LOCUS       sequence-308819-        8689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CD45 CRISPRa control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8689)
  AUTHORS   Orchard RC, Sullender ME, Dunlap BF, Balce DR, Doench JG, Virgin HW
  TITLE     Identification of Antinorovirus Genes in Human Cells Using 
            Genome-Wide CRISPR Activation Screening.
  JOURNAL   J Virol. 2018 Dec 10;93(1). pii: JVI.01324-18. doi: 
            10.1128/JVI.01324-18. Print 2019 Jan 1.
  PUBMED    30305350
REFERENCE   2  (bases 1 to 8689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2018-12-10"; volume: "93(1). pii: JVI.01324-18. doi"; pages: 
            " 10.1128/JVI.01324-18. Print 2019 Jan 1"
COMMENT     Sequence Label: pXPR_502_sgCD45
FEATURES             Location/Qualifiers
     source          1..8689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     108..125
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    242..263
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(278..283,284..301,302..308)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    316..332
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     321..343
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     340..356
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     340..356
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     375..395
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        377..395
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        423..649
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             650..830
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    877..1002
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1495..1728
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1913..1957
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2135..2383
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2135..2155
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2306..2325
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    2652..2769
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2818..3328
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3247..3265
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    complement(3793..3817)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3867..3887
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3903..4445
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             4470..4841
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             4857..4910
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4938..5534
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4938..4957)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5434..5454
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5624..5857
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5935..6056
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5972..5991)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6026..6045
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6096..6231
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6251..6270)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6252..6270)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6280..6297)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6280..6296)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6289..6311)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6438..6893
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6525..6544)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6735..6756
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6919..7023
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7024..7092,7093..7884)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7242..7261)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8055..8643
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8544..8563
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
       61 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
      121 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
      181 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
      241 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
      301 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
      361 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
      421 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
      481 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      541 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      601 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      661 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      721 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      781 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      841 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      901 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      961 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1021 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1081 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1141 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1201 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1261 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1321 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     1381 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1441 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1501 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1561 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1621 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1681 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1741 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1801 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1861 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1921 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1981 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2041 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2101 tcagacccac ctcccaaccc cgaggggacc cagagagggc ctatttccca tgattccttc
     2161 atatttgcat atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa
     2221 cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc
     2281 agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt
     2341 cgatttcttg gctttatata tcttgtggaa aggacgaaac accggttgtt ctaagtcagt
     2401 agaagtttaa gagctatgct gcgaatacga gggagcagac gatatggcgt cgctcctctc
     2461 cacgagagca tatgggctcc gtggtctcgt acaccatcag ggtacgtatc agacaccatc
     2521 agggtctgct cgtattcgca gcatagcaag tttaaataag gctagtccgt tatcaacttg
     2581 aaaaagtggc accgagtcgg tgcttttttt gaattctaga tcttgagaca aatggcagta
     2641 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     2701 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     2761 caaaattttc gggtttatta cagggacagc agagatccac tttggcgccg gctcgagggg
     2821 gcccgggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg
     2881 gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt
     2941 tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc
     3001 tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga
     3061 agccgcacgt cttactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg
     3121 ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg
     3181 ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc
     3241 cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt
     3301 tgaccgaatc accgacctct ctccccaggg ggatccaact taagcttgcc accatgtcgc
     3361 ggaggcggca cagctacgag aacgatggtg gacaacctca caaaaggagg aagacgtcta
     3421 tgctagcctc caaaaccatc gttctttcgg tcggcgaggc tactcgcact ctgactgaga
     3481 tccagtccac cgcagaccgt cagatcttcg aagagaaggt cgggcctctg gtgggtcggc
     3541 tgcgcctcac ggcttcgctc cgtcaaaacg gagccaagac cgcgtatcga gtcaacctaa
     3601 aactggatca ggcggacgtc gttgattccg gacttccgaa agtgcgctac actcaggtat
     3661 ggtcgcacga cgtgacaatc gttgcgaata gcaccgaggc ctcgcgcaaa tcgttgtacg
     3721 atttgaccaa gtccctcgtc gcgacctcgc aggtcgaaga tcttgtcgtc aaccttgtgc
     3781 cgctgggccg ttcttcaact ttcttgtaca aagtgggcgg aggaggtgga agcggaggag
     3841 gaggaagcgg aggaggaggt agcggaccta agaaaaagag gaaggtggcg gccgctggta
     3901 ccccttcagg gcagatcagc aaccaggccc tggctctggc ccctagctcc gctccagtgc
     3961 tggcccagac tatggtgccc tctagtgcta tggtgcctct ggcccagcca cctgctccag
     4021 cccctgtgct gaccccagga ccaccccagt cactgagcgc tccagtgccc aagtctacac
     4081 aggccggcga ggggactctg agtgaagctc tgctgcacct gcagttcgac gctgatgagg
     4141 acctgggagc tctgctgggg aacagcaccg atcccggagt gttcacagat ctggcctccg
     4201 tggacaactc tgagtttcag cagctgctga atcagggcgt gtccatgtct catagtacag
     4261 ccgaaccaat gctgatggag taccccgaag ccattacccg gctggtgacc ggcagccagc
     4321 ggccccccga ccccgctcca actcccctgg gaaccagcgg cctgcctaat gggctgtccg
     4381 gagatgaaga cttctcaagc atcgctgata tggactttag tgccctgctg tcacagattt
     4441 cctctagtgg gcagggagga ggtggaagcg gcttcagcgt ggacaccagt gccctgctgg
     4501 acctgttcag cccctcggtg accgtgcccg acatgagcct gcctgacctt gacagcagcc
     4561 tggccagtat ccaagagctc ctgtctcccc aggagccccc caggcctccc gaggcagaga
     4621 acagcagccc ggattcaggg aagcagctgg tgcactacac agcgcagccg ctgttcctgc
     4681 tggaccccgg ctccgtggac accgggagca acgacctgcc ggtgctgttt gagctgggag
     4741 agggctccta cttctccgaa ggggacggct tcgccgagga ccccaccatc tccctgctga
     4801 caggctcgga gcctcccaaa gccaaggacc ccactgtctc cgatatcggc agtggagagg
     4861 gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa tcctggccca ggtaccacag
     4921 atccaccgga gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     4981 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     5041 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     5101 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gccgtggcgg
     5161 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     5221 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     5281 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtttcg cccgaccacc
     5341 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     5401 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     5461 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     5521 gcaagcccgg tgccgctagc tgaacgcgtt aagtcgactt taagaccaat gacttacaag
     5581 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     5641 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     5701 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     5761 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     5821 ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat
     5881 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     5941 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6001 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6061 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     6121 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6181 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     6241 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     6301 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     6361 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     6421 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     6481 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     6541 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     6601 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     6661 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     6721 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     6781 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     6841 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     6901 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     6961 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7021 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7081 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7141 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7201 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     7261 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     7321 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     7381 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7441 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     7501 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     7561 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     7621 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     7681 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     7741 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     7801 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     7861 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     7921 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     7981 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8041 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8101 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8161 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     8221 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     8281 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     8341 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     8401 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8461 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     8521 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     8581 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     8641 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggc
//
