LOCUS       sequence-308804-        9031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral overexpression of WT METTL14 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9031)
  AUTHORS   Weng H, Huang H, Wu H, Qin X, Zhao BS, Dong L, Shi H, Skibbe J, Shen
            C, Hu C, Sheng Y, Wang Y, Wunderlich M, Zhang B, Dore LC, Su R, Deng
            X, Ferchen K, Li C, Sun M, Lu Z, Jiang X, Marcucci G, Mulloy JC, 
            Yang J, Qian Z, Wei M, He C, Chen J
  TITLE     METTL14 Inhibits Hematopoietic Stem/Progenitor Differentiation and 
            Promotes Leukemogenesis via mRNA m(6)A Modification.
  JOURNAL   Cell Stem Cell. 2018 Feb 1;22(2):191-205.e9. doi: 
            10.1016/j.stem.2017.11.016. Epub 2017 Dec 28.
  PUBMED    29290617
REFERENCE   2  (bases 1 to 9031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2017.11.016"; journalName: "Cell Stem Cell"; date: 
            "2018-02-1- 1"; volume: "22"; issue: "2"; pages: "191-205.e9"
COMMENT     Sequence Label: MSCV-PIG-METTL14-WT
FEATURES             Location/Qualifiers
     source          1..9031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             91..607
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    671..1012
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1079..1495
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1425..1447
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1463..1479
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1523..1549
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        2934..3433
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2969..2992)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3366..3385
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3454..4053
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3454..3473)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3950..3970
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4114..4681
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4131..4683
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4275..4292)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4502..4521
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4695..4697,4698..4700,4701..5414)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4740..4761)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5001..5020)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5348..5369
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5516..6030
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6192..6208
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6216..6222,6223..6240,6241..6246))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6261..6282
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6399..6416)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6570..7158)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6650..6669)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7329..8120,8121..8189))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7952..7971
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8190..8294)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8362..8380
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8418..8440)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8540..8559
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8753..8775
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8903..8922
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tttgactcaa caatatcacc agctgaagcc tatagagtac gagccataga taaaataaaa
       61 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
      121 gctagcttaa gtaacgccat tttgcaaggc atggaaaata cataactgag aatagagaag
      181 ttcagatcaa ggttaggaac agagagacag cagaatatgg gccaaacagg atatctgtgg
      241 taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtcccgcc
      301 ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac
      361 cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg
      421 ctccccgagc tcaataaaag agcccacaac ccctcactcg gcgcgccagt cctccgatag
      481 actgcgtcgc ccgggtaccc gtattcccaa taaagcctct tgctgtttgc atccgaatcg
      541 tggactcgct gatccttggg agggtctcct cagattgatt gactgcccac ctcgggggtc
      601 tttcatttgg aggttccacc gagatttgga gacccctgcc cagggaccac cgaccccccc
      661 gccgggaggt aagctggcca gcggtcgttt cgtgtctgtc tctgtctttg tgcgtgtttg
      721 tgccggcatc taatgtttgc gcctgcgtct gtactagtta gctaactagc tctgtatctg
      781 gcggacccgt ggtggaactg acgagttctg aacacccggc cgcaaccctg ggagacgtcc
      841 cagggacttt gggggccgtt tttgtggccc gacctgagga agggagtcga tgtggaatcc
      901 gaccccgtca ggatatgtgg ttctggtagg agacgagaac ctaaaacagt tcccgcctcc
      961 gtctgaattt ttgctttcgg tttggaaccg aagccgcgcg tcttgtctgc tgcagcgctg
     1021 cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg aaaattaggg
     1081 ccagactgtt accactccct taagtttgac cttaggtcac tggaaagatg tcgagcggat
     1141 cgctcacaac cagtcggtag atgtcaagaa gagacgttgg gttaccttct gctctgcaga
     1201 atggccaacc tttaacgtcg gatggccgcg agacggcacc tttaaccgag acctcatcac
     1261 ccaggttaag atcaaggtct tttcacctgg cccgcatgga cacccagacc aggtccccta
     1321 catcgtgacc tgggaagcct tggcttttga cccccctccc tgggtcaagc cctttgtaca
     1381 ccctaagcct ccgcctcctc ttcctccatc cgccccgtct ctcccccttg aacctcctcg
     1441 ttcgaccccg cctcgatcct ccctttatcc agccctcact ccttctctag gcgccggaat
     1501 tagatctctc gagaccacca tgtacccata cgatgttcca gattacgctg atagccgctt
     1561 gcaggagatc cgggagcggc agaagttacg gcgacagctc ctcgcgcagc agttgggagc
     1621 tgaaagtgcc gacagcattg gtgccgtgtt aaatagcaaa gatgagcaga gagaaattgc
     1681 tgaaacaaga gaaacttgca gggcttccta tgatacctct gctccaaatg caaaacgtaa
     1741 gtatctggat gaaggagaga cagatgaaga caaaatggaa gaatataagg atgaactaga
     1801 aatgcaacag gatgaagaaa atttgccata tgaagaagag atttacaaag attctagtac
     1861 ttttcttaag ggaacacaga gcttaaatcc ccataatgat tactgccaac attttgtaga
     1921 cactggacat agacctcaga atttcatcag ggatgtaggt ttagctgaca gatttgaaga
     1981 atatcctaaa ctgagggagc tcatcaggct aaaggatgag ttaatagcta aatctaacac
     2041 tcctcccatg tacttacaag ccgatataga agcctttgac atcagagaac taacacccaa
     2101 atttgatgtg attcttctgg aacccccttt agaagaatat tacagagaaa ctggcatcac
     2161 tgctaatgaa aaatgctgga cttgggatga tattatgaag ttagaaattg atgagattgc
     2221 agcacctcga tcatttattt ttctctggtg tggttctggg gaggggttgg accttggaag
     2281 agtgtgttta cgaaaatggg gttacagaag atgtgaagat atttgttgga ttaaaaccaa
     2341 taaaaacaat cctgggaaga ctaagacttt agatccaaag gctgtctttc agagaacaaa
     2401 ggaacactgc ctcatgggga tcaaaggaac tgtgaagcgt agcacagacg gggacttcat
     2461 tcatgctaat gttgacattg acttaattat cacagaagaa cctgaaattg gcaatataga
     2521 aaaacctgta gaaatttttc atataattga gcatttttgt cttggtagaa gacgccttca
     2581 tctatttgga agagatagta caattcgacc aggctggctc acagttggac caacgcttac
     2641 aaatagcaac tacaatgcag aaacatatgc atcctatttc agtgctccta attcctactt
     2701 gactggttgt acagaagaaa ttgagagact tcgaccaaaa tcgcctcctc ccaaatctaa
     2761 atctgaccga ggaggtggag ctcccagagg tggaggaaga ggtggaactt ctgctggccg
     2821 tggacgagaa agaaatagat ctaacttccg aggagaaaga ggtggcttta gagggggccg
     2881 tggaggagca cacagaggtg gctttccacc tcgataagtt aacgaattct accgggtagg
     2941 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg
     3001 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc
     3061 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
     3121 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
     3181 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
     3241 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
     3301 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
     3361 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
     3421 tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc
     3481 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     3541 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     3601 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     3661 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     3721 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     3781 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     3841 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     3901 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     3961 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     4021 acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg
     4081 accgaaagga gcgcacgacc ccatgcatcc aattcccgcc ccccccccta acgttactgg
     4141 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     4201 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     4261 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     4321 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     4381 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     4441 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     4501 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     4561 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     4621 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     4681 atatggccac aaccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     4741 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtctggc gagggcgagg
     4801 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     4861 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     4921 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     4981 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     5041 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     5101 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     5161 acaagcagaa gaacggcatc aaggcgaact tcaagatccg ccacaacatc gaggacggca
     5221 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     5281 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     5341 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     5401 agctgtacaa gtaatgaatt aattaagaat tatcaagctt atcgataccg tcgacctgca
     5461 gccaagctta tcgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa
     5521 agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa
     5581 aatacataac tgagaataga gaagttcaga tcaaggttag gaacagagag acagcagaat
     5641 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
     5701 atggtcccca gatgcggtcc cgccctcagc agtttctaga gaaccatcag atgtttccag
     5761 ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt
     5821 ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca
     5881 ctcggcgcgc cagtcctccg atagactgcg tcgcccgggt acccgtgtat ccaataaacc
     5941 ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg
     6001 attgactacc cgtcagcggg ggtctttcat gggtaacagt ttcttgaagt tggagaacaa
     6061 cattctgagg gtaggagtcg aatattaagt aatcctgact caattagcca ctgttttgaa
     6121 tccacatact ccaatactcc tgaaatagtt cattatggac agcgcagaaa gagctgggga
     6181 gaattgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6241 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6301 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6361 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6421 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6481 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6541 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6601 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6661 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6721 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     6781 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6841 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     6901 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6961 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7021 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7081 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7141 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7201 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7261 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7321 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7381 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7441 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7501 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7561 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7621 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7681 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7741 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7801 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7861 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     7921 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     7981 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8041 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8101 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8161 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8221 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8281 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     8341 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
     8401 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     8461 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     8521 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     8581 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
     8641 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     8701 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     8761 cgacgttgta aaacgacggc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
     8821 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
     8881 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
     8941 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gcgattagtc caatttgtta
     9001 aagacaggat atcagtggtc caggctctag t
//
