LOCUS       sequence-308788-        9408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of SARS-CoV-2 nsp12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Hillen HS, Kokic G, Farnung L, Dienemann C, Tegunov D, Cramer P
  TITLE     Structure of replicating SARS-CoV-2 polymerase.
  JOURNAL   Nature. 2020 May 21. pii: 10.1038/s41586-020-2368-8. doi: 
            10.1038/s41586-020-2368-8.
  PUBMED    32438371
REFERENCE   2  (bases 1 to 9408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2020 May 21. pii: 10.1038/s41586-020-2368-8. doi: 
            10.1038/s41586-020-2368-8."
COMMENT     Sequence Label: pFastBac-His-MBP-SCov2-nsp12
FEATURES             Location/Qualifiers
     source          1..9408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..19
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             139..156
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             166..1266
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1240..1263
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1321..1341
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     polyA_signal    4373..4507
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4410..4429)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4464..4483
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4581..4746
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4930..5384
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5017..5036)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5226..5247
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5411..5515
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5516..5584,5585..6376)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5734..5753)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6547..7135
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7036..7055
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7289..7306
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7387..7406)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7547..7569
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7563..7754)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     mobile_element  7979..8203
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(8270..8803)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(8973..8978,8979..8995,8996..9001))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa
       61 taaaaaaacc tataactatt ccggattatt cataccgtcc caccatcggg cgcggatccc
      121 ggaccgatgg gttcttctca ccatcaccat caccatggtt cttctatgaa aatcgaagaa
      181 ggtaaactgg taatctggat taacggcgat aaaggctata acggtctcgc tgaagtcggt
      241 aagaaattcg agaaagatac cggaattaaa gtcaccgttg agcatccgga taaactggaa
      301 gagaaattcc cacaggttgc ggcaactggc gatggccctg acattatctt ctgggcacac
      361 gaccgctttg gtggctacgc tcaatctggc ctgttggctg aaatcacccc ggacaaagcg
      421 ttccaggaca agctgtatcc gtttacctgg gatgccgtac gttacaacgg caagctgatt
      481 gcttacccga tcgctgttga agcgttatcg ctgatttata acaaagatct gctgccgaac
      541 ccgccaaaaa cctgggaaga gatcccggcg ctggataaag aactgaaagc gaaaggtaag
      601 agcgcgctga tgttcaacct gcaagaaccg tacttcacct ggccgctgat tgctgctgac
      661 gggggttatg cgttcaagta tgaaaacggc aagtacgaca ttaaagacgt gggcgtggat
      721 aacgctggcg cgaaagcggg tctgaccttc ctggttgacc tgattaaaaa caaacacatg
      781 aatgcagaca ccgattactc catcgcagaa gctgccttta ataaaggcga aacagcgatg
      841 accatcaacg gcccgtgggc atggtccaac atcgacacca gcaaagtgaa ttatggtgta
      901 acggtactgc cgaccttcaa gggtcaacca tccaaaccgt tcgttggcgt gctgagcgca
      961 ggtattaacg ccgccagtcc gaacaaagag ctggcaaaag agttcctcga aaactatctg
     1021 ctgactgatg aaggtctgga agcggttaat aaagacaaac cgctgggtgc cgtagcgctg
     1081 aagtcttacg aggaagagtt ggcgaaagat ccacgtattg ccgccactat ggaaaacgcc
     1141 cagaaaggtg aaatcatgcc gaacatcccg cagatgtccg ctttctggta tgccgtgcgt
     1201 actgcggtga tcaacgccgc cagcggtcgt cagactgtcg atgaagccct gaaagacgcg
     1261 cagactaatt cgagctcgaa caacaacaac aataacaata acaacaacct cgggatcgag
     1321 gaaaacctgt acttccaatc caatgcatct gctgacgctc agtccttcct gaaccgtgtg
     1381 tgcggtgtct ctgctgctcg tctgactcct tgcggtactg gaacctctac cgacgtggtg
     1441 taccgtgctt tcgacatcta caacgacaag gtggccggtt tcgctaagtt cctcaagacc
     1501 aactgctgcc gcttccaaga gaaggacgag gacgacaacc tgatcgactc ctacttcgtg
     1561 gtcaagcgtc acaccttctc caactaccag cacgaggaaa ctatctacaa cctgctgaag
     1621 gactgccccg ctgtggctaa gcacgacttc ttcaagttcc gtatcgacgg cgacatggtg
     1681 ccccacatct ctcgtcagcg tctgaccaag tacaccatgg ctgacctggt gtacgctctg
     1741 cgccacttcg atgagggaaa ctgcgacacc ctgaaagaga tcctggtcac ctacaactgc
     1801 tgtgacgacg actacttcaa caagaaggat tggtacgact tcgtcgagaa ccccgacatc
     1861 ctgcgtgtgt acgctaacct gggagagcgt gtgcgtcagg ctctgctcaa gactgtgcag
     1921 ttctgcgacg ctatgcgtaa cgctggtatc gtgggagtgc tgaccctgga caaccaggac
     1981 ctgaacggca attggtatga tttcggcgac ttcatccaga ctacccctgg ttctggtgtc
     2041 cccgtggtgg actcttacta ctccctgctg atgcccatcc tgactctgac ccgtgctctg
     2101 accgctgagt ctcacgtgga caccgacctg accaagcctt acatcaagtg ggacttgctg
     2161 aagtacgatt tcaccgagga acgcctgaag ctgttcgacc gttacttcaa gtactgggac
     2221 cagacctacc atcctaactg cgtgaactgc ctggacgacc gttgcatcct gcactgcgct
     2281 aacttcaacg tgctgttctc cactgtgttc cctccaacct ccttcggtcc tctcgtgcgc
     2341 aagatcttcg tggacggtgt ccctttcgtg gtgtccaccg gttaccactt ccgtgaactg
     2401 ggtgtcgtgc ataaccagga cgtcaacctc cactcctctc gtctgtcctt caaagagctg
     2461 ctggtctacg ctgctgaccc cgctatgcat gctgcttccg gaaacctgct gctggacaag
     2521 cgtaccactt gcttctccgt ggctgccctg actaacaacg tggccttcca gaccgtgaag
     2581 cccggcaatt tcaacaagga cttctacgat ttcgccgtgt ccaagggctt cttcaaagaa
     2641 ggctcctccg tcgagctgaa gcacttcttc ttcgctcaag acggcaacgc tgctatctcc
     2701 gactacgact actaccgtta caacttgccc accatgtgcg acatccgtca gctgctgttc
     2761 gtggtcgagg tggtggacaa gtacttcgac tgctacgacg gtggttgcat caacgccaat
     2821 caagtgatcg tgaacaacct ggacaagtcc gctggcttcc ccttcaacaa atggggcaaa
     2881 gctcgtctgt actacgactc catgtcctac gaggaccagg acgctctgtt cgcttacacc
     2941 aagcgtaacg tgatccccac catcactcag atgaacctga aatacgccat ctccgctaag
     3001 aaccgtgctc gtaccgtggc tggtgtctcc atctgctcca ccatgaccaa ccgtcagttc
     3061 caccagaagc tgctgaagtc aatcgctgct acccgcggtg ctaccgtggt catcggtact
     3121 tccaagttct acggtggctg gcacaacatg ctgaaaaccg tgtactccga cgtggaaaac
     3181 cctcacctga tgggctggga ctaccccaag tgcgatcgtg ctatgcctaa catgctgcgt
     3241 atcatggctt ccctggtgct ggctcgcaag cacactactt gctgctccct gtctcaccgt
     3301 ttctaccgtc tggctaacga gtgcgctcag gtgctgtccg agatggtcat gtgcggtggt
     3361 tccctgtacg tgaagcctgg tggtacttcc tctggcgacg ctaccaccgc ttacgctaac
     3421 tccgtgttca acatctgcca ggctgtgacc gctaacgtga acgctctgct gtccaccgac
     3481 ggcaacaaga tcgctgacaa atacgtgcgt aacctgcagc accgtctgta cgagtgcctg
     3541 taccgtaacc gtgacgtcga cactgacttc gtgaacgagt tctacgctta cctgcgcaag
     3601 cacttcagca tgatgatcct gtccgacgac gctgtcgtgt gcttcaactc cacctacgct
     3661 tcccaaggcc tggtggcttc catcaagaac ttcaagtccg tgctgtacta ccagaacaac
     3721 gtgttcatgt ccgaggctaa gtgctggacc gagactgacc tgactaaggg tccccacgag
     3781 ttctgctccc agcacaccat gctggtcaag cagggcgacg attacgtgta cctgccttat
     3841 cctgatcctt ctcgtatcct cggcgctggt tgcttcgtgg atgacatcgt caagaccgac
     3901 ggaaccctga tgatcgagcg tttcgtgtcc ctggctatcg acgcttaccc tctgactaag
     3961 caccccaatc aagagtacgc tgacgtgttc cacctgtacc tgcagtacat ccgcaagctg
     4021 cacgacgagc tgaccggaca catgctggac atgtactccg tgatgctgac caacgacaac
     4081 acctctcgtt actgggagcc cgaattctac gaggctatgt acacccctca caccgtgctg
     4141 caataataac attggaagtg gataacggat ccgaattcga gcgccgtcga caagcttgcg
     4201 gcctcatgat ctcctagggg ccgcactcga ggcatgcggt accaagcttg tcgagaagta
     4261 ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     4321 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     4381 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     4441 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     4501 ctggatctga tcactgcttg agcctagaaa catccaccat ttaaatggag ttaggtttaa
     4561 acgcctgcag gggagatccg aaccagataa gtgaaatcta gttccaaact attttgtcat
     4621 ttttaatttt cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt
     4681 ccctaaataa tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg
     4741 cccacagcgg ggcatttttc ttcctgttat gtttttaatc aaacatcctg ccaactccat
     4801 gtgacaaacc gtcatcttcg gctacttttt ctctgtcaca gaatgaaaat ttttctgtca
     4861 tctcttcgtt attaatgttt gtaattgact gaatatcaac gcttatttgc agcctgaatg
     4921 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4981 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     5041 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     5101 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     5161 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     5221 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     5281 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     5341 aaaatttaac gcgaatttta acaaactatt aacgtttaca atttcaggtg gcacttttcg
     5401 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5461 gctcatgaga caataaccct gataaatgct tcaatactat tgaaaaagga agagtatgag
     5521 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     5581 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     5641 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     5701 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     5761 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5821 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5881 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5941 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     6001 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     6061 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     6121 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     6181 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     6241 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     6301 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     6361 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     6421 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6481 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6541 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6601 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6661 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     6721 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6781 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6841 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6901 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6961 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     7021 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     7081 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     7141 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     7201 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     7261 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagaggc
     7321 gcgccatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc aatggtgcac
     7381 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     7441 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     7501 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     7561 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca
     7621 gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt
     7681 ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
     7741 tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata
     7801 ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta
     7861 ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca ggaccagccg
     7921 cgtaacctgg caaaatcggt tacggttgag taataaatgg atgccctgcg taagcgggtg
     7981 tgggcggaca ataaagtctt aaactgaaca aaatagatct aaactatgac aataaagtct
     8041 taaactagac agaatagttg taaactgaaa tcagtccagt tatgctgtga aaaagcatac
     8101 tggacttttg ttatggctaa agcaaactct tcattttctg aagtgcaaat tgcccgtcgt
     8161 attaaagagg ggcgtggcca agggcatggt aaagactata ttcgcggcgt tgtgacaatt
     8221 taccgaacaa ctccgcggcc gggaagccga tctcggcttg aacgaattgt taggtggcgg
     8281 tacttgggtc gatatcaaag tgcatcactt cttcccgtat gcccaacttt gtatagagag
     8341 ccactgcggg atcgtcaccg taatctgctt gcacgtagat cacataagca ccaagcgcgt
     8401 tggcctcatg cttgaggaga ttgatgagcg cggtggcaat gccctgcctc cggtgctcgc
     8461 cggagactgc gagatcatag atatagatct cactacgcgg ctgctcaaac ttgggcagaa
     8521 cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg
     8581 tcttactacg gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg
     8641 ctacgtctcc gaactcacga ccgaaaagat caagagcagc ccgcatggat ttgacttggt
     8701 cagggccgag cctacatgtg cgaatgatgc ccatacttga gccacctaac tttgttttag
     8761 ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg
     8821 cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac
     8881 aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc
     8941 agttgcgtga gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca
     9001 actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca
     9061 gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg caaggtttcg gtctccacgc
     9121 atcgtcaggc attggcggcc ttgctgttct tctacggcaa ggtgctgtgc acggatctgc
     9181 cctggcttca ggagatcgga agacctcggc cgtcgcggcg cttgccggtg gtgctgaccc
     9241 cggatgaagt ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg ttcgcccagg
     9301 actctagcta tagttctagt ggttggctac gtatactccg gactattaat agatcatgga
     9361 gataattagc ggccgcgttt aaacatccac catttgatgg agataatt
//
