LOCUS       sequence-308809-        9031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MBP-Axin∆BCD-His (human Axin with aa465-518 deletion as a 
            fusion protein with MBP and His- tags) in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9031)
  AUTHORS   Gavagan M, Fagnan E, Speltz EB, Zalatan JG
  TITLE     The Scaffold Protein Axin Promotes Signaling Specificity within the 
            Wnt Pathway by Suppressing Competing Kinase Reactions.
  JOURNAL   Cell Syst. 2020 Jun 24;10(6):515-525.e5. doi: 
            10.1016/j.cels.2020.05.002. Epub 2020 Jun 17.
  PUBMED    32553184
REFERENCE   2  (bases 1 to 9031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2020.05.002"; journalName: "Cell Syst"; date: 
            "2020-06-24- 24"; volume: "10"; issue: "6"; pages: "515-525.e5"
COMMENT     Sequence Label: pMG035 MBP-Axin∆BCD-His
FEATURES             Location/Qualifiers
     source          1..9031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..78
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             79..1161
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(98..117)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(1404..1409,1410..1425,1426..1432)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1440..1456
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1445..1467
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             1526..2626
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      2483..2492
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     2600..2623
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2675..2686
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2729..2749
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5078..5095
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5122..5139)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5122..5138)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5131..5153)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5317..5334)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      5487..5573
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5665..5692
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        5711..5802
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5803..5871,5872..6663)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6021..6040)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(6705..7218)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6900..6921)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7112..7131
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      7329..7917
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7818..7837
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8071..8088
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8103..8243
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8169..8188)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8329..8351
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8345..8536)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 gacaccatcg aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt
       61 caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt
      121 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg
      181 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa
      241 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac
      301 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg
      361 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt
      421 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt
      481 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca
      541 cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg
      601 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg
      661 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg
      721 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat
      781 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg
      841 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac
      901 gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc
      961 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag
     1021 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg
     1081 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     1141 cgactggaaa gcgggcagtg agcgcaacgc aattaatgta agttagctca ctcattaggc
     1201 acaattctca tgtttgacag cttatcatcg actgcacggt gcaccaatgc ttctggcgtc
     1261 aggcagccat cggaagctgt ggtatggctg tgcaggtcgt aaatcactgc ataattcgtg
     1321 tcgctcaagg cgcactcccg ttctggataa tgttttttgc gccgacatca taacggttct
     1381 ggcaaatatt ctgaaatgag ctgttgacaa ttaatcatcg gctcgtataa tgtgtggaat
     1441 tgtgagcgga taacaatttc acacaggaaa cagccagtcc gtttaggtgt tttcacgagc
     1501 acttcaccaa caaggaccat agattatgaa aactgaagaa ggtaaactgg taatctggat
     1561 taacggcgat aaaggctata acggtctcgc tgaagtcggt aagaaattcg agaaagatac
     1621 cggaattaaa gtcaccgttg agcatccgga taaactggaa gagaaattcc cacaggttgc
     1681 ggcaactggc gatggccctg acattatctt ctgggcacac gaccgctttg gtggctacgc
     1741 tcaatctggc ctgttggctg aaatcacccc ggacaaagcg ttccaggaca agctgtatcc
     1801 gtttacctgg gatgccgtac gttacaacgg caagctgatt gcttacccga tcgctgttga
     1861 agcgttatcg ctgatttata acaaagatct gctgccgaac ccgccaaaaa cctgggaaga
     1921 gatcccggcg ctggataaag aactgaaagc gaaaggtaag agcgcgctga tgttcaacct
     1981 gcaagaaccg tacttcacct ggccgctgat tgctgctgac gggggttatg cgttcaagta
     2041 tgaaaacggc aagtacgaca ttaaagacgt gggcgtggat aacgctggcg cgaaagcggg
     2101 tctgaccttc ctggttgacc tgattaaaaa caaacacatg aatgcagaca ccgattactc
     2161 catcgcagaa gctgccttta ataaaggcga aacagcgatg accatcaacg gcccgtgggc
     2221 atggtccaac atcgacacca gcaaagtgaa ttatggtgta acggtactgc cgaccttcaa
     2281 gggtcaacca tccaaaccgt tcgttggcgt gctgagcgca ggtattaacg ccgccagtcc
     2341 gaacaaagag ctggcaaaag agttcctcga aaactatctg ctgactgatg aaggtctgga
     2401 agcggttaat aaagacaaac cgctgggtgc cgtagcgctg aagtcttacg aggaagagtt
     2461 ggcgaaagat ccacgtattg ccgccaccat ggaaaacgcc cagaaaggtg aaatcatgcc
     2521 gaacatcccg cagatgtccg ctttctggta tgccgtgcgt actgcggtga tcaacgccgc
     2581 cagcggtcgt cagactgtcg atgaagccct gaaagacgcg cagactaatt cgagctcgaa
     2641 caacaacaac aataacaata acaacaacct cgggatcgag ggaaggattt caactagtgg
     2701 atctggtggt ggtggcggtt ccatgagcga gaatctttat tttcagggat ccatgaatat
     2761 ccaagagcag ggtttcccct tggacctcgg agcaagtttc accgaagatg ctccccgacc
     2821 cccagtgcct ggtgaggagg gagaactggt gtccacagac ccgaggcccg ccagctacag
     2881 tttctgctcc gggaaaggtg ttggcattaa aggtgagact tcgacggcca ctccgaggcg
     2941 ctcggatctg gacctggggt atgagcctga gggcagtgcc tcccccaccc caccatactt
     3001 gaagtgggct gagtcactgc attccctgct ggatgaccaa gatgggataa gcctgttcag
     3061 gactttcctg aagcaggagg gctgtgccga cttgctggac ttctggtttg cctgcactgg
     3121 cttcaggaag ctggagccct gtgactcgaa cgaggagaag aggctgaagc tggcgagagc
     3181 catctaccga aagtacattc ttgataacaa tggcatcgtg tcccggcaga ccaagccagc
     3241 caccaagagc ttcataaagg gctgcatcat gaagcagctg atcgatcctg ccatgtttga
     3301 ccaggcccag accgaaatcc aggccactat ggaggaaaac acctatccct ccttccttaa
     3361 gtctgatatt tatttggaat atacgaggac aggctcggag agccccaaag tctgtagtga
     3421 ccagagctct gggtcaggga cagggaaggg catatctgga tacctgccga ccttaaatga
     3481 agatgaggaa tggaagtgtg accaggacat ggatgaggac gatggcagag acgctgctcc
     3541 ccccggaaga ctccctcaga agctgctcct ggagacagct gccccgaggg tctcctccag
     3601 tagacggtac agcgaaggca gagagttcag gtatggatcc tggcgggagc cagtcaaccc
     3661 ctattatgtc aatgccggct atgccctggc cccagccacc agtgccaacg acagcgagca
     3721 gcagagcctg tccagcgatg cagacaccct gtccctcacg gacagcagcg tggatgggat
     3781 ccccccatac aggatccgta agcagcaccg cagggagatg caggagagcg tgcaggtcaa
     3841 tgggcgggtg cccctacctc acattccccg cacgtaccgg gtgccgaagg aggtccgcgt
     3901 ggagcctcag aagttcgcgg aggagctcat ccaccgcctg gaggctgtgc agcgcacgcg
     3961 ggaggccgag gagaagctgg aggagcggct gaagcgcgtg cgcatggagg aggaaggtga
     4021 ggacggcgat ccatcgtcag ggcccccagg gccgtgtcac aagctgcctc ccgcccccgc
     4081 ttggcaccac ttcccgcccc gctgtgtgga catgggctgt gccgggctcc gggatgcaca
     4141 cgagaagtca ggggcgaagc tggacgcggc cggcctgcac caccaccgac acgtccacca
     4201 ccacgtccac cacagcacag cccggcccaa ggagcaggtg gaggccgagg ccacccgcag
     4261 ggcccagagc agcttcgcct ggggcctgga accacacagc catggggcaa ggtcccgagg
     4321 ctactcagag agtgttggcg ctgcccccaa cgccagtgat ggcctcgccc acagtgggaa
     4381 ggtgggcgtg gcgtgcaaaa gaaatgccaa gaaggctgag tcggggaaga gcgccagcac
     4441 cgaggtgcca ggtgcctcgg aggatgcgga gaagaaccag aaaatcatgc agtggatcat
     4501 tgagggggaa aaggagatca gcaggcaccg caggaccggc cacgggtctt cggggacgag
     4561 gaagccacag ccccatgaga actccagacc cttgtccctt gagcacccct gggccggccc
     4621 tcagctccgg acctccgtgc agccctccca cctcttcatc caagacccca ccatgccacc
     4681 ccacccagct cccaaccccc taacccagct ggaggaggcg cgccgacgtc tggaggagga
     4741 agaaaagaga gccagccgag caccctccaa gcagaggaca agatcgcaga ggaaggtggg
     4801 cggcgggagt gcccagccgt gtgacagcat cgttgtggcg tactacttct gcggggaacc
     4861 catcccctac cgcaccctgg tgaggggccg cgctgtcacc ctgggccagt tcaaggagct
     4921 gctgaccaaa aagggcagct acagatacta cttcaagaaa gtgagcgacg agtttgactg
     4981 tggggtggtg tttgaggagg ttcgagagga cgaggccgtc ctgcccgtct ttgaggagaa
     5041 gatcatcggc aaagtggaga aggtggacag cggccgccac catcaccacc accactaggg
     5101 gccgcactcg agaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     5161 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     5221 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggca
     5281 gcttggctgt tttggcggat gagataagat tttcagcctg atacagatta aatcagaacg
     5341 cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga
     5401 ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca
     5461 tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     5521 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg
     5581 gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat
     5641 aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc
     5701 tacaaactct tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     5761 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     5821 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     5881 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     5941 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg tttcccaatg
     6001 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     6061 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     6121 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     6181 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     6241 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     6301 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     6361 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     6421 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     6481 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     6541 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     6601 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     6661 taactgtcag accaagttta ctcatatata ctttagattg atttaccccg gttgataatc
     6721 agaaaagccc caaaaacagg aagattgtat aagcaaatat ttaaattgta aacgttaata
     6781 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     6841 aaatcggcaa aatcccttat aaatcaaaag aatagcccga gatagggttg agtgttgttc
     6901 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     6961 ccgtctatca gggcgatggc ccactacgtg aaccatcacc caaatcaagt tttttggggt
     7021 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     7081 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     7141 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     7201 cgccgctaca gggcgcgtaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     7261 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     7321 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     7381 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     7441 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     7501 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     7561 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     7621 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     7681 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     7741 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7801 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7861 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7921 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     7981 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     8041 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     8101 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     8161 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
     8221 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     8281 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     8341 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat
     8401 cagcgtggtc gtgcagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga
     8461 gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt
     8521 tttcctgttt ggtcactgat gcctccgtgt aatggggatt tctgttcatg ggggtaatga
     8581 taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt
     8641 tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa
     8701 tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc
     8761 agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt
     8821 ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg
     8881 ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag
     8941 taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc
     9001 gtggccagga cccaacgctg cccgaaattc c
//
