LOCUS       sequence-308828-        6781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human synapsin-1 promoter; Flp-dependent rM3D(Gs) DREADD-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6781)
  TITLE     Unpublished
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-hSyn-fDIO-rM3D(Gs)-mCherry-WPREpA
FEATURES             Location/Qualifiers
     source          1..6781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        155..602
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    640..673
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(766..1476)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1044..1063)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1313..1331
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      1473..1482
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2746..2779)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    2888..3476
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2941..2961)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3359..3370)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3508..3984
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3707..3726)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4024..4164
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4024..4153
                     /label=AAV2 ITR
     rep_origin      4239..4694
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4326..4345)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4536..4557
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4711..4730)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4830..4852
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4890..4908)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4976..5080
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5081..5149,5150..5941)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5299..5318)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6112..6700
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6601..6620
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   join(6762..6781,1..110)
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt ggtcgcccgg cctcagtgag
       61 cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct gcggccgcac
      121 gcgtgtgtct agactgcaga gggccctgcg tatgagtgca agtgggtttt aggaccagga
      181 tgaggcgggg tgggggtgcc tacctgacga ccgaccccga cccactggac aagcacccaa
      241 cccccattcc ccaaattgcg catcccctat cagagagggg gaggggaaac aggatgcggc
      301 gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt gccttcgccc ccgcctggcg
      361 gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac gtcactcgcc ggtcccccgc
      421 aaactcccct tcccggccac cttggtcgcg tccgcgccgc cgccggccca gccggaccgc
      481 accacgcgag gcgcgagata ggggggcacg ggcgcgacca tctgcgctgc ggcgccggcg
      541 actcagcgct gcctcagtct gcggtgggca gcggaggagt cgtgtcgtgc ctgagagcgc
      601 agtcgagaag gtaccggatc ctctagagtc gactccggag aagttcctat tctctagaaa
      661 gtataggaac ttcgcagaat ggtagctgga ttgtagctgc tattagcaat atgaaacctc
      721 ttagaagttc ctattcttca aaaggtatag gaacttcggc gcgccttact tgtacagctc
      781 gtccatgccg ccggtggagt ggcggccctc ggcgcgttcg tactgttcca cgatggtgta
      841 gtcctcgttg tgggaggtga tgtccaactt gatgttgacg ttgtaggcgc cgggcagctg
      901 cacgggcttc ttggccttgt aggtggtctt gacctcagcg tcgtagtggc cgccgtcctt
      961 cagcttcagc ctctgcttga tctcgccctt cagggcgccg tcctcggggt acatccgctc
     1021 ggaggaggcc tcccagccca tggtcttctt ctgcattacg gggccgtcgg aggggaagtt
     1081 ggtgccgcgc agcttcacct tgtagatgaa ctcgccgtcc tgcagggagg agtcctgggt
     1141 cacggtcacc acgccgccgt cctcgaagtt catcacgcgc tcccacttga agccctcggg
     1201 gaaggacagc ttcaagtagt cggggatgtc ggcggggtgc ttcacgtagg ccttggagcc
     1261 gtacatgaac tgaggggaca ggatgtccca ggcgaagggc agggggccac ccttggtcac
     1321 cttcagcttg gcggtctggg tgccctcgta ggggcggccc tcgccctcgc cctcgatctc
     1381 gaactcgtgg ccgttcacgg agccctccat gtgcaccttg aagcgcatga actccttgat
     1441 gatggccatg ttatcctcct cgcccttgct caccatggtg gcgaccggtg gatccgccaa
     1501 ggcctgctcc ggcactcgct tgtgaaaaat gaccgactgt ctctgctggt actgctgttt
     1561 gcgcctcttc cttttgtcac actggcacaa gaggagcgtc ttgaaggtgg ttctgaatgt
     1621 tttgttgcac agggcatagc acacagggtt cacggtgctg ttgatatagc acagccagta
     1681 gcccagattc caataggttt tgggtatgca gctgtcacag aaggtgttca ccaggaccat
     1741 gatgttgtag ggggtccacg tgatgatgaa ggccagcaag atggcactga gcgtctgcag
     1801 agctttgtgt tccctcatgg ccatgacacg ggacgtcttc ctcttgctgg cacggccgtt
     1861 gccgaggatg ggctggtgtt gggggagcgg gggtggctgc ggctgctcct ggctgccata
     1921 gaaccggccc tcgcagcggt cgatcttcct gatctgctcc ttggcctccc ggtacacccg
     1981 ccagtataaa atagtcatga tggtgacagg catgtaaaag ccagcgatcg ccgtgccgaa
     2041 ggtgatggtg ggctcactca gaaactgaat gaaacattct cctgggggca cagttctctt
     2101 ccctacaaag tattgccaga acaagatggc aggagcccat aggacaaagg agatgaccca
     2161 agccagacca atcatcacac cagctcgagc cctggtcatc aggctctggt agcggaatgg
     2221 agaagtgatg gaaaagtacc tgtcaaagct gatgaccagc agattcatga cagaggcatt
     2281 gctggccaca cagtcaatgg agagccagag gtcgcaggct aagttcccca gtgcccaacg
     2341 gttcataatg atgtaggtag tgaacaggtt catggaaatg accccgatga tcaggtctgc
     2401 acaggccagg cttaagagga agtagttgtt gactgtcttc agctgtttgt tgaccttgaa
     2461 ggccacaatg acaaggatgt tgccaatgat ggtcaccaat gccaggaagc cagttaagaa
     2521 ggcaatgaag accacttgcc agatggtgtg acccccgaga gggtcgctgg aggtgtcgtt
     2581 tgaggagaaa ttcccagttt cttgtgaaat gttgtagctg cccaactgag tgactgtccc
     2641 caagggcagc cctgcctccg agggactgtg cacccaggaa gagctgatgt tgggaaacaa
     2701 aggcgaggtt gtactgttac tgtgcaaggt catggtggcg ctagcgaagt tcctatactt
     2761 tctagagaat aggaacttct tgccttaacc cagaaattat cactgttatt ctttagaatg
     2821 gtgcaaagag aagttcctat accttttgaa gaataggaac ttcgaattcg atatcaagct
     2881 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     2941 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     3001 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     3061 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     3121 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     3181 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     3241 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     3301 gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     3361 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     3421 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     3481 ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct
     3541 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     3601 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     3661 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     3721 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     3781 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     3841 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     3901 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     3961 accactgctc ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc
     4021 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     4081 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     4141 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     4201 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     4261 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc
     4321 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4381 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4441 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4501 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4561 aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt
     4621 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     4681 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     4741 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     4801 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     4861 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     4921 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     4981 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5041 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5101 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5161 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5221 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5281 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     5341 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5401 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5461 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     5521 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     5581 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     5641 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     5701 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     5761 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     5821 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     5881 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     5941 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6001 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6061 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6121 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6181 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6241 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6301 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6361 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6421 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6481 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6541 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6601 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6661 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6721 tttacggttc ctggcctttt gctggccttt tgctcacatg tcctgcaggc agctgcgcgc
     6781 t
//
