LOCUS       sequence-308805-        9058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MBP-beta-catenin-His (human beta-catenin as a fusion 
            protein with MBP and His- tags) in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Gavagan M, Fagnan E, Speltz EB, Zalatan JG
  TITLE     The Scaffold Protein Axin Promotes Signaling Specificity within the 
            Wnt Pathway by Suppressing Competing Kinase Reactions.
  JOURNAL   Cell Syst. 2020 Jun 24;10(6):515-525.e5. doi: 
            10.1016/j.cels.2020.05.002. Epub 2020 Jun 17.
  PUBMED    32553184
REFERENCE   2  (bases 1 to 9058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2020.05.002"; journalName: "Cell Syst"; date: 
            "2020-06-24- 24"; volume: "10"; issue: "6"; pages: "515-525.e5"
COMMENT     Sequence Label: pEF019 MBP-beta-catenin-His
FEATURES             Location/Qualifiers
     source          1..9058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(720..725,726..741,742..748)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    756..772
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     761..783
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             842..1942
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1799..1808
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1916..1939
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1991..2002
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2045..2065
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2069..4411
                     /codon_start=1
                     /gene="CTNNB1"
                     /product="protein involved both in cell-cell adhesion as 
                     part of the cadherin protein complex, and in gene 
                     transcription as part of the Wnt signaling pathway"
                     /label=CTNNB1
                     /note="also known as catenin beta-1; related to Drosophila 
                     armadillo"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     CDS             2069..4411
                     /codon_start=1
                     /gene="Homo sapiens CTNNB1"
                     /product="human cadherin-associated protein that also plays
                     a role in the canonical Wnt signaling pathway"
                     /label=beta-catenin
                     /note="also known as catenin beta-1"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     CDS             4421..4438
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4465..4482)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4465..4481)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4474..4496)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4660..4677)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      4830..4916
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5008..5035
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        5054..5145
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5146..5214,5215..6006)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5364..5383)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(6048..6561)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6243..6264)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6455..6474
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      6672..7260
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7161..7180
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7414..7431
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7446..7586
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7512..7531)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7672..7694
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7688..7879)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        8375..8452
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             join(8453..9058,1..477)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(8472..8491)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc
       61 atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg
      121 ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg
      181 cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat
      241 atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac
      301 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca
      361 ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
      421 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
      481 caacgcaatt aatgtaagtt agctcactca ttaggcacaa ttctcatgtt tgacagctta
      541 tcatcgactg cacggtgcac caatgcttct ggcgtcaggc agccatcgga agctgtggta
      601 tggctgtgca ggtcgtaaat cactgcataa ttcgtgtcgc tcaaggcgca ctcccgttct
      661 ggataatgtt ttttgcgccg acatcataac ggttctggca aatattctga aatgagctgt
      721 tgacaattaa tcatcggctc gtataatgtg tggaattgtg agcggataac aatttcacac
      781 aggaaacagc cagtccgttt aggtgttttc acgagcactt caccaacaag gaccatagat
      841 tatgaaaact gaagaaggta aactggtaat ctggattaac ggcgataaag gctataacgg
      901 tctcgctgaa gtcggtaaga aattcgagaa agataccgga attaaagtca ccgttgagca
      961 tccggataaa ctggaagaga aattcccaca ggttgcggca actggcgatg gccctgacat
     1021 tatcttctgg gcacacgacc gctttggtgg ctacgctcaa tctggcctgt tggctgaaat
     1081 caccccggac aaagcgttcc aggacaagct gtatccgttt acctgggatg ccgtacgtta
     1141 caacggcaag ctgattgctt acccgatcgc tgttgaagcg ttatcgctga tttataacaa
     1201 agatctgctg ccgaacccgc caaaaacctg ggaagagatc ccggcgctgg ataaagaact
     1261 gaaagcgaaa ggtaagagcg cgctgatgtt caacctgcaa gaaccgtact tcacctggcc
     1321 gctgattgct gctgacgggg gttatgcgtt caagtatgaa aacggcaagt acgacattaa
     1381 agacgtgggc gtggataacg ctggcgcgaa agcgggtctg accttcctgg ttgacctgat
     1441 taaaaacaaa cacatgaatg cagacaccga ttactccatc gcagaagctg cctttaataa
     1501 aggcgaaaca gcgatgacca tcaacggccc gtgggcatgg tccaacatcg acaccagcaa
     1561 agtgaattat ggtgtaacgg tactgccgac cttcaagggt caaccatcca aaccgttcgt
     1621 tggcgtgctg agcgcaggta ttaacgccgc cagtccgaac aaagagctgg caaaagagtt
     1681 cctcgaaaac tatctgctga ctgatgaagg tctggaagcg gttaataaag acaaaccgct
     1741 gggtgccgta gcgctgaagt cttacgagga agagttggcg aaagatccac gtattgccgc
     1801 caccatggaa aacgcccaga aaggtgaaat catgccgaac atcccgcaga tgtccgcttt
     1861 ctggtatgcc gtgcgtactg cggtgatcaa cgccgccagc ggtcgtcaga ctgtcgatga
     1921 agccctgaaa gacgcgcaga ctaattcgag ctcgaacaac aacaacaata acaataacaa
     1981 caacctcggg atcgagggaa ggatttcaac tagtggatct ggtggtggtg gcggttccat
     2041 gagcgagaat ctttattttc agggatccat ggctactcaa gctgatttga tggagttgga
     2101 catggccatg gaaccagaca gaaaagcggc tgttagtcac tggcagcaac agtcttacct
     2161 ggactctgga atccattctg gtgccactac cacagctcct tctctgagtg gtaaaggcaa
     2221 tcctgaggaa gaggatgtgg atacctccca agtcctgtat gagtgggaac agggattttc
     2281 tcagtccttc actcaagaac aagtagctga tattgatgga cagtatgcaa tgactcgagc
     2341 tcagagggta cgagctgcta tgttccctga gacattagat gagggcatgc agatcccatc
     2401 tacacagttt gatgctgctc atcccactaa tgtccagcgt ttggctgaac catcacagat
     2461 gctgaaacat gcagttgtaa acttgattaa ctatcaagat gatgcagaac ttgccacacg
     2521 tgcaatccct gaactgacaa aactgctaaa tgacgaggac caggtggtgg ttaataaggc
     2581 tgcagttatg gtccatcagc tttctaaaaa ggaagcttcc agacacgcta tcatgcgttc
     2641 tcctcagatg gtgtctgcta ttgtacgtac catgcagaat acaaatgatg tagaaacagc
     2701 tcgttgtacc gctgggacct tgcataacct ttcccatcat cgtgagggct tactggccat
     2761 ctttaagtct ggaggcattc ctgccctggt gaaaatgctt ggttcaccag tggattctgt
     2821 gttgttttat gccattacaa ctctccacaa ccttttatta catcaagaag gagctaaaat
     2881 ggcagtgcgt ttagctggtg ggctgcagaa aatggttgcc ttgctcaaca aaacaaatgt
     2941 taaattcttg gctattacga cagactgcct tcaaatttta gcttatggca accaagaaag
     3001 caagctcatc atactggcta gtggtggacc ccaagcttta gtaaatataa tgaggaccta
     3061 tacttacgaa aaactactgt ggaccacaag cagagtgctg aaggtgctat ctgtctgctc
     3121 tagtaataag ccggctattg tagaagctgg tggaatgcaa gctttaggac ttcacctgac
     3181 agatccaagt caacgtcttg ttcagaactg tctttggact ctcaggaatc tttcagatgc
     3241 tgcaactaaa caggaaggga tggaaggtct ccttgggact cttgttcagc ttctgggttc
     3301 agatgatata aatgtggtca cctgtgcagc tggaattctt tctaacctca cttgcaataa
     3361 ttataagaac aagatgatgg tctgccaagt gggtggtata gaggctcttg tgcgtactgt
     3421 ccttcgggct ggtgacaggg aagacatcac tgagcctgcc atctgtgctc ttcgtcatct
     3481 gaccagccga caccaagaag cagagatggc ccagaatgca gttcgccttc actatggact
     3541 accagttgtg gttaagctct tacacccacc atcccactgg cctctgataa aggctactgt
     3601 tggattgatt cgaaatcttg ccctttgtcc cgcaaatcat gcacctttgc gtgagcaggg
     3661 tgccattcca cgactagttc agttgcttgt tcgtgcacat caggataccc agcgccgtac
     3721 gtccatgggt gggacacagc agcaatttgt ggagggggtc cgcatggaag aaatagttga
     3781 aggttgtacc ggagcccttc acatcctagc tcgggatgtt cacaaccgaa ttgttatcag
     3841 aggactaaat accattccat tgtttgtgca gctgctttat tctcccattg aaaacatcca
     3901 aagagtagct gcaggggtcc tctgtgaact tgctcaggac aaggaagctg cagaagctat
     3961 tgaagctgag ggagccacag ctcctctgac agagttactt cactctagga atgaaggtgt
     4021 ggcgacatat gcagctgctg ttttgttccg aatgtctgag gacaagccac aagattacaa
     4081 gaaacggctt tcagttgagc tgaccagctc tctcttcaga acagagccaa tggcttggaa
     4141 tgagactgct gatcttggac ttgatattgg tgcccaggga gaaccccttg gatatcgcca
     4201 ggatgatcct agctatcgtt cttttcactc tggtggatat ggccaggatg ccttgggtat
     4261 ggaccccatg atggaacatg agatgggtgg ccaccaccct ggtgctgact atccagttga
     4321 tgggctgcca gatctggggc atgcccagga cctcatggat gggctgcctc caggtgacag
     4381 caatcagctg gcctggtttg atactgacct gagcggccgc caccatcacc accaccacta
     4441 ggggccgcac tcgagaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa
     4501 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     4561 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     4621 gcagcttggc tgttttggcg gatgagataa gattttcagc ctgatacaga ttaaatcaga
     4681 acgcagaagc ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc
     4741 tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc
     4801 ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     4861 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
     4921 cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc
     4981 cataaactgc caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt
     5041 ttctacaaac tcttttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5101 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5161 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5221 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5281 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgtttccca
     5341 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     5401 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5461 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5521 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5581 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5641 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     5701 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5761 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     5821 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     5881 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     5941 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6001 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttacc ccggttgata
     6061 atcagaaaag ccccaaaaac aggaagattg tataagcaaa tatttaaatt gtaaacgtta
     6121 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     6181 ccgaaatcgg caaaatccct tataaatcaa aagaatagcc cgagataggg ttgagtgttg
     6241 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     6301 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc acccaaatca agttttttgg
     6361 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     6421 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     6481 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     6541 atgcgccgct acagggcgcg taaaaggatc taggtgaaga tcctttttga taatctcatg
     6601 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6661 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6721 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6781 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     6841 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6901 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6961 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     7021 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     7081 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     7141 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     7201 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     7261 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7321 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     7381 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     7441 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     7501 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     7561 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     7621 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     7681 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
     7741 catcagcgtg gtcgtgcagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
     7801 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
     7861 ttttttcctg tttggtcact gatgcctccg tgtaatgggg atttctgttc atgggggtaa
     7921 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc
     7981 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa
     8041 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta
     8101 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg
     8161 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag
     8221 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac
     8281 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca
     8341 cccgtggcca ggacccaacg ctgcccgaaa ttccgacacc atcgaatggt gcaaaacctt
     8401 tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc
     8461 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
     8521 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
     8581 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
     8641 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
     8701 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
     8761 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
     8821 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
     8881 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
     8941 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
     9001 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcat
//
