LOCUS       sequence-308816-        9859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   Hara T, Takamura A, Kishi C, Iemura S, Natsume T, Guan JL, Mizushima
            N
  TITLE     FIP200, a ULK-interacting protein, is required for autophagosome 
            formation in mammalian cells.
  JOURNAL   J Cell Biol. 2008 May 5. 181(3):497-510.
  PUBMED    18443221
REFERENCE   2  (bases 1 to 9859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2008 May 5. 181(3):497-510."
COMMENT     Sequence Label: pMXs-IP-EGFP-ULK1
FEATURES             Location/Qualifiers
     source          1..9859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      1980..1989
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1986..1988,1989..1991,1992..2702)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1986..1988,1989..1991,1992..2702)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2031..2052)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2292..2311)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2639..2660
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(6008..6582,6583..6585,6586..6594)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6175..6192)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6402..6421
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     7094..7114
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7425..7894
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(7963..7980)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8134..8722)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8214..8233)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8886..9677,9678..9746))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9509..9528
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9747..9851)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 cccagtgtgg tggtacggga attcctgcag gcctcgaggg ccggcgcgcc gcggccgccg
     1981 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     2041 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     2101 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     2161 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     2221 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     2281 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     2341 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     2401 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     2461 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     2521 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     2581 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     2641 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     2701 agtccggact cagatctcga gctcaagctt cgaattcaat ggagccgggc cgcggcggcg
     2761 tcgagaccgt gggcaagttc gagttctctc gcaaggacct gattggacac ggcgccttcg
     2821 cggtggtctt caagggtcga caccgcgaga agcacgacct ggaggtggcc gtcaaatgca
     2881 ttaacaagaa gaaccttgcc aagtcccaaa cactgctggg aaaggaaatc aaaatcctga
     2941 aggaactaaa gcacgaaaac atcgtggcgc tgtatgactt ccaggaaatg gctaattctg
     3001 tctacctggt catggagtat tgtaatggtg gagacctggc tgactacctg cacactatgc
     3061 gcacactgag tgaagacact gtcaggcttt tcctacagca gatcgctggc gccatgcggc
     3121 tgctgcacag caagggcatc atccaccggg acctgaagcc ccagaacatc ctgctgtcca
     3181 accctggggg ccgccgggcc aaccccagca acatccgagt caagattgct gactttggat
     3241 tcgctcggta cctccagagc aacatgatgg cggccacact ctgtggttct cctatgtaca
     3301 tggctcctga ggtcattatg tcccagcact acgatggaaa ggctgacctg tggagcattg
     3361 gcaccattgt ctaccagtgt ctgacaggga aggccccttt tcaggccagc agccctcagg
     3421 atttgcgcct gttttatgag aagaacaaga cactagttcc tgccatcccc cgggagacat
     3481 cagctcccct gcggcagctg ctcctggctc tgttgcagcg gaaccacaag gaccgcatgg
     3541 actttgatga atttttccac caccctttct tggatgccag cacccccatc aagaaatccc
     3601 cacctgtgcc tgtgccctca tatccaagct cagggtctgg cagcagctcc agcagcagct
     3661 ctgcctccca cctggcctct ccaccgtccc tgggggagat gccacagcta cagaagaccc
     3721 ttacctcccc agccgatgct gctggctttc ttcagggctc ccgggactct ggtggcagca
     3781 gcaaagactc ctgtgacaca gatgactttg tcatggtccc agcccagttt ccaggtgatc
     3841 tagttgctga ggcagccagt gccaagcccc cacctgatag cctgctgtgt agtgggagct
     3901 cattggtggc ctctgctggc ctagagagcc acggccgtac cccctctccc tctccgacct
     3961 gcagcagctc tcccagcccc tctggccggc ctggcccctt ctccagcaac aggtacggtg
     4021 cctcggtccc cattcctgtc cccactcagg tgcacaatta ccagcgcatc gagcaaaacc
     4081 tgcaatcgcc cactcaacag cagacagccc ggtcctctgc catccgaagg tcagggagca
     4141 ccagccccct gggctttggc cgggccagcc catcaccccc ctcccacacc gatggggcca
     4201 tgctggccag gaagctgtca cttggaggtg gccgtcccta cacaccttct ccccaagtgg
     4261 gaaccatccc agagcgaccc agctggagca gagtgccctc cccacaagga gctgatgtgc
     4321 gggttggcag gtcaccacga cccggttcct ctgtgcctga gcactctcca agaaccactg
     4381 ggctgggctg ccgcctgcac agtgccccta acctgtccga cttccatgtt gtgcgtccca
     4441 agctgcctaa gcccccaaca gacccactgg gagccacctt tagcccaccc cagaccagcg
     4501 caccccagcc atgcccaggg ctacagtctt gccggccact gcgtggctca cctaagctgc
     4561 ctgacttcct acagcggagt cccctacccc ccatcctagg ctctcctacc aaggccgggc
     4621 cctcctttga cttccccaaa acccccagct ctcagaattt gctgaccctg ttggctaggc
     4681 agggggtagt aatgacacca cctcggaacc gtacactgcc tgacctctcc gaggccagtc
     4741 ctttccatgg ccagcagctg ggctctggcc ttcggcccgc tgaagacacc cggggtccct
     4801 ttggacggtc cttcagcacc agccgcatta cggacctgct gcttaaggct gcatttggga
     4861 ctcaggcctc tgactcaggc agcacagaca gcctacagga gaaacctatg gagattgctc
     4921 cctctgctgg ctttggaggg actctgcatc caggagctcg tggtggaggg gccagcagcc
     4981 cagcacctgt ggtatttact gtaggctccc cacccagtgg tgccacccca ccccagagta
     5041 cccgtaccag aatgttctca gtgggctctt ccagctccct gggctctact ggctcctcct
     5101 ctgcccgcca cttagtgcct ggggcctgtg gagaggcccc ggagctttct gccccaggcc
     5161 actgctgtag ccttgctgac ccccttgctg ccaacttgga gggggctgtg accttcgagg
     5221 ctcctgacct cccagaggag accctcatgg agcaagagca cacggaaact ctacacagtc
     5281 tgcgcttcac actagcgttt gcacagcaag ttctggagat tgcagccctg aagggaagtg
     5341 ccagtgaggc cgccggtggc cctgagtacc agctccagga aagtgtggtg gctgaccaga
     5401 tcagtcagtt gagccgagag tggggctttg cagagcaact ggttctgtac ttgaaggtgg
     5461 ctgagctgct gtcctcaggc ctacagactg ccattgacca gattcgagct ggcaaactct
     5521 gcctttcatc tactgtgaag caggtggtac gcagactaaa tgagctgtac aaggccagcg
     5581 tggtatcctg ccagggcctc agcttgcgac ttcagcgctt ctttctggac aaacaacggc
     5641 tgctggacgg gatccatggt gtcactgcag agcggctcat cctcagccat gctgtgcaaa
     5701 tggtacaatc agctgccctt gatgagatgt tccagcaccg agagggctgt gtaccgagat
     5761 atcacaaagc cctgctattg ctggaggggt tgcagcacac tctcacggac caggcagaca
     5821 ttgagaacat tgccaaatgc aagctgtgca ttgagaggag actctcggcc ctgctgagtg
     5881 gtgtctatgc ctgatgaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc
     5941 tagataactg atcataatca gccataccac atttgtaggc ggccgctacg taaattccgc
     6001 cccccccccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     6061 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     6121 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     6181 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     6241 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     6301 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     6361 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     6421 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     6481 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     6541 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatggtt
     6601 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     6661 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     6721 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     6781 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     6841 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     6901 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     6961 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     7021 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     7081 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     7141 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaccgtcac cgccgacgtc
     7201 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgagtcgac
     7261 gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg
     7321 taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact
     7381 gagaatagag aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa
     7441 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc
     7501 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
     7561 aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt
     7621 tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt
     7681 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc
     7741 cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat
     7801 aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct
     7861 gagtgattga ctacccgtca gcgggggtct ttcacagttg cattaatgaa tcggccaacg
     7921 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     7981 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     8041 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     8101 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     8161 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     8221 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     8281 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     8341 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8401 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8461 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8521 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     8581 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8641 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     8701 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     8761 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     8821 ctagatcctt ttgcggccgg ccgcaaatca atctaaagta tatatgagta aacttggtct
     8881 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8941 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9001 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9061 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9121 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9181 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9241 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9301 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9361 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9421 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9481 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9541 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9601 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9661 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9721 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9781 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9841 ggggttccgc gcacatttc
//
