LOCUS       sequence-308830-       12106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 position 2 containing the Arabidopsis Rps5a promoter, Cas9 
            (Zcasi), and the nos terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12106)
  AUTHORS   Grutzner R, Martin P, Horn C, Mortensen S, Cram EJ, Lee-Parsons CWT,
            Stuttmann J, Marillonnet S
  TITLE     High-efficiency genome editing in plants mediated by a Cas9 gene 
            containing multiple introns.
  JOURNAL   Plant Commun. 2020 Nov 23;2(2):100135. doi: 
            10.1016/j.xplc.2020.100135. eCollection 2021 Mar 8.
  PUBMED    33898975
REFERENCE   2  (bases 1 to 12106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun."; date: "2020-11-23"; volume: "2(2):100135. doi"; pages: " 
            10.1016/j.xplc.2020.100135. eCollection 2021 Mar 8"
COMMENT     Sequence Label: pAGM51323
FEATURES             Location/Qualifiers
     source          1..12106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    46..70
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(226..1374)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(1710..2298)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1790..1809)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2469..3260,3261..3329))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3092..3111
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3330..3434)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3521..4152
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    4244..4268
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6052..6072
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11805..11825
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      11852..12104
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 cgctactatt gtcttctgca cgaagtggtt taaactatca gtgtttgaca ggatatattg
       61 gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg
      121 aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt ccccagatca
      181 ggcgctggct gctgaacccc cagccggaac tgaccccaca aggccctagc gtttgcaatg
      241 caccaggtca tcattgaccc aggcgtgttc caccaggccg ctgcctcgca actcttcgca
      301 ggcttcgccg acctgctcgc gccacttctt cacgcgggtg gaatccgatc cgcacatgag
      361 gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat
      421 ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat atgaatttcg tgtagtggtc
      481 gccagcaaac agcacgacga tttcctcgtc gatcaggacc tggcaacggg acgttttctt
      541 gccacggtcc aggacgcgga agcggtgcag cagcgacacc gattccaggt gcccaacgcg
      601 gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac aggcattcct cggccttcgt
      661 gtaataccgg ccattgatcg accagcccag gtcctggcaa agctcgtaga acgtgaaggt
      721 gatcggctcg ccgatagggg tgcgcttcgc gtactccaac acctgctgcc acaccagttc
      781 gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg
      841 gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag
      901 ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc
      961 gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg
     1021 cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt
     1081 catagttcct cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct cctgttcaag
     1141 acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg gcagggggag ccagttgcac
     1201 gctgtcgcgc tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg actggaaggt
     1261 ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa
     1321 ccccgcgtcg atcagttctt gcctgtatgc cttccggtca aacgtccgat tcattcaccc
     1381 tccttgcggg attgccccga ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc
     1441 gcctggtgcc ttggtgtcca gataatccac cttatcggca atgaagtcgg tcccgtagac
     1501 cgtctggccg tccttctcgt acttggtatt ccgaatcttg ccctgcacga ataccagcga
     1561 ccccttgccc aaatacttgc cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa
     1621 gaagtcggtg cgctcctgct tgtcgccggc atcgttgcgc cacatctagg atctgccagg
     1681 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1741 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1801 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1861 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1921 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1981 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2041 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2101 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     2161 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2221 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2281 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2341 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2401 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2461 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2521 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2581 tctggcccca gtgctgcaat gataccgcga gaaccacgct caccggctcc agatttatca
     2641 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     2701 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2761 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     2821 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2881 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2941 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3001 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3061 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3121 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3181 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3241 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3301 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3361 tatcaaggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3421 ataggggttc cgcgcacgaa ttggccagcg ctgccatttt tggggtgagg ccgttcgcgg
     3481 ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt agcgggccgg gagggttcga
     3541 gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta
     3601 aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa agacaggtta gcggtggccg
     3661 aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc ctgtggacag cccctcaaat
     3721 gtcaataggt gcgcccctca tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc
     3781 cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc gcagggcact tatccccagg
     3841 cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag
     3901 gctggccagc tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt caacgccgcg
     3961 ccgggtgagt cggcccctca agtgtcaacg tccgcccctc atctgtcagt gagggccaag
     4021 ttttccgcga ggtatccaca acgccggcgg ccgcggtgtc tcgcacacgg cttcgacggc
     4081 gtttctggcg cgtttgcagg gccatagacg gccgccagcc cagcggcgag ggcaaccagc
     4141 ccggtgagcg tcgcaaagga gatcctgatc tgactgatgg gctgcctgta tcgagtggtg
     4201 attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg
     4261 gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact
     4321 ggggtggatg cagtgggccc cactctgtga agacaagcaa gaattcaagc ttggagctca
     4381 acttttgatt cgctatttgc agtgcacctg tggcgttcat cacatctttt gtgacactgt
     4441 ttgcactggt cattgctatt acaaaggacc ttcctgatgt tgaaggagat cgaaagtaag
     4501 taactgcacg cataaccatt ttctttccgc tctttggctc aatccatttg acagtcaaag
     4561 acaatgttta accagctccg tttgatatat tgtctttatg tgtttgttca agcatgttta
     4621 gttaatcatg cctttgattg atcttgaata ggttccaaat atcaaccctg gcaacaaaac
     4681 ttggagtgag aaacattgca ttcctcggtt ctggacttct gctagtaaat tatgtttcag
     4741 ccatatcact agctttctac atgcctcagg tgaattcatc tatttccgtc ttaactattt
     4801 cggttaatta aagcacgaac accattactg catgtagaag cttgataaac tatcgccacc
     4861 aatttatttt tgttgcgata ttgttacttt cctcagtatg cagctttgaa aagaccaacc
     4921 ctcttatcct ttaacaatga acaggttttt agaggtagct tgatgattcc tgcacatgtg
     4981 atcttggctt caggcttaat tttccaggta aagcattatg agatactctt atatctctta
     5041 catacttttg agataatgca caagaacttc ataactatat gctttagttt ctgcatttga
     5101 cactgccaaa ttcattaatc tctaatatct ttgttgttga tctttggtag acatgggtac
     5161 tagaaaaagc aaactacacc aaggtaaaat acttttgtac aaacataaac tcgttatcac
     5221 ggaacatcaa tggagtgtat atctaacgga gtgtagaaac atttgattat tgcaggaagc
     5281 tatctcagga tattatcggt ttatatggaa tctcttctac gcagagtatc tgttattccc
     5341 cttcctctag ctttcaattt catggtgagg atatgcagtt ttctttgtat atcattcttc
     5401 ttcttctttg tagcttggag tcaaaatcgg ttccttcatg tacatacatc aaggatatgt
     5461 ccttctgaat ttttatatct tgcaataaaa atgcttgtac caattgaaac accagctttt
     5521 tgagttctat gatcactgac ttggttctaa ccaaaaaaaa aaaaatgttt aatttacata
     5581 tctaaaagta ggtttaggga aacctaaaca gtaaaatatt tgtatattat tcgaatttca
     5641 ctcatcataa aaacttaaat tgcaccataa aattttgttt tactattaat gatgtaattt
     5701 gtgtaactta agataaaaat aatattccgt aagttaaccg gctaaaacca cgtataaacc
     5761 agggaacctg ttaaaccggt tctttactgg ataaagaaat gaaagcccat gtagacagct
     5821 ccattagagc ccaaacccta aatttctcat ctatataaaa ggagtgacat tagggttttt
     5881 gttcgtcctc ttaaagcttc tcgttttctc tgccgtctct ctcattcgcg cgacgcaaac
     5941 gatcttcagg tgatcttctt tctccaaatc ctctctcata actctgattt cgtacttgtg
     6001 tatttgagct cacgctctgt ttctctcacc acagcaatgg cttctagccc accgaagaag
     6061 aagcggaagg tcagctggaa aatggacaag aagtacagca ttggacttga tattggtacg
     6121 aactcagttg ggtgggccgt tatcaccgat gaatacaagg taccttcgaa gaaatttaaa
     6181 gtgctgggca acacagatag gcacagcatt aagaagaact tgatcggagc tctgctcttt
     6241 gactctggag aaaccgcgga ggcgacaagg cttaaacgta ctgcgaggag aaggtacact
     6301 cgcaggaaga acagaatctg ttatctccaa gagatcttta gcaacgagat ggcgaaggta
     6361 aggattttta tgatatagta tgcttatgta ttttgtactg aaagcatatc ctgcttcatt
     6421 gggatattac tgaaagcatt taactacatg taaactcact tgatgatcaa taaacttgat
     6481 tttgcaggtt gacgactcgt tcttccatcg cctcgaggaa tccttcctgg tagaggaaga
     6541 taagaaacac gagcgtcacc ccatctttgg gaatattgtt gacgaagtag cctatcatga
     6601 aaagtatccg actatatacc accttcgcaa gaagctggtg gactcaaccg ataaggcaga
     6661 ccttcggctc atatacctgg ctctcgcgca catgataaag tttcgtggcc atttcttgat
     6721 cgaaggggac ctcaacccgg ataactccga tgtggataaa ctgttcattc agctcgtcca
     6781 aacctacaat cagctgttcg aggagaaccc catcaatgca tcaggtaaca ttccttagtt
     6841 acctttcttt tctttttcca tcataagttt atagattgta catgctttga gatttttctt
     6901 tgcaaacaat ctcaggtgtc gacgccaagg caatactgtc tgccagactt tcgaagtcca
     6961 gacggcttga gaatctgatc gctcaattgc caggcgagaa gaagaacggc ttgttcggga
     7021 atctgattgc actgtctctg ggcctcaccc ctaacttcaa aagcaacttt gacctcgccg
     7081 aggacgcgaa gctgcagctg tcaaaggata catacgatga tgatctggac aatctgctcg
     7141 cccaaatagg tgctcttgaa attggaactc ttcttttgtt gtctaaacct atcaatttct
     7201 ttgcggaaat ttatttgaag ctgtagagtt aaaattgagt cttttaaact tttgtaggtg
     7261 atcagtatgc cgacctgttc ttggctgcca agaatctgtc agacgctatc ttgctcagtg
     7321 acattctgcg ggtcaacacg gagataacca aagcgccact tagcgcctcc atgatcaaga
     7381 ggtacgacga gcatcaccag gatctgaccc ttctgaaggc tttggttcgc cagcaactcc
     7441 ccgagaagta caaggagatt ttctttgacc aatcgaagaa tggctacgca gggtacattg
     7501 atggaggtaa gttgttactt atgattgttt tcctctctgc tacatgtatt ttgttgttca
     7561 tttctgtaag atataagaat tgagttttcc tctgatgata ttattaggtg caagtcagga
     7621 ggaattctac aaattcatca agcctattct ggaaaagatg gacggtacag aggagctgct
     7681 cgttaaattg aaccgcgaag atttgcttcg gaagcagcgt accttcgaca atggcagcat
     7741 accgcaccag atccacctcg gtgagctgca tgctatcttg aggaggcaag aggacttcta
     7801 tccgttcctg aaagacaaca gagagaagat tgaaaagatc ctcacgttcc gcattcccta
     7861 ctatgtaggt tagtatcata tgaagaaata cctagtttca gttgatgaat gctattttct
     7921 gacctcagtt gttctctttt gagaattatt tcttttctaa tttgcctgat ttttctatta
     7981 attcattagg tccactcgca cgcgggaact cgcggtttgc gtggatgaca cgcaaatccg
     8041 aggagactat cacgccttgg aacttcgaag aggtcgtgga caagggtgcg agtgcacagt
     8101 ccttcatcga aaggatgacc aacttcgata agaatctccc aaatgagaaa gtcctgccca
     8161 agcatagtct cctgtacgaa tacttcacgg tctacaacga gctgacgaag gtgaaatatg
     8221 tgacggaggg gatgcgcaaa ccggccttcc tgtcaggtaa atcctggtcc acacttttac
     8281 gataaaaaca caagatttta aactatgaac tgatcaataa tcattcctaa aagaccacac
     8341 ttttgttttg tttctaaagt aatttttact gttataacag gtgagcagaa gaaggccatt
     8401 gtcgatctct tgttcaaaac caatcggaag gtcactgtga aacagcttaa agaggactac
     8461 tttaagaaga tcgaatgctt tgattctgtg gaaatcagcg gcgttgagga taggttcaat
     8521 gcctctcttg gcacatacca tgacctgttg aaaatcatca aggacaagga cttccttgac
     8581 aacgaggaga acgaggacat cctcgaggac atcgtgctga ctctcacgct gtttgaggac
     8641 agagaaatga tcgaggagcg ccttaagact tatgcgcatc tgttcgatga caaggtcatg
     8701 aagcagttga agaggaggag atatacaggt aagaggtcaa aaggtttccg caatgatccc
     8761 tctttttttg tttctctagt ttcaagaatt tgggtatatg actaacttct gagtgttcct
     8821 tgatgcatat ttgtgatgag acaaatgttt gttctatgtt ttaggttggg gaaggctctc
     8881 caggaagctc atcaacggca tccgcgacaa gcaatccggc aagactatac tggactttct
     8941 caaatccgac ggttttgcga atcggaactt catgcagctt attcacgatg actcactgac
     9001 cttcaaagaa gatatccaga aggcccaagt gtcaggtcag ggcgatagcc ttcacgaaca
     9061 catagccaac ctggctggat cgccagctat aaagaagggc atactgcaga cagtgaaggt
     9121 tgtggatgag ctggtgaagg taagttctgc atttggttat gctccttgca ttttaggtgt
     9181 tcgtcgcact tccatttcca tgaatagcta agattttttt tctctgcatt cattcttctt
     9241 gcctcagttc taactgtttg tggtattttt gttttaatta ttgctacagg tcatgggccg
     9301 ccataagccg gagaacatcg tcatcgagat ggcgagggaa aaccagacga ctcagaaagg
     9361 gcagaagaac tcacgggagc gcatgaagcg gatagaggaa ggcatcaagg agcttgggag
     9421 tcagattctg aaagagcacc cagtcgaaaa tactcaactc cagaacgaga agctgtacct
     9481 ctattacctc cagaatggga gagatatgta cgtcgaccaa gagctcgaca ttaacagact
     9541 ctccgactat gatgtggatc acattgtccc tcaatctttc ctgaaggacg atagtattga
     9601 caacaaggta aagcaactgt gttttaatca atttcttgtc aggatatatg gattataact
     9661 taatttttga gaaatctgta gtatttggcg tgaaatgagt ttgctttttg gtttctcccg
     9721 tgttataggt ccttacgcgc tcagacaaga accgcggaaa atccgacaat gtacccagcg
     9781 aggaggttgt gaagaagatg aagaactatt ggaggcagct tttgaatgct aagctcataa
     9841 cccaacggaa attcgacaat ctcacgaagg cagaaagggg cggactgtct gagctcgaca
     9901 aagccggctt catcaagcgc cagttggttg aaactcgtca gattacgaaa catgtggccc
     9961 agatactcga ttcgcgtatg aatacgaagt atgatgagaa tgacaaactt atcagggagg
    10021 taaaggtaaa gtttccaact ttcctttacc atatcaaact aaagttcgaa actttttatt
    10081 tgatcaactt caaggccacc cgatctttct attcctgatt aatttgtgat gaatccatat
    10141 tgacttttga tggttacgca ggtgatcacc ctcaagagca aactggttag tgacttccgg
    10201 aaggacttcc agttttacaa ggttcgcgag atcaacaact accatcatgc ccatgacgcc
    10261 tacctgaacg ccgttgttgg cactgctctc atcaagaagt atccgaaact ggagtctgag
    10321 tttgtgtacg gggattacaa ggtgtacgac gttaggaaga tgatcgcgaa gtcagaacaa
    10381 gagatcggca aggctaccgc gaaatacttc ttttactcga atatcatgaa cttcttcaag
    10441 acagagatca ctctggcgaa tggtgaaatc cggaagaggc ctctgatcga gacaaatggc
    10501 gaaacaggtc tgtctttcct atttcatatg tttaatccta ggaatttgat caattgattg
    10561 tatgtatgtc gatcccaaga ctttcttgtt cacttatatc ttaactctct ctttgctgtt
    10621 tcttgcaggt gagattgtct gggataaggg cagggatttt gcgactgtgc gtaaggttct
    10681 cagcatgccc caagtcaaca tagtcaagaa aacggaggtt caaaccggtg gtttctccaa
    10741 ggagtccatt ctccctaagc gcaactccga caaactgatt gcgaggaaga aggattggga
    10801 tccgaagaaa tacggaggct ttgatagccc taccgtggca tacagcgtac tggtagtggc
    10861 caaggtggag aagggcaaga gcaagaaact gaaaagcgtc aaggaactgc ttggaattac
    10921 cataatggaa aggtcctcgt tcgagaagaa tccgatcgac ttcctcgagg ctaaaggtaa
    10981 aatattggat gccagacgat attctttctt ttgatttgta actttttcct gtcaaggtcg
    11041 ataaatttta ttttttttgg taaaaggtcg ataatttttt tttggagcca ttatgtaatt
    11101 ttcctaatta actgaaccaa aattatacaa accaggttac aaagaggtga agaaagacct
    11161 cattatcaaa ctgcccaagt attcgctttt cgaattggaa aatggcagaa aacgcatgct
    11221 ggcatctgcc ggagaactgc agaagggcaa cgagctggca ttgcccagta agtacgtcaa
    11281 cttcctgtac ttggcctcac actatgagaa gctgaagggg tcaccagagg acaacgagca
    11341 gaagcagttg tttgtcgagc agcacaagca ctatcttgat gagatcatag agcagatcag
    11401 cgaattttcc aagcgggtca ttcttgcaga cgctaacctc gataaggtaa ggacttctca
    11461 tgaatattag tggcagatta gtgttgttaa agtctttggt tagataatcg atgcctccta
    11521 attgtccatg ttttactggt tttctacaat taaaggtgct ttccgcgtac aacaagcaca
    11581 gagataagcc gataagggaa caagcggaaa acatcatcca cctgttcaca ctgaccaatc
    11641 tgggagcccc agcagccttt aagtacttcg ataccactat cgacagaaag cgctacacat
    11701 caaccaagga agtgttggac gctaccctta ttcaccaatc tattacaggg ctctatgaga
    11761 caaggataga tctgtcgcag ttgggtggtg actctagggc tgacccaaag aagaagcgta
    11821 aagtctgagc ttgatcctct agagtcaagc agatcgttca aacatttggc aataaagttt
    11881 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
    11941 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
    12001 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
    12061 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcga
//
