LOCUS       sequence-308815-        8689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CD4 CRISPRa control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8689)
  AUTHORS   Orchard RC, Sullender ME, Dunlap BF, Balce DR, Doench JG, Virgin HW
  TITLE     Identification of Antinorovirus Genes in Human Cells Using 
            Genome-Wide CRISPR Activation Screening.
  JOURNAL   J Virol. 2018 Dec 10;93(1). pii: JVI.01324-18. doi: 
            10.1128/JVI.01324-18. Print 2019 Jan 1.
  PUBMED    30305350
REFERENCE   2  (bases 1 to 8689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2018-12-10"; volume: "93(1). pii: JVI.01324-18. doi"; pages: 
            " 10.1128/JVI.01324-18. Print 2019 Jan 1"
COMMENT     Sequence Label: pXPR_502_sgCD4
FEATURES             Location/Qualifiers
     source          1..8689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    88..209
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(125..144)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     179..198
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      249..384
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(404..423)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(405..423)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(433..450)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(433..449)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(442..464)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      591..1046
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(678..697)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     888..909
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1072..1176
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1177..1245,1246..2037)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1395..1414)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2208..2796
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2697..2716
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2950..2967
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3084..3105
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3120..3125,3126..3143,3144..3150)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3158..3174
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3163..3185
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3182..3198
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3182..3198
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3217..3237
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3219..3237
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3265..3491
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3492..3672
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3719..3844
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4337..4570
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4755..4799
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4977..5225
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4977..4997
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5148..5167
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    5494..5611
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5660..6170
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6089..6107
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    complement(6635..6659)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6709..6729
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6745..7287
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             7312..7683
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             7699..7752
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7780..8376
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7780..7799)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8276..8296
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             join(8466..8689,1..10)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca
       61 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
      121 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
      181 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
      241 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
      301 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
      361 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
      421 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
      481 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
      541 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
      601 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
      661 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
      721 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
      781 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
      841 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
      901 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
      961 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     1021 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     1081 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1141 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     1201 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     1261 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     1321 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     1381 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     1441 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     1501 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     1561 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     1621 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     1681 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     1741 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     1801 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     1861 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     1921 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     1981 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     2041 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     2101 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     2161 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2221 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2281 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2341 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2401 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2461 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2521 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2581 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2641 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2701 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     2761 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     2821 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     2881 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     2941 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     3001 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     3061 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     3121 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     3181 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     3241 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
     3301 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
     3361 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
     3421 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc
     3481 gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     3541 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     3601 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     3661 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     3721 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     3781 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     3841 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     3901 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     3961 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     4021 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     4081 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     4141 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
     4201 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     4261 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     4321 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     4381 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     4441 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     4501 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     4561 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     4621 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     4681 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     4741 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     4801 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     4861 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     4921 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccagagagg
     4981 gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt agagagataa
     5041 ttagaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
     5101 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
     5161 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
     5221 acaccgatgt tccctgagag cctggggttt aagagctatg ctgcgaatac gagggagcag
     5281 acgatatggc gtcgctcctc tccacgagag catatgggct ccgtggtctc gtacaccatc
     5341 agggtacgta tcagacacca tcagggtctg ctcgtattcg cagcatagca agtttaaata
     5401 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttgaattcta
     5461 gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     5521 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     5581 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     5641 actttggcgc cggctcgagg gggcccgggt tgcgcctttt ccaaggcagc cctgggtttg
     5701 cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt
     5761 ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg
     5821 gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg
     5881 cgtgccggac gtgacaaacg gaagccgcac gtcttactag taccctcgca gacggacagc
     5941 gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag
     6001 cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg
     6061 tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca
     6121 cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggatccaa
     6181 cttaagcttg ccaccatgtc gcggaggcgg cacagctacg agaacgatgg tggacaacct
     6241 cacaaaagga ggaagacgtc tatgctagcc tccaaaacca tcgttctttc ggtcggcgag
     6301 gctactcgca ctctgactga gatccagtcc accgcagacc gtcagatctt cgaagagaag
     6361 gtcgggcctc tggtgggtcg gctgcgcctc acggcttcgc tccgtcaaaa cggagccaag
     6421 accgcgtatc gagtcaacct aaaactggat caggcggacg tcgttgattc cggacttccg
     6481 aaagtgcgct acactcaggt atggtcgcac gacgtgacaa tcgttgcgaa tagcaccgag
     6541 gcctcgcgca aatcgttgta cgatttgacc aagtccctcg tcgcgacctc gcaggtcgaa
     6601 gatcttgtcg tcaaccttgt gccgctgggc cgttcttcaa ctttcttgta caaagtgggc
     6661 ggaggaggtg gaagcggagg aggaggaagc ggaggaggag gtagcggacc taagaaaaag
     6721 aggaaggtgg cggccgctgg taccccttca gggcagatca gcaaccaggc cctggctctg
     6781 gcccctagct ccgctccagt gctggcccag actatggtgc cctctagtgc tatggtgcct
     6841 ctggcccagc cacctgctcc agcccctgtg ctgaccccag gaccacccca gtcactgagc
     6901 gctccagtgc ccaagtctac acaggccggc gaggggactc tgagtgaagc tctgctgcac
     6961 ctgcagttcg acgctgatga ggacctggga gctctgctgg ggaacagcac cgatcccgga
     7021 gtgttcacag atctggcctc cgtggacaac tctgagtttc agcagctgct gaatcagggc
     7081 gtgtccatgt ctcatagtac agccgaacca atgctgatgg agtaccccga agccattacc
     7141 cggctggtga ccggcagcca gcggcccccc gaccccgctc caactcccct gggaaccagc
     7201 ggcctgccta atgggctgtc cggagatgaa gacttctcaa gcatcgctga tatggacttt
     7261 agtgccctgc tgtcacagat ttcctctagt gggcagggag gaggtggaag cggcttcagc
     7321 gtggacacca gtgccctgct ggacctgttc agcccctcgg tgaccgtgcc cgacatgagc
     7381 ctgcctgacc ttgacagcag cctggccagt atccaagagc tcctgtctcc ccaggagccc
     7441 cccaggcctc ccgaggcaga gaacagcagc ccggattcag ggaagcagct ggtgcactac
     7501 acagcgcagc cgctgttcct gctggacccc ggctccgtgg acaccgggag caacgacctg
     7561 ccggtgctgt ttgagctggg agagggctcc tacttctccg aaggggacgg cttcgccgag
     7621 gaccccacca tctccctgct gacaggctcg gagcctccca aagccaagga ccccactgtc
     7681 tccgatatcg gcagtggaga gggcagagga agtctgctaa catgcggtga cgtcgaggag
     7741 aatcctggcc caggtaccac agatccaccg gagcttacca tgaccgagta caagcccacg
     7801 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     7861 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     7921 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     7981 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     8041 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     8101 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     8161 gtcggcgttt cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     8221 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     8281 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     8341 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccgcta gctgaacgcg ttaagtcgac
     8401 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     8461 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     8521 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8581 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8641 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaa
//
