LOCUS       sequence-308784-        9438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  targeting, non-essential locus control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9438)
  TITLE     Johnson lab sgRNA plasmids
REFERENCE   2  (bases 1 to 9438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDECKO_GFP_AAVS1_186
FEATURES             Location/Qualifiers
     source          1..9438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..45
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        223..471
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     223..243
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     394..413
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        494..569
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        576..790
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     711..730
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        819..894
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    950..1067
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1206..2384
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1437..2375
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2332..2352
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2397..2993
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2397..2416)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2893..2913
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3003..3056
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      3063..3072
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3069..3071,3072..3074,3075..3785)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKLSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3114..3135)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3375..3394)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3722..3743
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3801..4389
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3854..3874)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4272..4283)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4461..4694
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4772..4893
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4809..4828)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4863..4882
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4933..5068
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5088..5107)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5089..5107)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5117..5134)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5117..5133)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5126..5148)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5275..5730
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5362..5381)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5572..5593
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5756..5860
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5861..5929,5930..6721)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6079..6098)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6892..7480
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7381..7400
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7634..7651
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7768..7789
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7804..7809,7810..7827,7828..7834)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7842..7858
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7847..7869
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7866..7882
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7866..7882
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7901..7921
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7903..7921
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7949..8175
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8176..8356
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8403..8528
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9021..9254
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
       61 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
      121 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
      181 ttatcgtttc agacccacct cccaaccccg aggggaccca gagagggcct atttcccatg
      241 attccttcat atttgcatat acgatacaag gctgttagag agataattag aattaatttg
      301 actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg
      361 tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga
      421 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac cggactcttt
      481 aaggaaagaa ggagttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
      541 aacttgaaaa agtggcaccg agtcggtgct tttttgaacg ctgacgtcat caacccgctc
      601 caaggaatcg cgggcccagt gtcactaggc gggaacaccc agcgcgcgtg cgccctggca
      661 ggaagatggc tgtgagggac aggggagtgg cgccctgcaa tatttgcatg tcgctatgtg
      721 ttctgggaaa tcaccataaa cgtgaaatgt ctttggattt gggagtctta taagttctgt
      781 atgagaccac tctttccctc agtctgaaga gcagagccgt tttagagcta gaaatagcaa
      841 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
      901 aagcttggcg taactagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
      961 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     1021 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     1081 gggacagcag agatccactt tggcgccggc tcgagggggc ccgggtgcaa agatggataa
     1141 agttttaaac agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg
     1201 gaattggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
     1261 tggggggagg ggtcggcaat tgatccggtg cctagagaag gtggcgcggg gtaaactggg
     1321 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
     1381 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
     1441 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
     1501 gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg
     1561 ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg
     1621 cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg
     1681 ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt
     1741 tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg
     1801 gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc
     1861 tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg
     1921 tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg
     1981 caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat
     2041 ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct
     2101 ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc
     2161 tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg
     2221 cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga
     2281 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc
     2341 agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgacgtacg gccaccatga
     2401 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     2461 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     2521 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     2581 gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt ctggaccacg ccggagagcg
     2641 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     2701 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     2761 cgtggttcct ggccaccgtc ggagtctcgc ccgaccacca gggcaagggt ctgggcagcg
     2821 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     2881 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     2941 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccggatcgg
     3001 gagagggcag aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct ggcccaccgg
     3061 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     3121 agctggacgg cgacgtaaac ggccacaagc tcagcgtgtc cggcgagggc gagggcgatg
     3181 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     3241 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     3301 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     3361 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     3421 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     3481 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     3541 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     3601 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     3661 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     3721 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     3781 acaagacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     3841 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     3901 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     3961 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4021 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4081 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4141 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     4201 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     4261 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     4321 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     4381 cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     4441 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     4501 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     4561 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     4621 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     4681 gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt atttataact
     4741 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     4801 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     4861 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     4921 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     4981 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     5041 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     5101 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     5161 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     5221 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     5281 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     5341 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     5401 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     5461 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     5521 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     5581 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     5641 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     5701 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     5761 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5821 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5881 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5941 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     6001 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     6061 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     6121 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     6181 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     6241 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     6301 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     6361 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     6421 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6481 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6541 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6601 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6661 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6721 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6781 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6841 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6901 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6961 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     7021 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     7081 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     7141 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     7201 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     7261 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     7321 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7381 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7441 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7501 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     7561 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     7621 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     7681 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     7741 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     7801 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     7861 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     7921 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
     7981 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
     8041 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
     8101 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
     8161 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8221 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8281 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     8341 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     8401 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     8461 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     8521 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     8581 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     8641 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     8701 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     8761 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     8821 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     8881 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     8941 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     9001 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     9061 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     9121 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     9181 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     9241 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     9301 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     9361 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     9421 tcgcaaaacc agcaagaa
//
