9601,1..497,498..500,501..503))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9430..9451)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atcttgaagt tcgccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg
       61 ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
      121 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
      181 ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg
      241 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg
      301 ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag
      361 atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc agacacgctg
      421 aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac
      481 agctcctcgc ccttgctcac catggtgaag ggggcggccg cggagcctgc ttttttgtac
      541 aaacttgttg atcaatcgat gctagcctca cgacacctga aatggaagaa aaaaactttg
      601 aaccactgtc tgaggcttga gaatgaacca agatccaaac tcaaaaaggg caaattccaa
      661 ggagaattac atcaagtgcc aagctggcct aacttcagtc tccacccact cagtgtgggg
      721 aaactccatc gcataaaacc cctcccccca acctaaagac gacgtactcc aaaagctcga
      781 gaactaatcg aggtgcctgg acggcgcccg gtactccgtg gagtcacatg aagcgacggc
      841 tgaggacgga aaggcccttt tcctttgtgt gggtgactca cccgcccgct ctcccgagcg
      901 ccgcgtcctc cattttgagc tccctgcagc agggccggga agcggccatc tttccgctca
      961 cgcaactggt gccgaccggg ccagccttgc cgcccagggc ggggcgatac acggcggcgc
     1021 gaggccaggc accagagcag gccggccagc ttgagactac ccccgtccga ttctcggtgg
     1081 ccgcgctcgc aggccccgcc tcgccgaaca tgtgcgctgg gacgcacggg ccccgtcgcc
     1141 gcccgcggcc ccaaaaaccg aaataccagt gtgcagatct tggcccgcat ttacaagact
     1201 atcttgccag aaaaaaagcg tcgcagcagg tcatcaaaaa ttttaaatgg ctagagactt
     1261 atcgaaagca gcgagacagg cgcgaaggtg ccaccagatt cgcacgcggc ggccccagcg
     1321 cccaggccag gcctcaactc aagcacgagg cgaaggggct ccttaagcgc aaggcctcga
     1381 actctcccac ccacttccaa cccgaagctc gggatcaaga atcacgtact gcagccaggt
     1441 ggaagtaatt caaggcacgc aagggccata acccgtaaag aggccaggcc cgcgggaacc
     1501 acacacggca cttacctgtg ttctggcggc aaacccgttg cgaaaaagaa cgttcacggc
     1561 gactactgca cttatatacg gttctccccc accctcggga aaaaggcgga gccagtacac
     1621 gacatcactt tcccagttta ccccgcgcca ccttctctag gcaccggttc aattgccgac
     1681 ccctcccccc aacttctcgg ggactgtggg cgatgtgcgc tctgcccact gacgggcacc
     1741 ggagccaatt cccactcctt tcaagaccta gaaggtccat tagctgcaaa gattcctctc
     1801 tgtttaaaac tttatccatc tttgcacctg caggcctcga gccggcgcca aagtggatct
     1861 ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg taatttgttt ttgtaattct
     1921 ttagtttgta tgtctgttgc tattatgtct actattcttt cccctgcact gtacccccca
     1981 atcccccctt ttcttttaaa attgtggatg aatactgcca tttgtctcaa gatctagaat
     2041 tcgttaacgg cgcgcctcat tactgcagat cctttgccct aggacgagtg ctggggcgtc
     2101 tgtttccact atcggcgagc acttccacac agccatcggt ccagacggca gcgcttctgc
     2161 gggcgatttg tgtacgcccg acagtcccgg ctccggaccg gacgattgcg tcgcagcgac
     2221 cctgagccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa
     2281 gaccaatgcg gagcatataa gcccggagcc gaggagatcc tgcaagctcg ggatgcctcc
     2341 gctcgaagta gcgggtctgc tgctccatac aagccaacca gggcctccag aagaagatgt
     2401 tggcgacctc gtattgggaa tccccgaaca tggcctcgct ccagtcaatg acggctgtta
     2461 tgcggccatt gtcggtcagg acattgttgg agccgaaatc ggcgtgcacg aggtgccgga
     2521 cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgggcg acggaggcac
     2581 tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca atagcgcaga
     2641 tgaaatcgcg ccatgtagtg tattgaccga ttccttgagg tccgaatggg ccgaacccgc
     2701 tggtctggct aagatcggcg gcagcgatgg catccatggc ctcggcgacg ggctggagaa
     2761 cagcgggcag ttcggtttca ggcaggtctt gcaaggtgac accctgtgca cggcgggaga
     2821 tgcaataggt caggctctcg ctgaactccc caatgtcaag cacttcggga atagggagag
     2881 cggcggatgc aaagtgccga taaacatagc gatctttgta gaaaccatcg gcgcagctat
     2941 tcacccgcag gacatatcca cgccctccca catcgaagct gaaagcccga gattcttcgc
     3001 cctcggagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga
     3061 cagaggtagc ggtgagttca ggctttttca tggtggtacc tctagaaagc tttttgcaaa
     3121 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg
     3181 cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac
     3241 tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat
     3301 tgagacgcat gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg
     3361 ttgctgacta attgagatgc aatgcatggg tcccctcggg gttgggaggt gggtctgaaa
     3421 cgataatggt gaatatccct gcctaactct attcactata gaaagtacag caaaaactat
     3481 tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt
     3541 tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc
     3601 attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc ttgtgtaatt
     3661 gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt ccagagattt
     3721 attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc cagagcaacc
     3781 ccaaatcccc aggagctgtt gatcctttag gtatctttcc acagccagga ttcttgcctg
     3841 gagctgcttg atgccccaga ctgtgagttg caacagatgc tgttgcgcct caatagccct
     3901 cagcaaattg ttctgctgct gcactatacc agacaataat tgtctggcct gtaccgtcag
     3961 cgtcattgac gctgcgccca tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc
     4021 tcctattccc actgctcttt tttctctctg caccactctt ctctttgcct tggtgggtgc
     4081 tactcctaat ggttcaattt ttactacttt atatttatat aattcacttc tccaattgtc
     4141 cctcatatct cctcctccag gtctgaagat cagcggccgc ttgctgtgcg gtggtcttac
     4201 ttttgttttg ctcttcctct atcttgtcta aagcttcctt ggtgtctttt atctctatcc
     4261 tttgatgcac acaatagagg gttgctactg tattatataa tgatctaagt tcttctgatc
     4321 ctgtctgaag ggatggttgt agctgtccca gtatttgtct acagccttct gatgtttcta
     4381 acaggccagg attaactgcg aatcgttcta gctccctgct tgcccatact atatgtttta
     4441 atttatattt tttctttccc cctggcctta accgaatttt ttcccatcgc gatctaattc
     4501 tcccccgctt aatactgacg ctctcgcacc catctctctc cttctagcct ccgctagtca
     4561 aaatttttgg cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa
     4621 gccgagtcct gcgtcgagag agctcctctg gtttcccttt cgctttcaag tccctgttcg
     4681 ggcgccactg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc
     4741 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg
     4801 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
     4861 gagagacccg tttatgtatc gagctaggca cttaaataca atatctctgc aatgcggcaa
     4921 ttcagtggtt cgtccaatcc atgtcagacc cgtctgttgc cttcctaata aggcacgatc
     4981 gtaccacctt acttccacca atcggcatgc acggtgcttt ttctctcctt gtaaggcatg
     5041 ttgctaactc atcgttacca tgttgcaaga ctacaagagt attgcataag actacattaa
     5101 gcttgcagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
     5161 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     5221 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     5281 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     5341 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     5401 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     5461 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     5521 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5581 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     5641 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     5701 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     5761 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     5821 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     5881 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     5941 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6001 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6061 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6121 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6181 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6241 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6301 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     6361 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6421 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     6481 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     6541 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     6601 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     6661 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     6721 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     6781 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     6841 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     6901 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     6961 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7021 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7081 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7141 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7201 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7261 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg
     7321 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     7381 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     7441 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     7501 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     7561 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     7621 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     7681 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     7741 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt
     7801 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     7861 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     7921 cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacg tccctaggcc
     7981 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     8041 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     8101 taggggcggg atgggcggag ttaggggcgg gatagctaga gccagacatg ataagataca
     8161 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     8221 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttcctctca
     8281 ctctctgata ttcatttctt tgcaagttat aaatactgaa taataagatg acatgaacta
     8341 ctatacgtac tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta
     8401 ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga
     8461 ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac
     8521 cagagagacc cagtacaagc aaaaagcaga tcttgtcttc gttgggagtg aattagccct
     8581 tccagtcccc ccttttcttt taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt
     8641 ggtcttaaag tcgacgcggg gaggcggccc aaagggagat ccgactcgtc tgagggcgaa
     8701 ggcgaagacg cggaagaggc cgcagagccg gcagcaggcc gcgggaagga aggtccgctg
     8761 gattgagggc cgaagggacg tagcagaagg acgtcccgcg cagaatccag gtggcaacac
     8821 aggcgagcag ccaaggaaag gacgatgatt tccccgacaa caccacggaa ttgtcagtgc
     8881 ccaacagccg agcccctgtc cagcagcggg caaggcaggc ggcgatgagt tccgccgtgg
     8941 caatagggag ggggaaagcg aaagtcccgg aaaggagctg acaggtggtg gcaatgcccc
     9001 aaccagtggg ggttgcgtca gcaaacacag tgcacaccac gccacgttgc ctgacaacgg
     9061 gccacaactc ctcataaaga gacagcaacc aggatttata caaggaggag aaaatgaaag
     9121 ccatacggga agcaatagca tgatacaaag gcattaaagc agcgtatcca catagcgtaa
     9181 aaggagcaac atagttaaga ataccagtca atctttcaca aattttgtaa tccagaggtt
     9241 gattgtcgac ttaacgcgtt gattgtcgac ttaaccggta ctagttcatt actacgtaga
     9301 atcgagaccg aggagagggt tagggatagg cttaccgata tcaaccactt tgtacaagaa
     9361 agctgggtcg gcgcgcccac ccttggactt gtacagctcg tccatgccga gagtgatccc
     9421 ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct
     9481 cagggcggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat
     9541 gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg
     9601 g
//
