LOCUS       sequence-308801-        9193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MBP-Axin-His (human Axin as a fusion protein with MBP and 
            His- tags) in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9193)
  AUTHORS   Gavagan M, Fagnan E, Speltz EB, Zalatan JG
  TITLE     The Scaffold Protein Axin Promotes Signaling Specificity within the 
            Wnt Pathway by Suppressing Competing Kinase Reactions.
  JOURNAL   Cell Syst. 2020 Jun 24;10(6):515-525.e5. doi: 
            10.1016/j.cels.2020.05.002. Epub 2020 Jun 17.
  PUBMED    32553184
REFERENCE   2  (bases 1 to 9193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2020.05.002"; journalName: "Cell Syst"; date: 
            "2020-06-24- 24"; volume: "10"; issue: "6"; pages: "515-525.e5"
COMMENT     Sequence Label: pEF073 MBP-Axin-His
FEATURES             Location/Qualifiers
     source          1..9193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..78
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             79..1161
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(98..117)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(1404..1409,1410..1425,1426..1432)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1440..1456
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1445..1467
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             1526..2626
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      2483..2492
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     2600..2623
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2675..2686
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2729..2749
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5240..5257
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5284..5301)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5284..5300)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5293..5315)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5479..5496)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      5649..5735
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5827..5854
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        5873..5964
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5965..6033,6034..6825)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6183..6202)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(6867..7380)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7062..7083)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7274..7293
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      7491..8079
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7980..7999
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8233..8250
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8265..8405
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8331..8350)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8491..8513
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8507..8698)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 gacaccatcg aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt
       61 caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt
      121 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg
      181 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa
      241 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac
      301 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg
      361 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt
      421 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt
      481 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca
      541 cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg
      601 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg
      661 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg
      721 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat
      781 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg
      841 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac
      901 gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc
      961 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag
     1021 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg
     1081 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     1141 cgactggaaa gcgggcagtg agcgcaacgc aattaatgta agttagctca ctcattaggc
     1201 acaattctca tgtttgacag cttatcatcg actgcacggt gcaccaatgc ttctggcgtc
     1261 aggcagccat cggaagctgt ggtatggctg tgcaggtcgt aaatcactgc ataattcgtg
     1321 tcgctcaagg cgcactcccg ttctggataa tgttttttgc gccgacatca taacggttct
     1381 ggcaaatatt ctgaaatgag ctgttgacaa ttaatcatcg gctcgtataa tgtgtggaat
     1441 tgtgagcgga taacaatttc acacaggaaa cagccagtcc gtttaggtgt tttcacgagc
     1501 acttcaccaa caaggaccat agattatgaa aactgaagaa ggtaaactgg taatctggat
     1561 taacggcgat aaaggctata acggtctcgc tgaagtcggt aagaaattcg agaaagatac
     1621 cggaattaaa gtcaccgttg agcatccgga taaactggaa gagaaattcc cacaggttgc
     1681 ggcaactggc gatggccctg acattatctt ctgggcacac gaccgctttg gtggctacgc
     1741 tcaatctggc ctgttggctg aaatcacccc ggacaaagcg ttccaggaca agctgtatcc
     1801 gtttacctgg gatgccgtac gttacaacgg caagctgatt gcttacccga tcgctgttga
     1861 agcgttatcg ctgatttata acaaagatct gctgccgaac ccgccaaaaa cctgggaaga
     1921 gatcccggcg ctggataaag aactgaaagc gaaaggtaag agcgcgctga tgttcaacct
     1981 gcaagaaccg tacttcacct ggccgctgat tgctgctgac gggggttatg cgttcaagta
     2041 tgaaaacggc aagtacgaca ttaaagacgt gggcgtggat aacgctggcg cgaaagcggg
     2101 tctgaccttc ctggttgacc tgattaaaaa caaacacatg aatgcagaca ccgattactc
     2161 catcgcagaa gctgccttta ataaaggcga aacagcgatg accatcaacg gcccgtgggc
     2221 atggtccaac atcgacacca gcaaagtgaa ttatggtgta acggtactgc cgaccttcaa
     2281 gggtcaacca tccaaaccgt tcgttggcgt gctgagcgca ggtattaacg ccgccagtcc
     2341 gaacaaagag ctggcaaaag agttcctcga aaactatctg ctgactgatg aaggtctgga
     2401 agcggttaat aaagacaaac cgctgggtgc cgtagcgctg aagtcttacg aggaagagtt
     2461 ggcgaaagat ccacgtattg ccgccaccat ggaaaacgcc cagaaaggtg aaatcatgcc
     2521 gaacatcccg cagatgtccg ctttctggta tgccgtgcgt actgcggtga tcaacgccgc
     2581 cagcggtcgt cagactgtcg atgaagccct gaaagacgcg cagactaatt cgagctcgaa
     2641 caacaacaac aataacaata acaacaacct cgggatcgag ggaaggattt caactagtgg
     2701 atctggtggt ggtggcggtt ccatgagcga gaatctttat tttcagggat ccatgaatat
     2761 ccaagagcag ggtttcccct tggacctcgg agcaagtttc accgaagatg ctccccgacc
     2821 cccagtgcct ggtgaggagg gagaactggt gtccacagac ccgaggcccg ccagctacag
     2881 tttctgctcc gggaaaggtg ttggcattaa aggtgagact tcgacggcca ctccgaggcg
     2941 ctcggatctg gacctggggt atgagcctga gggcagtgcc tcccccaccc caccatactt
     3001 gaagtgggct gagtcactgc attccctgct ggatgaccaa gatgggataa gcctgttcag
     3061 gactttcctg aagcaggagg gctgtgccga cttgctggac ttctggtttg cctgcactgg
     3121 cttcaggaag ctggagccct gtgactcgaa cgaggagaag aggctgaagc tggcgagagc
     3181 catctaccga aagtacattc ttgataacaa tggcatcgtg tcccggcaga ccaagccagc
     3241 caccaagagc ttcataaagg gctgcatcat gaagcagctg atcgatcctg ccatgtttga
     3301 ccaggcccag accgaaatcc aggccactat ggaggaaaac acctatccct ccttccttaa
     3361 gtctgatatt tatttggaat atacgaggac aggctcggag agccccaaag tctgtagtga
     3421 ccagagctct gggtcaggga cagggaaggg catatctgga tacctgccga ccttaaatga
     3481 agatgaggaa tggaagtgtg accaggacat ggatgaggac gatggcagag acgctgctcc
     3541 ccccggaaga ctccctcaga agctgctcct ggagacagct gccccgaggg tctcctccag
     3601 tagacggtac agcgaaggca gagagttcag gtatggatcc tggcgggagc cagtcaaccc
     3661 ctattatgtc aatgccggct atgccctggc cccagccacc agtgccaacg acagcgagca
     3721 gcagagcctg tccagcgatg cagacaccct gtccctcacg gacagcagcg tggatgggat
     3781 ccccccatac aggatccgta agcagcaccg cagggagatg caggagagcg tgcaggtcaa
     3841 tgggcgggtg cccctacctc acattccccg cacgtaccgg gtgccgaagg aggtccgcgt
     3901 ggagcctcag aagttcgcgg aggagctcat ccaccgcctg gaggctgtgc agcgcacgcg
     3961 ggaggccgag gagaagctgg aggagcggct gaagcgcgtg cgcatggagg aggaaggtga
     4021 ggacggcgat ccatcgtcag ggcccccagg gccgtgtcac aagctgcctc ccgcccccgc
     4081 ttggcaccac ttcccgcccc gctgtgtgga catgggctgt gccgggctcc gggatgcaca
     4141 cgaggagaac cctgagagca tcctggacga gcacgtacag cgtgtgctga ggacacctgg
     4201 ccgccagtcg cctgggcctg gccatcgctc cccggacagt gggcacgtgg ccaagatgcc
     4261 agtggcactg gggggtgccg cctcggggca cgggaagcac gtacccaagt caggggcgaa
     4321 gctggacgcg gccggcctgc accaccaccg acacgtccac caccacgtcc accacagcac
     4381 agcccggccc aaggagcagg tggaggccga ggccacccgc agggcccaga gcagcttcgc
     4441 ctggggcctg gaaccacaca gccatggggc aaggtcccga ggctactcag agagtgttgg
     4501 cgctgccccc aacgccagtg atggcctcgc ccacagtggg aaggtgggcg tggcgtgcaa
     4561 aagaaatgcc aagaaggctg agtcggggaa gagcgccagc accgaggtgc caggtgcctc
     4621 ggaggatgcg gagaagaacc agaaaatcat gcagtggatc attgaggggg aaaaggagat
     4681 cagcaggcac cgcaggaccg gccacgggtc ttcggggacg aggaagccac agccccatga
     4741 gaactccaga cccttgtccc ttgagcaccc ctgggccggc cctcagctcc ggacctccgt
     4801 gcagccctcc cacctcttca tccaagaccc caccatgcca ccccacccag ctcccaaccc
     4861 cctaacccag ctggaggagg cgcgccgacg tctggaggag gaagaaaaga gagccagccg
     4921 agcaccctcc aagcagagga caagatcgca gaggaaggtg ggcggcggga gtgcccagcc
     4981 gtgtgacagc atcgttgtgg cgtactactt ctgcggggaa cccatcccct accgcaccct
     5041 ggtgaggggc cgcgctgtca ccctgggcca gttcaaggag ctgctgacca aaaagggcag
     5101 ctacagatac tacttcaaga aagtgagcga cgagtttgac tgtggggtgg tgtttgagga
     5161 ggttcgagag gacgaggccg tcctgcccgt ctttgaggag aagatcatcg gcaaagtgga
     5221 gaaggtggac agcggccgcc accatcacca ccaccactag gggccgcact cgagaagctt
     5281 ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     5341 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     5401 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cagcttggct gttttggcgg
     5461 atgagataag attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa
     5521 acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga
     5581 agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg
     5641 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     5701 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
     5761 cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa
     5821 ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact cttttgttta
     5881 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5941 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     6001 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     6061 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     6121 aagatccttg agagttttcg ccccgaagaa cgtttcccaa tgatgagcac ttttaaagtt
     6181 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     6241 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     6301 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     6361 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     6421 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     6481 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     6541 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     6601 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     6661 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     6721 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     6781 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     6841 tactcatata tactttagat tgatttaccc cggttgataa tcagaaaagc cccaaaaaca
     6901 ggaagattgt ataagcaaat atttaaattg taaacgttaa tattttgtta aaattcgcgt
     6961 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     7021 ataaatcaaa agaatagccc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     7081 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     7141 gcccactacg tgaaccatca cccaaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     7201 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     7261 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     7321 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     7381 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     7441 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     7501 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7561 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7621 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     7681 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7741 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7801 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7861 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7921 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7981 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     8041 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     8101 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     8161 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     8221 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     8281 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     8341 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     8401 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     8461 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     8521 cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgcagcg
     8581 attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta
     8641 atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg
     8701 atgcctccgt gtaatgggga tttctgttca tgggggtaat gataccgatg aaacgagaga
     8761 ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg
     8821 gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc
     8881 agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc
     8941 agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac
     9001 ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc
     9061 ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc
     9121 ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc
     9181 tgcccgaaat tcc
//
